Characteristic spectrum construction method and multi-index component content detection method of ligustri lucidi fructus medicinal material and processed products thereof

A characteristic map and construction method technology, applied in the field of quality detection of Chinese medicinal materials, can solve problems such as difficulty in adapting to the high efficiency of processing, low detection efficiency, and difficulty in reflecting the quality differences of wine products, so as to facilitate large-scale application and detection efficiency High, stable and specific effects

Pending Publication Date: 2021-12-10
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this traditional method can only identify raw products and commercially available wine products, and it is difficult to reflect the differences in the quality of wine products caused by different processing degrees. The detection time is as long as 1 hour, the detection efficiency is low, and it is difficult to adapt to processing. production efficiency

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  • Characteristic spectrum construction method and multi-index component content detection method of ligustri lucidi fructus medicinal material and processed products thereof
  • Characteristic spectrum construction method and multi-index component content detection method of ligustri lucidi fructus medicinal material and processed products thereof
  • Characteristic spectrum construction method and multi-index component content detection method of ligustri lucidi fructus medicinal material and processed products thereof

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preparation example Construction

[0089] Prepare reference substance solution and need testing solution, wherein, the preparation method of described reference substance solution comprises the steps of: taking 5-hydroxymethylfurfural reference substance, salidroside reference substance, echinacoside reference substance, special female The chastitylin reference substance and the privetin G13 reference substance are dissolved by adding the first solvent; the preparation method of the test solution comprises the following steps: taking the medicinal material of Ligustrum lucidum or its processed product, adding the second solvent to extract, and the extract Filter and get the continued filtrate;

[0090] The reference substance solution and the test solution were detected by ultra-high performance liquid chromatography, and the detection conditions of the ultra-high performance liquid chromatography included: the mobile phase A used was acetonitrile, and the mobile phase B was water; the elution method was gradien...

Embodiment 1

[0128] This embodiment is a method for establishing the UPLC characteristic spectrum of Ligustrum lucidum medicinal material and its processed decoction pieces.

[0129] Ligustrum lucidum medicinal materials: A total of 11 batches of Ligustrum lucidum medicinal materials that meet the requirements of the 2020 edition of the "Chinese Pharmacopoeia" were collected from Henan, Jiangsu, Shandong and other producing areas.

[0130] Processed Ligustrum lucidum wine: Take the medicinal materials of Ligustrum lucidum, remove impurities, and divide it into three parts, one part is crushed, and the other two parts are Ligustrum lucidum crushed decoction pieces; Put the decoction pieces in a container, dilute the rice wine with drinking water equivalent to 1 times the weight of rice wine, use 20kg of rice wine for every 100kg of decoction pieces of Ligustrum lucidum, and take it out after 2 to 4 hours of stuffing; one portion of Ligustrum lucidum crushed decoction pieces is steamed for 8....

Embodiment 2

[0217] This embodiment is a method for detecting the content of index components of the medicinal material of Ligustrum lucidum and its processed decoction pieces.

[0218] The triterpenoids, iridoid glycosides and phenylethanol glycosides contained in Ligustrum lucidum are its main medicinal components, and the main representative components are salidroside, privetin, privetin, privetin G13 and Oleonuezhenide et al. In the invention, salidroside, privetin and privetin G13 are used as content determination indexes to evaluate the privet fruit medicinal material and its processed decoction pieces.

[0219] 1. Chromatographic conditions and system suitability test

[0220] Octadecylsilane bonded silica gel was used as filler (ACQUITY UPLC BEH Shield RP18 Column, column length 100mm, inner diameter 2.1mm, particle size 1.7μm); acetonitrile was used as mobile phase A, and water was used as mobile phase B , carry out gradient elution according to the provisions in Table 1; the fl...

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Abstract

The invention relates to a characteristic spectrum construction method of a ligustri lucidi fructus medicinal material and processed products thereof. The characteristic spectrum construction method comprises the following steps that a reference substance solution and a test solution are prepared, wherein the reference substance solution is prepared by the steps of taking and dissolving a 5-hydroxymethylfurfural reference substance, a rhodioloside reference substance, an echinacoside reference substance, a specnuezhenide reference substance and a nuezhenide G13 reference substance with a first solvent, and the test solution is prepared by the steps of taking a ligustri lucidi fructus medicinal material or a processed product thereof, adding a second solvent for extraction, filtering an extracting solution, and taking a subsequent filtrate; and ultra-high performance liquid chromatography detection is carried out on the reference substance solution and the test solution, wherein the detection conditions of ultra-high performance liquid chromatography are as follows: the adopted mobile phase A is acetonitrile, the adopted mobile phase B is water, and the elution mode is gradient elution. The characteristic spectrum construction method can be used to efficiently identify the ligustri lucidi fructus medicinal material and the processed products thereof with different processing degrees.

Description

technical field [0001] The invention relates to the technical field of quality detection of Chinese medicinal materials, in particular to a method for constructing a characteristic map of medicinal materials of Ligustrum lucidum and its processed products, a method for detecting the content of multi-indicator components, and a characteristic map and multiple information of medicinal materials of Ligustrum lucidum and its processed products. Synchronous detection method of index component content. Background technique [0002] Ligustrum lucidum Ait. is the dry ripe fruit of Ligustrum lucidum Ait. Harvest when the fruit is ripe in winter, remove branches and leaves, steam or put in boiling water for a while, and then dry; or directly dry. Sweet in the mouth, bitter, cool in nature. Return liver, kidney channel. It has the effect of nourishing the liver and kidney, improving eyesight and black hair. It is used for liver and kidney yin deficiency, dizziness and tinnitus, sore...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/54G01N30/74
Inventor 邓李红姚晓璇林伟雄陈仕妍田清清钟文峰孙冬梅魏梅程学仁
Owner GUANGDONG YIFANG PHARMA
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