Breeding method for cotton virescent nuclear male sterile dual-purpose line Yt30

A technology of male sterile lines and male sterility, applied in botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of seed enterprises and farmers' losses, incomplete removal of cotton bolls, and inability to find plants, etc., and achieve reduction The effect of workload, ease of identification of hybrid purity, and simplification of breeding difficulty

Inactive Publication Date: 2017-08-08
TAICANG AGRI TECH EXTENSION CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem is that due to incomplete removal of cotton bolls on sterile plants or sterile plants before hybrid seed production, some male sterile plants are mixed in the F1 hybrids. tree", only flowering but not bolling, serious yield loss
If the rate of sterile plants is high (above 4%), farmers will claim compensation, the variety will be withdrawn from the market, and the losses of seed companies and farmers will be serious

Method used

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  • Breeding method for cotton virescent nuclear male sterile dual-purpose line Yt30
  • Breeding method for cotton virescent nuclear male sterile dual-purpose line Yt30
  • Breeding method for cotton virescent nuclear male sterile dual-purpose line Yt30

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The breeding method of cotton bud yellow nuclear male sterile dual-purpose line Yt30 comprises the following steps: in the first year, the male sterile line Ning A controlled by a recessive nuclear gene is used as the female parent, and the space cotton excellent line material Tai 5018 is used as the father F 1 generation; the second year, for F 1 Generation selfing. In the third year, in F 2 In the population, the sterile plants were selected to be paired and crossed with the Yahuang material (genetic male sterile line selected from the parents). In the fourth year, pair bud yellow hybrid F 1 Generation selfing. Fifth year, in F 2 The Yahuang sterile plants were found in the population, and they were crossed with the Yahuang fertile plants in the same row. In the sixth year, a row of yellow buds was planted, numbered Yt30. The yellow bud rate of the row was 100%, the proportion of sterile plants was 38.7%, and the plant traits in the row were basically the same. ...

Embodiment 2

[0055] The breeding method of cotton bud yellow nucleus male sterile dual-purpose line Yt30 comprises the following steps: In 2011, Ning A (sterile plant) was used as the female parent, and the space cotton excellent line material Tai 5018 was used as the male parent to prepare F 1 generation. In 2012, on F 1 Generation selfing. In 2013, in F 2 In the population, the sterile plants were selected to be paired and crossed with the Yahuang material. In 2014, to Yahuang hybrid F 1 Generation selfing. In 2015, in F 2 The Yahuang sterile plants were found in the population, and they were crossed with the Yahuang fertile plants in the same row. In 2016, a row of yellow buds was planted, numbered Yt30. The yellow bud rate of the row was 100%, and the proportion of sterile plants was 38.7%. The plant traits in the row were basically the same.

[0056] In this example, it is a dual-purpose line for recessive genital sterility. The plant is medium to high, slightly loose, and has...

Embodiment 3

[0068] Application of Embodiment 3Yt30

[0069] 1. Yt30 breeding method

[0070] Yt30 is planted in the field with a density of 4000 plants / mu, seedlings are raised and transplanted, and the cultivation management method is the same as that of ordinary cotton. When flowering, male sterile plants and fertile plants are identified according to whether there is pollen in the anthers, and they are listed for marking. On the day of flowering, pollen from fertile plants was taken to pollinate sterile plants. From the first day of flowering until the effective flower termination period. The seed cotton in the hybrid cotton bolls on the sterile plants is harvested, and after being dried in the sun, the seeds are removed, develored with sulfuric acid and dried in the sun for use.

[0071] 2. The method of using Yt30 for cotton hybrid seed production

[0072] Yt30 is planted in the field, seedlings are raised and transplanted, the density is 6000 plants / mu, and the cultivation and m...

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Abstract

The invention discloses a breeding method for the dual-purpose cotton male sterile line Yt30 with yellow nucleus. The upland cotton male sterile lines Ning A, Tai 5018, and bud yellow mutant line (yellow leaves) are used as parents, and the Ning A recessive nuclear sterile line is used as parents. The breeding line is crossed with Tai 5018 with high combining ability and excellent traits, and then the excellent single plant is selected for crossing with the bud yellow mutant line. The yellow and male sterile plants were crossed with the Yahuang and fertile plants in the same row to obtain a new upland cotton bud yellow nucleus male sterile dual-purpose line Yt30. In the present invention, one parent with high combining ability and one parent with recessive bud yellow are selected, and the sterile lines are respectively crossed with the parents, and excellent single plants are selected from the separated offspring according to the traits such as yield, quality, stamen fertility, and leaf color, and the excellent and consistent plants are selected. In the lines, the male sterile plants marked by Bud Yellow were selected to be crossed with fertile plants, and a high combining ability nuclear male sterile line with recessive Bud Yellow was obtained.

Description

technical field [0001] The invention belongs to the field of cotton germplasm resources, in particular, the invention relates to a breeding method of cotton bud yellow nucleus male sterile dual-purpose line Yt30. Background technique [0002] Cotton is one of the main economic crops in my country, and it is mainly used to obtain important textile raw materials, cotton fiber, vegetable oil and protein resources, cottonseed oil and cottonseed protein. [0003] Conventional cotton breeding methods include: systematic breeding, that is, selecting excellent mutant individuals (single plants) or single bolls in the existing population of popularized varieties to cultivate new varieties; cross-breeding, that is, selecting suitable Parents, through crossing, are the genes that control the good traits of both parents in F 1 The combination is heterozygous in the population, and in F 1 F formed by selfing 2 In the segregation population, select individuals that meet the breeding ob...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 邓惠清唐灿明苏锦其姜利农季浩
Owner TAICANG AGRI TECH EXTENSION CENT
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