Method for detection, identification and content determination of folia eriobotryae or drug containing folia eriobotryae raw material

A detection method, loquat leaf technology, applied in the field of drug quality detection, to achieve the effect of saving detection costs

Active Publication Date: 2018-10-30
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The HPLC fingerprints of loquat leaf medicinal materials reported so far can evaluate the quality of traditional Chinese medicinal materials, but t

Method used

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  • Method for detection, identification and content determination of folia eriobotryae or drug containing folia eriobotryae raw material
  • Method for detection, identification and content determination of folia eriobotryae or drug containing folia eriobotryae raw material
  • Method for detection, identification and content determination of folia eriobotryae or drug containing folia eriobotryae raw material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 HPLC characteristic collection of illustrative plates of the present invention

[0059] 【Characteristic spectrum】Determined according to high performance liquid chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0512).

[0060]Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler (column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); 0.4% phosphoric acid solution is used as mobile phase A, and acetonitrile is used as mobile phase B. Perform gradient elution as specified in the table below; the flow rate is 1.0 mL per minute; the column temperature is 35°C; the detection wavelength is 300 nm. The number of theoretical plates should not be less than 5000 based on the chlorogenic acid peak.

[0061]

[0062] Preparation of reference solution: Take appropriate amount of reference substances of chlorogenic acid, neochlorogenic acid, and cryptochlorogenic acid, add 50...

Embodiment 2

[0068] The conditional screening test of embodiment 2 characteristic graph of the present invention

[0069] 1. Experimental instruments and materials

[0070] High performance liquid chromatography: Agilent 1260 high performance liquid chromatography, Shimadzu LC-20AD high performance liquid chromatography, Waters 2695-2996 high performance liquid chromatography;

[0071] Electronic balance: ME204E / 02, MS205DU, XP26 (Mettler-Toledo Instrument Co., Ltd.);

[0072] Ultrapure water machine: cell type 1810A (Shanghai Mole Scientific Instrument Co., Ltd.);

[0073] Ultrasonic cleaner: KQ5200DB type (600W, 40KHz; Kunshan Ultrasonic Instrument Co., Ltd.);

[0074] Chromatographic column: Agilent5HC-C18 250×4.6mm 5μm, Diamonsil C18 250×4.6mm 5μm, KromasilC18 5um 4.6×250mm.

[0075] Acetonitrile, methanol, and phosphoric acid are chromatographically pure, water is ultrapure water, and other reagents are analytically pure.

[0076] Chlorogenic acid (National Institute for Food and ...

Embodiment 3

[0177] Embodiment 3 Assay method of the present invention

[0178] 1 Instruments and reagents

[0179] High performance liquid chromatography: Agilent 1260 high performance liquid chromatography, Shimadzu LC-20AD high performance liquid chromatography, Waters2695-2996 high performance liquid chromatography;

[0180] Electronic balance: ME204E / 02, MS205DU, XP26 (Mettler-Toledo Instrument Co., Ltd.);

[0181] Ultrapure water machine: cell type 1810A (Shanghai Mole Scientific Instrument Co., Ltd.);

[0182] Ultrasonic cleaner: KQ5200DB type (600W, 40KHz; Kunshan Ultrasonic Instrument Co., Ltd.);

[0183] Chromatographic column: Agilent 5TC-C18(2) 250×4.6mm, phenomenonexLuna 5u C18(2) 100A250×4.60mm 5micron, kromasilC18 5um4.6×250mm.

[0184]Chlorogenic acid (National Institute for Food and Drug Control, batch number: 110753-201415, with a content of 96.2%).

[0185] Acetonitrile and phosphoric acid are chromatographically pure, water is ultrapure water, and other reagents are...

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Abstract

The invention provides a method for the detection of folia eriobotryae or drugs containing folia eriobotryae raw material. The detection method adopting HPLC (high performance liquid chromatography) fingerprint for detection includes the steps of: (1) preparing a test solution; (2) preparing a reference solution by taking chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid; (3) conducting precise drawing of the reference solution and the test solution into a liquid chromatograph and then performing elution with 0.4% of phosphoric acid-acetonitrile as a mobile phase. The quality detection method of the folia eriobotryae or the drugs containing the folia eriobotryae raw material has the advantages of being capable of performing content determination and feature mapping and saving inspection costs, and being capable of distinguishing between folia eriobotryae (medicinal materials, decoction pieces or formula granules) and honeyed folia eriobotryae (medicine, decoction piecesor formula granules) and conducting quality control of the whole process of folia eriobotryae or honeyed folia eriobotryae production.

Description

technical field [0001] The invention relates to a quality detection method of loquat leaves or medicines containing loquat leaves raw materials. Background technique [0002] Loquat leaves are the dried leaves of Rosaceae loquat Eriobotrya japonica (Thunb.) Lindl. It has the effects of clearing the lung and relieving cough, lowering the adverse flow and relieving vomiting. It is used for cough due to lung heat, dyspnea due to adverse Qi, stomach heat and vomiting, dysphoria and thirst. At present, the quality control of loquat leaves (honey loquat leaves) is mainly based on ursolic acid and oleanolic acid. [0003] At present, there are relevant literature reports using the method of HPLC fingerprinting to detect loquat leaves, such as: Zhang Changhao, Research on HPLC fingerprinting of loquat leaves, Shizhen National Medicine, Volume 18, No. 2, 2007, the chromatographic column is KromasilC 18 (4.6×200mm, 5μm), the mobile phase was methanol-0.1% glacial acetic acid aqueous...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 周厚成胡昌江李文兵许玲张玉婷刘聪张彩虹
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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