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Detection method forqualified pinellia tubers

A detection method, the technology of pinellia, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of the safety and effectiveness of pinellia medicinal materials, and it is difficult to distinguish counterfeit products of pinellia, achieving good application prospects, The effect of high accuracy and simple detection method

Active Publication Date: 2014-08-06
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in recent years, the market of pinellia medicinal materials has been relatively good, and there have been many counterfeit and confused products in its circulation, which has brought a great impact on the quality, safety and effectiveness of the medicinal materials of Pinellia.
Through research on the market of Chinese herbal medicines, it is found that the counterfeit product of Pinellia is mainly Shui Pinellia, and the main confused products include Arachnium chinensis, Araceae, and Pinellia shanzhu. However, the current Chinese Pharmacopoeia method is difficult to distinguish pinellia and its counterfeit products.

Method used

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  • Detection method forqualified pinellia tubers
  • Detection method forqualified pinellia tubers
  • Detection method forqualified pinellia tubers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 Detection method parameters are preferred

[0067] 1. Screening of sample preparation methods

[0068] 1. Experimental method

[0069] Sample preparation method 1: Take 2g of Pinellia Medicinalis (passed through a 40-mesh sieve), add 20ml of petroleum ether, extract by ultrasonic for 30min, discard the petroleum ether, add 20ml of 95% ethanol, extract by ultrasonic three times, combine the extracts and evaporate to dryness. Make up to 2ml.

[0070] Sample preparation method 2: Take 2 g of Pinellia ternata (passed through a 40-mesh sieve), add 20 ml of water for ultrasonic extraction for 30 minutes, take the supernatant, evaporate to dryness and dilute to 2 ml with water.

[0071] Sample preparation method 3: Take 2 g of Pinellia ternata (passed through a 40-mesh sieve), add 20 ml of methanol for ultrasonic extraction for 30 minutes, take the supernatant, evaporate to dryness and dilute to 2 ml with methanol.

[0072] Sample preparation method 4: Take 2 g o...

Embodiment 2

[0107] Example 2 Methodological investigation of the method of the present invention

[0108] Adopt the preferred method of embodiment 2, carry out methodological investigation:

[0109] 1. Precision test

[0110] Take the same sample powder of Pinellia sinensis, pass the powder through a No. 4 sieve, prepare samples according to the above method, inject 5 times, 10 μl each time, measure according to the above gradient conditions, and investigate the consistency of the retention time of each chromatographic peak and the retention time of each main chromatographic peak. The RSD of the chromatographic fingerprint was less than 2%; the similarity of the five spectra was analyzed by the similarity evaluation software, and the similarity between the measured chromatographic fingerprint and the obtained common pattern map were: 0.978, 0.989, 0.983, 0.992, 0.985, all of which were If it is greater than 0.97, it indicates that the instrument is stable and the precision is good. See ...

Embodiment 3

[0115] Example 3 The method of the present invention detects genuine Pinellia sinensis

[0116] 1. Experimental method

[0117] Adopt the preferred method of embodiment 2 to prepare the test solution, accurately draw 10 μl, inject into the liquid chromatograph respectively, measure according to the chromatographic conditions after screening, record the HPLC chart of 45min, and adopt the “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation” of the State Pharmacopoeia Commission System (2004A)” for data analysis and processing, matching the spectra of Pinellia ternata, Pinellia shui, Bai Fuzi and Huzhang Nanxing samples respectively to generate a control spectrum, and then automatically match the chromatographic peaks measured by the samples with the reference spectrum to generate The fingerprints of Pinellia, Shui Pinellia, Bai Fuzi and Huzhang Nanxing share the same pattern.

[0118] The standard substances adenosine and guanosine were used for calibration. The...

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Abstract

The invention discloses a detection method for qualified pinellia tubers. According to the method, determination is performed through HPLC (high performance liquid chromatography) fingerprints and the method comprises the operation steps as follows: (1) taking a to-be-detected sample, crushing the sample, performing extraction with water or methanol serving as a solvent, and preparing a test solution; (2) injecting the test solution into a high performance liquid chromatograph to be determined. Chromatographic conditions are as follows: the detection wavelength is in a range of 254-265 nm; a chromatographic column takes octadecylsilane as filler; and a mobile phase is eluted by a methanol-water system. The detection method for the qualified pinellia tubers is high in precision, good and accurate in reproducibility, convenient to operate, low in cost and broad in market application prospect.

Description

technical field [0001] The invention relates to a method for detecting genuine Pinellia, belonging to the field of traditional Chinese medicine. Background technique [0002] Pinellia originates from the dry tubers of Pinellia ternata (Thunb.) breit. Pinellia ternata resources are widely distributed in my country, except for Jilin, Heilongjiang, Inner Mongolia, Xinjiang, Qinghai, and Tibet, all other provinces and regions are distributed. [0003] In recent years, the market demand of Pinellia has continued to increase, while the wild resources have been decreasing year by year, coupled with the slow development of artificial cultivation of Pinellia, resulting in a sharp decline in both the resource reserves and output of Pinellia. In addition, in recent years, the market of Pinellia medicinal materials has been relatively good, and many counterfeit products and confounded products have appeared in its circulation, which has brought a great impact on the quality of Pinellia...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 李敏卢道会杨小艳敬勇
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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