Method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products

A fingerprint and construction method technology, which is applied in the field of construction of chromatographic fingerprints for quality control of traditional Chinese medicine herbal tea and herbal beverage series products, can solve problems such as being unfavorable for maintaining the reputation of plant beverages, lacking quality control standards, adverse to consumer rights and interests, safety and health, etc. question

Inactive Publication Date: 2012-10-03
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the "Hygienic Requirements for Plant Beverages" only set requirements for raw and auxiliary materials, sensory indicators, hygienic physical and chemical indicators, microbial indicators, and food additives for plant beverages, and did not reflect the quality control standards for effective chemical compo...

Method used

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  • Method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products
  • Method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products
  • Method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products

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Effect test

Embodiment 1

[0041] This embodiment includes the following steps:

[0042] (1) Sample treatment by freeze-drying-extraction method: Measure 20 mL of three kinds of herbal tea products of traditional Chinese medicine with the same raw materials, freeze-dry them at -40°C until dry, then add 20 mL of methanol to the obtained solid residue, power 150W, frequency Ultrasonic extraction at 40KHz for 30 min, centrifugation at 10 000 rpm for 10 min, supernatant, N 2 Blow dry, add 2 mL of methanol to the obtained residue, and ultrasonically reconstitute under the conditions of power 150W and frequency 40KHz;

[0043] (2) HPLC fingerprint analysis: Pass the sample processed in step (1) through a 0.45 μm organic membrane for HPLC analysis. The high performance liquid chromatography conditions are as follows:

[0044] Instrument: HPLC-UV

[0045] Chromatographic column:

[0046] Detection wavelength: 205 nm

[0047] Mobile phase: A. 0.1% phosphoric acid water B. 0.1% phosphoric acid acetonitrile ...

Embodiment 2

[0055] This embodiment includes the following steps:

[0056](1) Sample treatment by heat evaporation drying-extraction method: Measure 20 mL of three kinds of traditional Chinese herbal herbal teas with the same raw materials, evaporate to dryness on a water bath at 100 ℃, add 20 mL of acetonitrile to the obtained solid residue, under the conditions of power 150W and frequency 40KHz Ultrasonic extraction for 30 min, centrifugation at 10 000 rpm for 10 min, supernatant, N 2 Blow dry, add 2 mL of methanol to the obtained residue, and ultrasonically reconstitute under the conditions of power 150W and frequency 40KHz;

[0057] (2) HPLC fingerprint analysis: Pass the sample processed in step (1) through a 0.45 μm organic membrane for HPLC analysis. The high performance liquid chromatography conditions are as follows:

[0058] Instrument: HPLC-UV

[0059] Chromatographic column:

[0060] Detection wavelength: 205 nm

[0061] Mobile phase: A. 0.1% phosphoric acid water B. 0.1%...

Embodiment 3

[0069] This embodiment includes the following steps:

[0070] (1) Sample treatment by solid-phase extraction: wash 6 mL of methanol and 6 mL of water sequentially into a 500 mg / 6 mL C18 solid-phase extraction column for activation and equilibrium, and take 10 mL of three kinds of herbal teas of the same raw materials respectively Pass through a C18 small column, pump dry, and elute with 10 mL of methanol, and the eluate obtained is washed with N 2 Blow dry, and redissolve the obtained residue with 1 mL methanol at 2 000 rpm;

[0071] (2) HPLC fingerprint analysis: Pass the sample processed in step (1) through a 0.45 μm organic membrane for HPLC analysis. The high performance liquid chromatography conditions are as follows:

[0072] Instrument: HPLC-UV

[0073] Chromatographic column:

[0074] Detection wavelength: 205 nm

[0075] Mobile phase: A. 0.1% phosphoric acid water B. 0.1% phosphoric acid acetonitrile

[0076] Flow rate: 1 mL / min

[0077] Injection volume: 20 μ...

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Abstract

The invention discloses a method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products. The method comprises the steps of: (1) preprocessing a sample and processing the sample by one method selected from following methods: (1) a freeing, drying-extracting method, (2) a thermal evaporating, drying-extracting method, and (3) a solid-phase extraction method; (2) conducting HPLC (high performance liquid chromatography) fingerprint map analysis. According to the method, the quality control chromatography fingerprint maps of the traditional Chinese medicine herbal tea and herbal medicine beverage products built by the method disclosed by the invention can be used for carrying out macroscopic and fine constituent quality control on the product, and the blank that the Chinese herbal medicine beverage such as the traditional Chinese medicine herbal tea and herbal medicine herbal tea only has requirements on raw material and auxiliary material, sensory indexes and health indexes in quality control, but has no macroscopic quality control indexes of related phytochemical constituents at present is filled.

Description

technical field [0001] The invention relates to a construction method of quality control chromatographic fingerprints, in particular to a construction method of quality control chromatographic fingerprints of traditional Chinese herbal tea and herbal beverage series products. Background technique [0002] my country's beverage industry is a new industry developed after the reform and opening up. It started relatively late, but developed very rapidly. The total output of beverages in the country maintained a sustained and steady growth. [0003] As people's awareness of health increases, people begin to choose healthy drinks, and the demand for Chinese herbal teas and herbal drinks is also increasing. However, the current quality control of Chinese herbal teas and herbal beverages is based on the content of a certain component as an indicator, or focuses on sensory indicators, some physical and chemical indicators, microbial indicators, etc., and there has been no standard t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08G01N30/14
Inventor 陈波李彬王华吴青李媛
Owner HUNAN NORMAL UNIVERSITY
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