Establishing method for cordyceps fungus fingerprints and standard fingerprints

A standard fingerprint and fingerprint technology, applied in the field of drug analysis, can solve the problems of resource scarcity and achieve good reproducibility, good stability, and benefits for popularization and application

Active Publication Date: 2014-01-01
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Cordyceps sinensis belongs to the genus Cordyceps, and its anamorph is Hirsutella sinensis. It is the most medicinal Cordyceps fungus, but resources are scarce, and it is currently a hot product pursued by consumers

Method used

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  • Establishing method for cordyceps fungus fingerprints and standard fingerprints
  • Establishing method for cordyceps fungus fingerprints and standard fingerprints
  • Establishing method for cordyceps fungus fingerprints and standard fingerprints

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Embodiment Construction

[0018] 1. Method for establishing standard HPLC fingerprints of Cordyceps fungi

[0019] 1.1 Instruments and reagents

[0020] 1.1.1 Instruments

[0021] Waters 2690 HPLC high-performance liquid chromatography; photodiode array detector; Waters chromatographic workstation.

[0022] 1.1.2 Materials Reagents

[0023] Cordyceps sinensis, Cordyceps militaris, Cordyceps bassiana, Cordyceps takaomon tana, Cordyceps gunnii, Isaria farinose's stroma, hyphae Cordyceps materials such as bodies and fruiting bodies,

[0024] Standard reference substances: cordycepin, adenosine, uridine, guanosine, inosine, cytidine, thymidine, all purchased from Sigma; methanol, chromatographically pure.

[0025] 1.2 Chromatographic conditions

[0026] Chromatographic column: Agilent Zorbax Extend C18 (250mm×4.6mm, 5μm) analytical column; injection volume: 10μL; flow rate: 0.5mL / min; column temperature: 25°C; detection time: 40min; detection wavelength: 260nm. The mobile phase wipe adopts the methan...

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Abstract

The invention relates to an establishing method for HPLC fingerprints of cordyceps fungus nucleoside components in different product forms, and standard fingerprints. The employed chromatographic column is Agilent Zorbax Extend C18 (250 mm*4.6 mm, 5 mu m), the column temperature is 25 DEG C, the detection wavelength is 260 nm, the mobile phase is a methanol-water system, and a gradient elution mode is employed for obtaining of the HPLC fingerprints of nucleoside components of cordyceps stromata, mycelia and encarpia. Through comparison of the HPLC fingerprints of the stromata, mycelia and encarpia of the six cordyceps fungus, common fingerprint characteristics of the three-form cordycepses are respectively determined, and the standard fingerprints are obtained, and qualitative determination and quantitive determination are performed on common fingerprint peaks. The method of the invention has the advantages of simplicity, stability, high precision and good repeatability, and helps to accurately reflect quality state of cordyceps fungus from the integrated chromatographic characteristics of different-form cordycepses. The common fingerprint characteristics can be taken as one of the indexes for cordyceps quality control and discrimination.

Description

technical field [0001] The invention relates to a method for identifying and evaluating fungi of the genus Cordyceps, in particular to a method for identifying and evaluating fungi of the genus Cordyceps using high-performance liquid chromatography (HPLC) fingerprints, and belongs to the technical field of drug analysis. Background technique [0002] The genus Cordyceps (Cordyceps s) belongs to the phylum Fungi, the subphylum Ascomycota, the class Sclerotinia, the order Ergot, and the family Ergotaceae. The vast majority of fungi in this genus can infect insects, and grow sporosomes from their heads or body surfaces to form a complex of insects and fungi - Cordyceps. Currently, there are three main application forms of Cordyceps genus fungi: one is the original ecological Cordyceps complex (according to the definition of Chinese Pharmacopoeia and Chinese Mycology, the Cordyceps complex is composed of stroma and sclerotia. Sclerotia refers to insects covered with hyphae body...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N30/36
Inventor 江晓路沈照鹏刘养仲
Owner OCEAN UNIV OF CHINA
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