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Tibet gadol medicinal material HPLC finger print construction method and standard finger print thereof

A technology of fingerprint spectrum and construction method, which is applied in the construction of HPLC fingerprint of Rhodiola tibialis medicinal material and the field of standard fingerprint spectrum, can solve the problems of imperfect quality standards of Rhodiola tibialis, and achieves strong operability and stability. and good reproducibility and stable quality

Inactive Publication Date: 2004-11-17
SUN YAT SEN UNIV
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Problems solved by technology

[0004] Rhodiola Tibetana (Rhodiola tibetica (Hook.f.et Thomas) Fu) is a unique natural medicinal plant on the Qinghai-Tibet Plateau, but the quality standard of Rhodiola tibetan is extremely imperfect at present

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  • Tibet gadol medicinal material HPLC finger print construction method and standard finger print thereof

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Embodiment Construction

[0014] Preferred embodiments of the present invention are as follows:

[0015] 1. Instruments and reagents

[0016] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph; Chromatographic column: hypersil ODS (5um, 250×4.0mm).

[0017] 1.2 Test drug: There are 20 batches of Rhodiola tibetan medicinal materials, all of which come from the Tibet Autonomous Region. After identification, they are all Rhodiola tibetica (Rhodiola tibetica (Hook.f.et Thomas) Fu), a plant of the genus Rhodiola in the family Crassulaceae. All reagents used in the experiment were of analytical grade.

[0018] 2. Methods and Results

[0019] 2.1 Preparation of the test solution: Accurately weigh 100g of each batch of Rhodiola rosea powder, add 6L of 85% ethanol to each batch, extract with MCL-3 continuous microwave reactor for 30 minutes, add dropwise to the extract 10% (W / V) lead acetate solution 200ml produces precipitation, centrifuges, and the obtained supernatant is used as the test s...

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Abstract

The invention refers to a constructing method for HPLC fingerprint diagram of Rhodiola tibetica (Hook.f.et Thomas) Fu medicinal materials and the standard fingerprint diagram. At first, takes the Rhodiola tibetica (Hook.f.et Thomas) Fu powder, adds in alcohol, carries on microwave extraction, the lead acetate liquid is added into the extraction liquid and generates deposit, it is centrifugated, the acquired supernate is used as testing liquid; then, carries on high efficiency liquid phase color spectrum analysis: absorbs the testing sample liquid, the color spectrum pole is hypersil ODS (5m, 2504.0mm), the flow phase is 0.05% phosphoric acid buffer solution-methyl alcohol, the invention uses ladder eluting method; the testing wavelength: 360nm; acquires the fingerprint diagram of HPLC, confirms the fingerprint character, acquires the standard fingerprint diagram of the medicine. The HPLC fingerprint character can monitor the quality of Rhodiola tibetica (Hook.f.et Thomas) Fu, ensures it is stable, even, and controllable.

Description

technical field [0001] The invention relates to a method for constructing an HPLC fingerprint of a medicinal material Rhodiola tibetica (Hook.f.et Thomas) Fu, and a standard fingerprint of a medicinal material of Rhodiola tibetan obtained by the method. Background technique [0002] Chromatographic fingerprint quality control technology is a bright spot in the current quality research of traditional Chinese medicine. For traditional Chinese medicine with complex ingredients, the past practice of using a single active ingredient as its quality control index is not comprehensive, and the analogy of "blind men feeling the elephant" cannot be more appropriate. The chromatographic fingerprint quality control mode uses chromatographic analysis as a means to obtain information reflecting the internal quality of traditional Chinese medicines, and evaluates the authenticity, consistency and stability of the internal quality of traditional Chinese medicines through the analysis and co...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 苏薇薇葛发欢王永刚潘隽丽宋晓虹方铁铮吴忠
Owner SUN YAT SEN UNIV
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