Fingerprint detection method of cyclomastopathy eliminating pill preparation

Abstract

The invention discloses an HPLC fingerprint detection method of a cyclomastopathy eliminating pill preparation. The method comprises the following steps: preparing a test substance solution, preparing a reference substance solution, and determining the correlation between all bulk drugs and a finished product; and determining by using a high performance liquid chromatograph, calculating the relative retention time and the relative peak area of the test substance with the retention time and the peak area of a reference peak as 1 to obtain the HPLC fingerprint of the cyclomastopathy eliminating pill preparation. The HPLC fingerprint of athe cyclomastopathy eliminating pill preparation obtained through the method shows the numbers of characteristic peaks of radix bupleuri, Rhizoma Cyperi, Rheum officinale, Ligusticum wallichii, Rhizoma Curcumae, Chinese angelica, white peony root, Fritillaria thunbergii Miq., Vaccaria segetalis and green tangerine peel below 254nm are 4, 4, 17, 7, 5, 7, 11, 4, 6 and 11 respectively. Experiment verification results show that the fingerprint detection method has the advantages of high sensitivity and precision, good stability and repeatability, high similarity, objective, comprehensive and accurate evaluation of the quality of the cyclomastopathy eliminating pill preparation, and importance for guaranteeing of the clinic drug effect.

Description

[0001] priority [0002] For this application, the applicant claims the priority of the patent filed in China with the application date of July 30, 2013 and the application number 201310325229.8. technical field [0003] The invention relates to a quality detection method of traditional Chinese medicine preparations, in particular to a fingerprint detection method of Xiaozheng pill preparations. Background technique [0004] Xiaozheng Pills is made by Mr. Zhao Junxue, an old Chinese medicine doctor of Henan College of Traditional Chinese Medicine, on the basis of Bupleurum Shugan Powder in Zhang Jiebin's "Jingyue Quanshu" in Ming Dynasty, and according to the pathogenesis of hyperplasia of mammary glands. It has been used in clinic for decades. The curative effect on mammary gland hyperplasia is definite. The prescription is composed of bupleurum, rhubarb, rhubarb, green skin, chuanxiong, angelica, white peony root, curcuma, wood beetle, fritillary fritillaria, and wangbuli...

AI Technical Summary

Problems solved by technology

The steps of this method are cumbersome, and the preparation of Xiaozheng Pill is composed of eleven medicinal materials, and the determination of the content of only one medicinal material is not enough to comprehensively control the quality of the compound preparation

The existing methods do not conduct a comprehensive and systematic detection of the chemical components in the main multi-flavored medicinal materials, so the quality of intermediates and finished products cannot be fully monitored

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