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Identification method of propolis medicinal materials and preliminary classification method of propolis medicinal material growing district

A technology of propolis and medicinal materials, applied in material separation, medical formula, analysis of materials, etc., can solve the problems of difficult implementation of propolis quality control, affecting the quality of life, propolis export earning foreign exchange, and inability to distinguish gum from propolis, etc. It is easy to master and the method is simple , good reproducibility

Active Publication Date: 2008-12-24
广州市宝生园股份有限公司 +1
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Problems solved by technology

However, because propolis is derived from gum and its composition is similar to gum, the current pharmacopoeia methods and industry standards cannot distinguish gum from propolis, making it difficult to implement propolis quality control, which has significantly affected the quality of life of people and the export of propolis foreign exchange earning

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  • Identification method of propolis medicinal materials and preliminary classification method of propolis medicinal material growing district
  • Identification method of propolis medicinal materials and preliminary classification method of propolis medicinal material growing district
  • Identification method of propolis medicinal materials and preliminary classification method of propolis medicinal material growing district

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Embodiment Construction

[0050] Preferred embodiments of the present invention are as follows:

[0051] 1. Instruments, reagents and materials

[0052] 1.1 Instruments and materials: German Dionex P680A high performance liquid chromatography (PDA-100 detector, STH585 column thermostat, P680HPLC pump, ASI-100 autosampler), chromeleon color Pu workstation; 8953Dietikon electronic analytical balance (Switzerland , accuracy 0.1mg); AS2060B ultrasonic instrument (Tianjin Autosaiens Instrument Co., Ltd.), etc.

[0053] 1.2 Reagents: Rutin reference substance (080-9705), quercetin reference substance (081-9003), chrysin (111701-200501), galangin (111699-200501) are all provided by China Pharmaceutical and Biological Products Identification Institute , propolis (hair glue, provided by Guangzhou Baoshengyuan Co., Ltd., identified by Lu Zhanlie, Guangzhou Bee Products Research Institute, is propolis). Methanol used in the mobile phase is chromatographic alcohol, and other reagents such as methanol and phospho...

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Abstract

The invention relates to a propolis (as a medicinal material) distinguishing method and a preliminary classification method of propolis production areas. First test sample solution is prepared, then control solution is prepared. The measurement method is as follows: 10mul of the reference solution and 10mul of the test sample solution are respectively and accurately extracted and then injected into a high performance liquid chromatograph; the test is performed according to the high performance liquid chromatograph; with the control solution as the reference, the chromatogram is measured and recorded; then a propolis HPLC finger-print map is established. The invention has the advantages of convenient method, stability, high accuracy, good reproduction and easiness in mastering, and also has the advantages that the trueness of the propolis can be mastered from the whole characteristic appearance of the chromatogram, and the propolis distinguishing method can be used as one of the indexes for controlling the propolis quality and distinguishing the trueness of the propolis. Meanwhile the propolis from different production areas also undergoes preliminary classification.

Description

technical field [0001] The invention relates to an identification method of propolis medicinal material and a preliminary classification method of propolis medicinal material production area, in particular to a method for identifying propolis medicinal material by using a patterned high-performance liquid chromatography (HPLC) fingerprint. Background technique [0002] Propolis (propolis) is a gum collected by bees from plant buds and trunks, mixed with bee mandibular gland secretions and beeswax to form a colloidal solid with aromatic flavor. The chemical composition of propolis is related to the bee species and the vegetation in the surrounding areas where the bees live. There are hundreds of known chemical compositions of propolis. The one-sidedness of the material group should be controlled as a whole. Therefore, in addition to "micro analysis", a certain "macro analysis" method is also applied to effectively characterize the quality of traditional Chinese medicine as a...

Claims

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Application Information

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IPC IPC(8): A61K35/64G01N30/02G01N30/86G06F19/00A61K35/644
Inventor 林励王小平郑尧隆潘建国卢占列
Owner 广州市宝生园股份有限公司
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