HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria

A high-performance liquid chromatography and Uncaria technology, applied in the field of analysis of Chinese herbal medicines, can solve problems such as the difficulty in distinguishing the components of Uncaria, affecting the identification fingerprints of Uncaria species, and achieve the effect of reducing mutual interference and effective separation

Active Publication Date: 2014-06-18
GUANGDONG PHARMA UNIV
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Problems solved by technology

At present, dozens of Uncaria alkaloid components have been found in the main Uncaria species in my country. Although the components contained in different Uncaria species are mostly different, because Uncaria alkaloids are all indole alkaloids, the parent structure is similar, and there are relatively Many homologues and isomers, some of which are stereoisomers, are separated by HPLC technology with a separation effect much higher than that of TLC, and it is also quite difficult to distinguish the components of different varieties of Uncaria, which affects the subsequent identification of Uncaria species and their Fingerprint creation

Method used

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  • HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria
  • HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria
  • HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria

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Effect test

Embodiment 1

[0062] Using the method of the above step 1, the sources of preparation are respectively Dapu County, Meizhou, Tianzhu County, Guizhou, Meizhou City, Guangdong, Guiyang City, Guizhou, Yangshan County, Shaoguan, Wuzhou, Guangxi, Nanning City, Guangxi, and genuine Uncaria uncaria in Jiangxi (all certified Character identification by plant identification experts, and identified by DNA molecular identification technology) 7 parts of stem hook detection solution, using the method of step 2 for high performance liquid chromatography detection, the chromatogram is as follows figure 1 shown.

Embodiment 2

[0064] Using the method of the above step 1, the sources of preparation are Dinghu Mountain in Zhaoqing, Guangdong, Guangxi Botanical Garden, and Uncaria macrophylla emerging in Yunfu, Guangdong (all identified by plant identification experts and identified by DNA molecular identification technology) stem hook detection solution 3 Parts, using the method of step 2 to carry out high performance liquid chromatography detection, the chromatogram is as follows figure 2 shown.

Embodiment 3

[0066] Using the method of the above step 1, 3 parts of detection solutions were prepared from the stems and leaves of Uncaria tomentosa in Dinghushan, Guangdong, and Uncaria tomentosa in Nanning, Guangxi (both were identified by plant identification experts and identified by DNA molecular identification technology) , using the method of step 2 to carry out high performance liquid chromatography detection, the chromatogram is as follows image 3 shown.

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Abstract

The invention belongs to the field of analysis of traditional Chinese medicinal materials, and particularly discloses an HPLC (High performance liquid chromatography) method for distinguishing different cultivars of uncaria. All chromatographic peaks are separated effectively when the uncaria traditional Chinese medicinal materials are subjected to HPLC detection by optimizing each experimental parameter; the phenomena of mutual interference of the chromatographic peaks, trailing and the like are reduced; mutual interference of the chromatographic peaks of different ingredients in different cultivars of uncaria in HPLC detection is basically avoided, so as to provide basis for authenticating different cultivars of uncaria and constructing a uncaria HPLC fingerprint spectrum.

Description

technical field [0001] The invention belongs to the field of analysis of traditional Chinese medicinal materials, and in particular relates to a high performance liquid chromatography detection method for distinguishing different species of Uncaria. Background technique [0002] Uncaria is a commonly used traditional Chinese medicine in Chinese medicine. It is slightly cold in nature and sweet in taste. It returns to the liver and pericardium meridians. It can clear away heat and calm the liver, calm the wind and calm convulsions. [0003] Uncaria has many chemical components, and different varieties of Uncaria have different chemical components. The main chemical components contained in Uncaria are indole alkaloids, triterpenoids, flavonoids, coumarins, quinoxacid saponins, phenols and organic acids, etc., and the main effective chemical components of Uncaria are currently Alkaloid ingredients. [0004] The 2010 edition of "Chinese Pharmacopoeia" stipulates that Uncaria ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 曾常青黄样增陈其钊
Owner GUANGDONG PHARMA UNIV
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