Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof

A technique for fingerprinting and establishing methods, which is applied in the field of establishing methods for HPLC fingerprints of curcuma officinalis medicinal materials and its fingerprints, can solve problems such as research on other quality aspects that have not been seen, and achieve chromatographic conditions that are easy to implement, chromatographic peak information is abundant, and preparation simple effect

Active Publication Date: 2016-07-06
GUILIN EIGHT PLUS ONE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Scholars have studied the pharmacological effects of Curcuma officinalis and the GC-MS analysis of volatile oil components in Curcuma officinalis, but there are no other studies on quality

Method used

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  • Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof
  • Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof
  • Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the method establishment and verification of the curcuma officinalis medicinal material HPLC fingerprint

[0047] 1. Instruments and Materials

[0048] 1.1 Instruments: Diana P680 high performance liquid chromatography, including P680 infusion pump, ASI-100 autosampler, column thermostat and ultraviolet detector, chromeleon chromatography workstation. BK-240A type ultrasonic cleaning machine (Barker Ultrasonic Technology Group Co., Ltd.), BP211 type electronic analytical balance (Germany Sartorius).

[0049] 1.2 Reference substances and reagents: Curcumadione reference substance (Chengdu Pufeide Biotechnology Co., Ltd., batch number 130713), curcumadiol reference substance, and original curcumol reference substance are self-made, and all reference substances have a purity greater than 98%. Acetonitrile (U.S. Fisher Chemical Reagent Company, chromatographically pure), phosphoric acid (Tianjin Concord Technology Co., Ltd., analytically pure), methanol (Tian...

Embodiment 2

[0063] Example 2: Detection and data analysis of 10 batches of curcuma officinalis fingerprints

[0064] 1.1 Determination of fingerprints of Curcuma officinalis medicinal materials: 10 batches of curcuma officinalis medicinal materials were taken and measured according to the conditions in Example 1, and the HPLC spectra of 10 batches of samples were obtained. No. 1 peak (curcumadiol) is used as a reference, and 12 common peaks have been determined, such as figure 1 . Calculate the relative retention time and relative peak area of ​​each common peak, and the RSD of the relative retention time of each common peak is <0.15%, but the RSD of each relative peak area is relatively large, indicating that the content of medicinal materials in different origins is quite different , the results are shown in Table 2-3.

[0065] The relative retention time of table 210 batches of medicinal materials HPLC fingerprint

[0066]

[0067] Table 310 batches of medicinal materials HPLC fi...

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Abstract

The invention discloses an establishment method of a curcuma aromatica medicine fingerprint map and the fingerprint map thereof, and particularly, provides establishment of a HPLC fingerprint map for controlling the quality of curcuma aromatica medicine. The method includes the steps of: 1) preparation of a reference substance solution: preparing the reference substance solutions of curdione, curcumadiol, procurcumenol; 2) preparation of a sample solution: extracting the curcuma aromatica medicine and filtering an extract liquid; 3) HPLC detection to obtain the fingerprint map: performing HPLC detection with octadecylsilane chemically bonded silica as a filler and an acetonitrile-tetrahydrofuran-0.1% phosphoric acid water system as a mobile phase in a chromatographic column in a gradient elution manner, wherein column temperature is 30 DEG C, ultraviolet detection wavelength is 230-250 nm and time is 60-65 min; and 4) similarity evaluation: introducing the obtained fingerprint map into "fingerprint similarity evaluation system of traditional Chinese medicine chromatography" (2004A version) similarity software for performing similarity evaluation. The method is simple, quick and accurate, has good repeatability, and provides powerful guarantee for comprehensive and effective control on quality of the curcuma aromatica medicine.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a quality control method of traditional Chinese medicinal materials, in particular to a method for establishing HPLC fingerprints of curcuma curcuma and the fingerprints thereof. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. It can more comprehensively reflect the types and quantities of chemical components contained in medicinal materials, and effectively reflect the integrity and comprehensive effects of traditional Chinese medicine ingredients. It has been widely used in the analysis, identification and quality control of traditional Chinese medicine due to its fast and accurate characteristics. [0003] Curcuma aromatica Salisb. is the dry rhizome o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张恩德黄景华王习著臧成旭
Owner GUILIN EIGHT PLUS ONE PHARMA CO LTD
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