Method for detecting content of various kinds of ingredients in herba gerberae piloselloidis medicinal material

The technology of Gerbera vulgaris and herbal materials is applied in the field of content determination of various components in Gerbera vulgaris, which can solve the problems of content determination of beneficial components, imperfect quality standards of Gerbera vulgaris, and inability to comprehensively control the internal quality of Gerbera vulgaris. , to achieve the effect of simple method and good repeatability

Inactive Publication Date: 2018-09-04
GUIZHOU MEDICAL UNIV
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Problems solved by technology

Rhizoma Rhododendron was included in the 2003 edition of "Quality Standards for Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province" and the 2005 edition of "Quality Standards for Decoction Pieces of Tra...

Method used

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  • Method for detecting content of various kinds of ingredients in herba gerberae piloselloidis medicinal material
  • Method for detecting content of various kinds of ingredients in herba gerberae piloselloidis medicinal material
  • Method for detecting content of various kinds of ingredients in herba gerberae piloselloidis medicinal material

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Embodiment

[0036] Instruments and materials:

[0037] Acquity UPLC-PDA system (Waters company, including binary ultra-high pressure gradient pump, autosampler, chromatographic column thermostat, array diode detector, Empower workstation); EL204 1 / 10000 electronic balance (Mettler- Toledo Instruments Shanghai Co., Ltd.); ultrapure water machine (Sichuan Votel Technology Development Co., Ltd.); DK-98-Ⅱ electric constant temperature water bath (Tianjin Test Instrument Co., Ltd.).

[0038]Arbutin reference substance (batch number: 111951-201301), chlorogenic acid (batch number: 110753) and luteolin (batch number: 111720-201609) were purchased from China National Institutes for Food and Drug Control; isochlorogenic acid A (batch number: MUST-16031611), isochlorogenic acid B (lot number: MUST-16031612) and isochlorogenic acid C (lot number: MUST-16031613) were purchased from Chengdu Master Biotechnology Co., Ltd. / Chengdu Institute of Biology, Chinese Academy of Sciences.

[0039] Methanol (an...

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Abstract

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for detecting the content of various kinds of ingredients in herba gerberae piloselloidis medicinal material. The method adopts a UPLC-PDA method, takes Waters BET C18 (2.1*150mm, 1.7microns) as a chromatographic column, acetonitrile-0.1% formic acid water solution is subjected to gradient elution, the detection wavelength is 280nm and 328nm, the flowing velocity is 0.28L/min, and the column temperature is 40 DEG C. According to the method, chlorogenic acid, isochlorogenic acid A,isochlorogenic acid B, isochlorogenic acid C, galuteolin and arbutin in herba gerberae piloselloidis medicinal material can be well separated, the average recovery of the method is 97.20%-103.57%, andRSD (relative standard deviation) is less than or equal to 3.0%. The method is simple and convenient and precise, the repeatability is good, the method can be used for detecting the content of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, galuteolin and arbutin in herba gerberae piloselloidis medicinal material, and reference foundations are provided for quality control of herba gerberae piloselloidis.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicines, in particular to a method for measuring the contents of various components in the herba basilica medicinal material. Background technique [0002] Gerbera japonicus is the dry whole herb of Piloselloides hirsuta hirsuta (Forsk.) G. Jeffrey of Compositae, and it is also used by ethnic minorities in Guizhou Province. Rhododendron is mild in nature, bitter and pungent, and returns to the lung and stomach meridians. It has the effects of clearing the lung and relieving cough, producing sweat and diuresis, promoting qi and blood circulation, and is mainly used for cold cough, asthma, edema, fullness of urination, dyspepsia in children, amenorrhea, Injuries from falls, carbuncles, furuncles and other diseases. According to the "Guiyang Folk Medicinal Herbs", gerbera is mainly used to treat lung-related diseases such as wind cough, cough asthma and lung abscess. Mode...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘春花孙佳陆苑潘洁李勇军马雪李月婷陈思颖王爱民王永林
Owner GUIZHOU MEDICAL UNIV
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