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Method for extracting chlorogenic acid and isochlorogenic acid from gynura procumbens

A technology of flat-lying chrysanthemum notoginseng and isochlorogenic acid, which is applied in the field of extracting chlorogenic acid and isochlorogenic acid, can solve the problem of not being able to meet the market demand for high-purity chlorogenic acid, not suitable for large-scale industrial production, and restricting pharmacological drugs Effective in-depth research and other issues, to achieve good market prospects, low cost, high production safety effect

Active Publication Date: 2018-10-23
江西省蔓三七健康科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When these methods are used alone, the total yield of chlorogenic acid is about 2.5%, and the purity is below 40%, and it is difficult to achieve a purity of more than 95%.
At present, the production of chlorogenic acid mainly has the following problems: the method capable of extracting high-purity chlorogenic acid is not suitable for industrialized large-scale production, and the purity of chlorogenic acid obtained by the method suitable for industrialized large-scale production is low, which cannot meet Market demand for high-purity chlorogenic acid
Isochlorogenic acid has good pharmacological activity, but it is difficult to prepare high-purity monomers, which restricts the in-depth study of its pharmacological effects
[0005] At present, in the research on chlorogenic acid and isochlorogenic acid in the leaves of Panax notoginseng, most researchers have obtained the extract of chlorogenic acid from Panax notoginseng, and the isochlorogenic acid mixed in the extract of chlorogenic acid , without effectively separating them

Method used

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  • Method for extracting chlorogenic acid and isochlorogenic acid from gynura procumbens
  • Method for extracting chlorogenic acid and isochlorogenic acid from gynura procumbens
  • Method for extracting chlorogenic acid and isochlorogenic acid from gynura procumbens

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Experimental program
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Effect test

Embodiment 1

[0037] Firstly, the modified silica microspheres are prepared for future use; the micron-sized silica microspheres for preparing the modified silica microspheres are commercially available known raw materials.

[0038] A preparation method of modified silicon dioxide microspheres, which comprises the following specific steps:

[0039] (a) 5 g of micron-sized silica microspheres with a particle size of 1 μm were vacuum-dried at 130°C for 3 hours, and then treated with 0.5 g of 30% sodium hydroxide to activate the surface active group silanol, Then rinse with pure water until neutral;

[0040] (b), add 3ml of ethyl chloroacetate to the solution washed to neutral in step a, mix and stir in the 70% volume concentration ethanol solution for 2h, continue to add 3ml of ethylenediamine, react at room temperature for 5h, and put The solution was centrifuged to collect the product, and then washed with dry dichloromethane at a dosage of 5ml each time for 3 times to remove the remaining...

Embodiment 2

[0051] First prepare modified silica microspheres for subsequent use; prepare micron-sized silica microspheres of modified silica microspheres to be synthesized using the improved Stöber method, which includes the following specific steps:

[0052] Step a1. Dissolve 0.03g KCl in 10 g deionized water, transfer the KCl solution to a 250 mL three-neck flask, add 75 g absolute ethanol, 4 mL NH 3 •H 2 O and 2g tetraethyl orthosilicate, mix and stir until the solution is milky white;

[0053] Step a2. Disperse 4g of tetraethyl orthosilicate in 25g of absolute ethanol. The mixed solution obtained after ultrasonic dispersion is added dropwise at room temperature at a rate of 0.1 mL / min to the milky white solution in the three-necked flask in step a1. 3 After the addition of h is completed, the reaction is continued for 1 h, and after the reaction is completed, the product is collected;

[0054] In step a3, the obtained product is washed with water and ethanol several times until the...

Embodiment 3

[0068] First prepare modified silica microspheres for subsequent use; prepare micron-sized silica microspheres of modified silica microspheres to be synthesized using the improved Stöber method, which includes the following specific steps:

[0069] Step a1. Dissolve 0.06g KCl in 15 g deionized water, transfer the KCl solution to a 250 mL three-neck flask, add 80 g absolute ethanol, 7 mL NH 3 •H 2 O and 5g ethyl orthosilicate, mix and stir until the solution is milky white;

[0070] In step a2, 8 g of tetraethyl orthosilicate is dispersed in 33 g of absolute ethanol, and the mixed solution obtained after ultrasonic dispersion is added dropwise at room temperature at a speed of 0.3 mL / min to the milky white solution in the three-necked flask in step a1, 3 After the addition of h is completed, the reaction is continued for 1 h, and after the reaction is completed, the product is collected;

[0071] In step a3, the obtained product is washed with water and ethanol several times ...

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Abstract

The invention discloses a method for extracting chlorogenic acid and isochlorogenic acid from gynura procumbens. The method comprises the following steps of drying and smashing fresh gynura procumbensleaves, and obtaining powder; mixing the powder and an ethanol solution, adopting ultrasonic extraction to obtain an extracting solution, and then centrifuging, filtering and concentrating to obtaina turbid dark green concentrated solution; adding a clarifying agent into the concentrated solution for clarifying; adding modified silicon dioxide pellets into the clarified concentrated solution, de-coloring, and enabling the color of the concentrated solution to change into light gray from dark green; adding absolute ethyl alcohol into the light-gray concentrated solution, and eliminating carbohydrates; adsorbing the carbohydrates-eliminated through a middle0high-pressure chromatographic column; after completely adsorbing, desorbing in a classification way, concentrating, and drying to obtain a chlorogenic acid finished product dry powder and isochlorogenic acid mixture. The method provided by the invention is simple in process, convenient to operate, low in cost, free of harmful solvent, high in product purity, better in purification and impurity cleaning effects, energy saving, environmental-friendly, high in production safety, and suitable for industrial production, and has a favorable market prospect.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting chlorogenic acid and isochlorogenic acid from chrysanthemum notoginseng. Background technique [0002] Pingwo chrysanthemum notoginseng, also known as vine notoginseng, snake joint bone, continual life grass, fairy grass, has a pungent, slightly bitter, cool nature, and is a perennial herb with dual purposes for medicine and food. Folks use it to dredge meridian and activate collaterals, reduce swelling and relieve pain, reduce inflammation and relieve cough, treat bruises, bronchial pneumonia, tuberculosis, etc. Modern biochemical and medical research has proved that Panax notoginseng has both nutritional value and pharmacological effects on the human body. In the first half of 2009, the Chinese Center for Disease Control and Prevention, the National Center for Food Quality Supervision and Inspection, and the China Research Institute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56C07C67/31C07C69/675C07C231/02C07C235/10B01J20/22B01J20/28B01J20/30
CPCB01J20/10B01J20/22B01J20/28021C07B2200/11C07C67/31C07C67/48C07C67/56C07C231/02C07C2601/14C07C69/732C07C69/675C07C235/10
Inventor 胡居吾韩晓丹吴磊涂招秀谢欣黄斌华
Owner 江西省蔓三七健康科技有限公司
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