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Method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection

The technology of a traditional Chinese medicine preparation and a determination method is applied in the field of content determination of various components in Shuanghuanglian, which can solve the problems of occupying a large number of personnel, equipment, high detection cost, and inability to fully reflect the integrity of fingerprints, and achieve the effect of shortening the inspection period.

Active Publication Date: 2013-09-18
哈药集团中药有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that a sample needs to be processed by different methods and tested under different conditions in the existing Shuanghuanglian quality inspection process for injection. Part of the sample processing method is complicated and has disadvantages, which cannot fully reflect the integrity of the fingerprint. Due to the problems of high cost, a large amount of waste liquid, a large number of personnel and equipment, and a long inspection cycle, a method for the determination of various components in Shuanghuanglian for injection of traditional Chinese medicine preparations is provided.

Method used

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  • Method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection
  • Method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection
  • Method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection

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specific Embodiment approach 1

[0028] Embodiment 1: A method for determining the content of various components in Shuanghuanglian for injection of a traditional Chinese medicine preparation according to the present embodiment is carried out according to the following steps:

[0029] 1. Preparation of the baicalin reference substance: take the baicalin reference substance, add buffered saline solution to make a solution with a concentration of 0.5 mg / mL, and use it as the baicalin reference substance;

[0030] 2. Preparation of mixed reference substance solution: Weigh the reference substance, add buffered saline solution to make each 1mL containing 10μg neochlorogenic acid, 40μg chlorogenic acid, 8.0μg cryptochlorogenic acid, 4.0μg isochlorogenic acid A, 10.0 μg of isochlorogenic acid C, 8.0 μg of forsythin, 20 μg of scutellarin-7-O-glucuronide, 3.0 μg of wogonin, 2.0 μg of baicalein and 0.6 μg The solution of wogonin is used as a mixed reference substance;

[0031] 3. Preparation of the test solution: tak...

specific Embodiment approach 2

[0034] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the multi-wavelength simultaneous monitoring or wavelength monitoring over time refers to the neochlorogenic acid and chlorogenic acid in the test sample of Shuanghuanglian for injection of Chinese medicine preparations Acid, Cryptochlorogenic Acid, Caffeic Acid, Forsythiaside A, Isochlorogenic Acid A, Isochlorogenic Acid C, Melaleucain-7-O Glucuronide, Wogonin, Baicalein and Chinese Scutellariae luteolin and rutin were detected at 350nm, forsythin was detected at 208nm or 230nm; baicalin was detected at 240nm or 350nm. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the buffered saline solution is phosphate buffer with pH=5.0-6.0, acetic acid-sodium acetate buffer with pH=4.5, phosphate Buffer mixed solution or acetic acid-sodium acetate buffer mixed solution; wherein, the phosphate buffer mixed solution is added to methanol or acetonitrile by adding phosphate buffer solution with pH=5.0 to 6.0 to make the volume percentage 5% to 50% of the mixed solution (the volume percentage refers to the volume of the phosphate buffer solution in the mixed solution); the acetic acid-sodium acetate buffer solution is composed of acetic acid-sodium acetate with pH=4.5 The buffer solution is added to methanol or acetonitrile to prepare a mixed solution with a volume percentage of 5-50% (the volume percentage refers to the volume of the acetic acid-sodium acetate buffer solution in the mixed solution). Others are the same as in the first or ...

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Abstract

The invention relates to a method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection, aiming at solving the problems of an existing method for content determination of Shuanghuanglian for injection which is complex in sample processing, time, labor and detection equipment-consuming to detect Shuanghuanglian, and long in detection period. The detection method provided by the invention can be used for synchronously determining the contents of more than 10 components such as forsythin, forsythiaside A, caffeic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C, baicalin, scutellarin and oroxylin-7-O-glucuronic acid in the Shuanghuanglian for injection, and meanwhile monitoring a fingerprint spectrum. The method can be used for completing previous detection in one time just by one detection system, and is more accurate, stable, comprehensive and rapid in control over the quality of the Shuanghuanglian for injection.

Description

technical field [0001] The invention relates to a method for determining the content of various components in Shuanghuanglian. Background technique [0002] Shuanghuanglian for injection is a natural drug injection made from the extracts of honeysuckle, scutellaria baicalensis and forsythia. Its main components are phillyrin, rutin, baicalin, OroxylinA-7-O-glucuronide, wogonin ( Wogonoside), baicalein, wogonin, Forsythoside A, 5-Caffeoylquinic acid, 3-Caffeoylquinic acid, cryptochlorogenic acid (4-Caffeoylquinic acid), isochlorogenic acid A (3,5-Dicaffeoylquinic acid), isochlorogenic acid B (3,4-Dicaffeoylquinic acid), isochlorogenic acid C (4,5-Dicaffeoylquinic acid), etc. It has antiviral and antibacterial dual therapeutic effects, and has obvious effects on more than 20 common viruses such as influenza. Its antibacterial effect is different from other commonly used anti-infective drugs, especially for the treatment of antibiotic-resistant bacterial infections. Judging ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孙永慧李文春张丽君崔仁海解黎雯李长新付饶单钰毓裴福成苏德龙王英新颜莉
Owner 哈药集团中药有限公司
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