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Industrial method for synchronously preparing stevia rebaudiana chlorogenic acid and stevioside

A technology of stevioside and chlorogenic acid, which is applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc. The problems of reduced isochlorogenic acid content in leaf chrysanthemum and poor product quality stability can achieve the effect of effective separation, reduction of sewage and flocculation residue discharge, and improvement of production efficiency

Pending Publication Date: 2019-03-08
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] (1) In the traditional water extraction process, the isochlorogenic acid in stevia is hydrolyzed, and this process is affected by various factors such as enzymes and temperature. The process is not easy to control, the content of stevia isochlorogenic acid is reduced, and the product quality stability is poor
[0012] (2) The polarity of steviol glycosides and stevia chlorogenic acid is close to the separation difficulty

Method used

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  • Industrial method for synchronously preparing stevia rebaudiana chlorogenic acid and stevioside
  • Industrial method for synchronously preparing stevia rebaudiana chlorogenic acid and stevioside
  • Industrial method for synchronously preparing stevia rebaudiana chlorogenic acid and stevioside

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Effect test

Embodiment 1

[0049] The present invention provides an industrial method for preparing stevia chlorogenic acid and the resulting product, the specific steps are as follows:

[0050] (1) Weigh 1 kg of Stevia rebaudiana powder, 85% ethanol aqueous solution as the extraction solution, the ratio of solid to liquid is 1:5 / 3.5 / 3.5, extract three times at 50°C, the first extraction time is 1.5h, 2 and 3 times The extraction time was 1 h, and the combined filtrate was used as the extract.

[0051] (2) The extract was concentrated 10 times in a water bath at 60°C and vacuum -0.08MPa to recover ethanol.

[0052] (3) Use a potentiometric titrator for on-line monitoring, use H 3 PO 4 The aqueous solution adjusts the state of the feed liquid, and the stirring is continued during the process, and the liquid addition is stopped when the electrode potential suddenly jumps.

[0053] (4) Use an equal volume of chloroform to extract the feed liquid obtained in the previous process step 3 times. After the o...

Embodiment 2

[0055] The present invention provides an industrial method for preparing stevia chlorogenic acid and the resulting product, the specific steps are as follows:

[0056] (1) Weigh 1 kg of Stevia rebaudiana powder, 95% methanol aqueous solution is the extraction solution, the ratio of solid to liquid is 1:6 / 4.5, extract twice at 50°C, the first extraction time is 1.5h, and the second extraction time Both were 1h, and the combined filtrate was used as the extract.

[0057] (2) The extract was concentrated 10 times in a water bath at 60°C and vacuum -0.08MPa to recover ethanol.

[0058] (3) Use PHS-3C pH meter for online monitoring, use H 2 SO 4 The aqueous solution adjusts the state of the feed liquid, and the process is continuously stirred until the electrode potential is 180mV to stop adding liquid.

[0059] (4) Using an equal volume of ethyl acetate to extract the feed liquid obtained in the previous process step 3 times, after the organic phase was concentrated and the res...

Embodiment 3

[0061] The present invention provides an industrial method for preparing stevia chlorogenic acid and the resulting product, the specific steps are as follows:

[0062] (1) Weigh 1 kg of Stevia rebaudiana powder, 75% propanol aqueous solution is the extraction solution, the ratio of solid to liquid is 1:6 / 4.5, extract twice at 50°C, the first extraction time is 1.5h, and the second extraction The time is 1h, and the combined filtrate is used as the extract.

[0063] (2) The extract was concentrated 10 times in a water bath at 60°C and vacuum -0.08MPa to recover ethanol.

[0064] (3) Use a PHS-3C pH meter for online monitoring, use formic acid aqueous solution, and keep stirring during the process, and stop adding liquid when the pH is 3.0.

[0065] (4) Use an equal volume of ether to extract the feed liquid obtained in the previous process step 3 times. After the organic phase is concentrated and the resin is purified, 72.8g of stevia chlorogenic acid product is obtained. The ...

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Abstract

The invention relates to an industrial method for synchronously preparing stevia rebaudiana chlorogenic acid and stevioside. The industrial method includes carrying out alcohol extraction on stevia rebaudiana which is used as a raw material, and then adjusting feed liquid states to allow chlorogenic acid to be in free molecular states; carrying out extraction separation by water-insoluble moderate-polarity organic solvents; enriching the stevia rebaudiana chlorogenic acid on organic layers; enriching the stevioside on water layers. Compared with the traditional water extraction processes, theindustrial method has the advantages that chlorogenic acid components in the stevia rebaudiana can be prevented from being hydrolyzed, so that the contents and effects of effective components in stevia rebaudiana chlorogenic acid products can be guaranteed; effective separation can be carried out on the premise that the quality and the production efficiency of stevioside products are unaffected, the production efficiency can be greatly improved, and the proportion of isochlorogenic acid in the obtained products is close to the proportion of isochlorogenic acid in the raw material; production water consumption can be reduced to a great extent, discharge of sewage and flocculation residues can be decreased, accordingly, the industrial method is a green production process with high benefits,and the progress of the industry can be promoted to a great extent.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and relates to an industrial method for synchronously and efficiently preparing stevia chlorogenic acid and steviol glycosides. Background technique [0002] Stevia rebaudiana (Stevia rebaudiana) is a perennial herb of Compositae, native to Paraguay and Brazil in South America. It is one of the known sugar plants with high sweetness and has become the third natural sugar after sucrose and beet sugar. source. At present, China is the world's largest producer and supplier of steviol glycosides, accounting for more than 80% of the global total. Stevia rebaudiana contains high content of phenols besides steviol glycosides, and these phenols have important biological activities. It has been used as sweet tea and herbal tea for more than 100 years in its origin. [0003] Steviol glycoside is the sweet component in stevia rebaudiana, which is a kaurene diterpene glycoside substance. It contains...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/58C07C67/56C07C69/732C07H15/256C07H1/08
CPCC07C67/48C07C67/56C07H1/08C07H15/256C07C69/732C07C67/58C07C2601/16C07C2601/14
Inventor 连运河徐美利高伟牛志平王艳芳
Owner CHENGUANG BIOTECH GRP CO LTD
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