Diabetes treating traditional Chinese medicine preparation identification method

Abstract

The present invention discloses a diabetes treating traditional Chinese medicine preparation identification method. According to the present invention, the traditional Chinese medicine preparation is prepared from Keyaoluoqu, plantago depressa willd, agrimonia pilosa ledeb, and lonicera confusa dc; the identification is carrying out thin-layer chromatography on Keyaoluoqu, plantago depressa willd, agrimonia pilosa ledeb, and lonicera confusa dc, wherein the Keyaoluoqu identification method is the thin-layer chromatography method adopting a luteoloside reference substance as a control and adopting a mixed solution comprising ethyl acetate, acetone, formic acid and water according to a ratio of 7:3:1:1.2 as a developing solvent, the plantago depressa willd identification method is the thin-layer chromatography method adopting a plantamajoside reference substance as a control and adopting a mixed solution comprising ethyl acetate, acetone, formic acid and water according to a ratio of 7:3:1:1.2 as a developing solvent, the lonicera confusa dc identification method is the thin-layer chromatography method adopting an isochlorogenic acid A reference substance as a control and adopting a mixed solution comprising ethyl acetate, acetone, formic acid and water according to a ratio of 7:3:1:1.2 as a developing solvent; and the identification method of the present invention has characteristics of high sensitivity, good reproducibility, convenience, feasibility, and no negative control interference.

Description

Technical field

[0001] The invention relates to a method for identifying a Chinese medicine preparation for treating diabetes, and belongs to the technical field of Chinese medicine. Background technique

[0002] Diabetes is a common and frequently-occurring disease that seriously harms human health. At present, the number of diabetic patients in my country has increased year by year, and has exceeded 20 million. There are more than 100 million diabetic patients in the world. Diabetes has become a worldwide phenomenon. problem. Diabetes mortality is second only to malignant tumors and cardiovascular diseases, and is the third most life-threatening disease.

[0003] The patent application with the application number 200910312208.6 and titled "Quality Control Method of Chinese Medicine Preparations for the Treatment of Urinary Tract Infections" discloses a method for identifying plantain in Chinese medicine preparations for the treatment of urinary tract infections, but it does not d...

Examples

Embodiment 1

[0220] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add medicinal juice and water and stir to make a soft material, steam it thoroughly, keep the temperature at 22°C, humidity at 50%, add koji and ferment for 25 days, take out, dry, and crush to get it. The fresh Gualou can also be one or more of Gualou seed, Gualou peel and Trichosanthes powder.

[0221] The preparation method of the capsule for treating diabetes: weigh 480g of Keyaoluoqu, 350g of plantain, 335g of agrimony and 335g of honeysuckle, cut plantain and agrimony into 1.0cm segments, add 8 times the amount of water, Soak for 30 minutes, decoct for 2 hours, filter, and take the filtrate for later use; add 6 times the amount of water to the dregs and decoct for 1 hour, filter, combine the two filtrates, filter, and...

Embodiment 2

[0235] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add the medicinal juice and stir to make a soft material, steam it thoroughly, keep the temperature at 26°C and humidity at 65%, add koji and ferment for 30 days, take it out, dry, and crush to obtain Keyaoluoji.

[0236] The preparation method of the tablet for treating diabetes: weigh out 400g Coyaoluoqu, 300g Plantain, 400g Agrimony and 400g Honeysuckle, cut the Plantain and Agrimonia into 1.0cm sections, add 10 times the amount of water, Soak for 120 minutes, decoct for 2 hours, filter, and take the filtrate for later use; add 10 times the amount of water to the dregs and decoct for 2 hours, filter, combine the two filtrates, filter, and concentrate into a clear cream with a relative density of 1.3 at 60°C. Under the co...

Embodiment 3

[0244] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add medicinal juice and stir to make soft material, steam thoroughly, keep temperature at 18°C, humidity at 45%, add koji and ferment for 20 days, take out, dry, and crush to obtain Keyaoluoji.

[0245] The preparation method of granules for treating diabetes: weigh 500g of Keyaoluoqu, 400g of plantain, 300g of agrimony and 300g of honeysuckle, cut plantain and agrimony into 1.0cm segments, add 6 times the amount of water, Soak for 90 minutes, decoct for 1 hour, filter, and take the filtrate for later use; add 8 times the amount of water to decoct the medicinal residue for 1 hour, filter, combine the two filtrates, filter, and concentrate into a clear cream with a relative density of 1.2 at 60°C. Under the conditions of a t...

Technical field

[0001] The invention relates to a method for identifying a Chinese medicine preparation for treating diabetes, and belongs to the technical field of Chinese medicine. Background technique

[0002] Diabetes is a common and frequently-occurring disease that seriously harms human health. At present, the number of diabetic patients in my country has increased year by year, and has exceeded 20 million. There are more than 100 million diabetic patients in the world. Diabetes has become a worldwide phenomenon. problem. Diabetes mortality is second only to malignant tumors and cardiovascular diseases, and is the third most life-threatening disease.

[0003] The patent application with the application number 200910312208.6 and titled "Quality Control Method of Chinese Medicine Preparations for the Treatment of Urinary Tract Infections" discloses a method for identifying plantain in Chinese medicine preparations for the treatment of urinary tract infections, but it does not d...

Examples

Embodiment 1

[0220] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add medicinal juice and water and stir to make a soft material, steam it thoroughly, keep the temperature at 22°C, humidity at 50%, add koji and ferment for 25 days, take out, dry, and crush to get it. The fresh Gualou can also be one or more of Gualou seed, Gualou peel and Trichosanthes powder.

[0221] The preparation method of the capsule for treating diabetes: weigh 480g of Keyaoluoqu, 350g of plantain, 335g of agrimony and 335g of honeysuckle, cut plantain and agrimony into 1.0cm segments, add 8 times the amount of water, Soak for 30 minutes, decoct for 2 hours, filter, and take the filtrate for later use; add 6 times the amount of water to the dregs and decoct for 1 hour, filter, combine the two filtrates, filter, and...

Embodiment 2

[0235] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add the medicinal juice and stir to make a soft material, steam it thoroughly, keep the temperature at 26°C and humidity at 65%, add koji and ferment for 30 days, take it out, dry, and crush to obtain Keyaoluoji.

[0236] The preparation method of the tablet for treating diabetes: weigh out 400g Coyaoluoqu, 300g Plantain, 400g Agrimony and 400g Honeysuckle, cut the Plantain and Agrimonia into 1.0cm sections, add 10 times the amount of water, Soak for 120 minutes, decoct for 2 hours, filter, and take the filtrate for later use; add 10 times the amount of water to the dregs and decoct for 2 hours, filter, combine the two filtrates, filter, and concentrate into a clear cream with a relative density of 1.3 at 60°C. Under the co...

Embodiment 3

[0244] The preparation method of Keyaoluoqu: The mixture composed of 2g fresh melon sorrel, 2g fresh spatholobi and 1g fresh weeping grass is squeezed to extract juice, and the dried powder of medicinal residue is combined with 2g wheat flour and 1g bran. Mix, add medicinal juice and stir to make soft material, steam thoroughly, keep temperature at 18°C, humidity at 45%, add koji and ferment for 20 days, take out, dry, and crush to obtain Keyaoluoji.

[0245] The preparation method of granules for treating diabetes: weigh 500g of Keyaoluoqu, 400g of plantain, 300g of agrimony and 300g of honeysuckle, cut plantain and agrimony into 1.0cm segments, add 6 times the amount of water, Soak for 90 minutes, decoct for 1 hour, filter, and take the filtrate for later use; add 8 times the amount of water to decoct the medicinal residue for 1 hour, filter, combine the two filtrates, filter, and concentrate into a clear cream with a relative density of 1.2 at 60°C. Under the conditions of a t...

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