Method for preparing stevia rebaudiana polyphenols through composite chitosan flocculation method

A technology of chitosan and quaternary ammonium chitosan, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., to achieve the effects of low temperature, short flocculation time and high flocculation efficiency

Active Publication Date: 2019-03-08
DONGTAI HIRYE BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The above method of extracting polyphenols from Eucommia leaf water extract, Shuanghuanglian water extract, honeysuckle, Qinggan Lidan oral liquid raw medicine water extract only focuses on extracting polyphenols

Method used

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  • Method for preparing stevia rebaudiana polyphenols through composite chitosan flocculation method
  • Method for preparing stevia rebaudiana polyphenols through composite chitosan flocculation method
  • Method for preparing stevia rebaudiana polyphenols through composite chitosan flocculation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Stevia leaf extraction:

[0051] Stevia leaves were extracted according to the ratio of water to dry leaves of stevia leaves at 20BV, the extraction temperature was 50°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0052] (2) Composite chitosan flocculation

[0053] After the stevia leaf water extract is adjusted to pH4.5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 10% of composite chitosan quality, surplus is shell Polycan; the molecular weight of chitosan is 100KDa, and the degree of deacetylation is 80%), and the addition amount is 0.15mL per gram of dry leaves. At 20°C, stir at 30 rpm for 5 minutes, and let stand for 40 minutes to flocculate and remove impurities; then, separate the flocculation supernatant through plate and frame filtration; detect and calculate the retention rate of steviol glycosides...

Embodiment 2

[0071] (1) Stevia leaf extraction:

[0072] Stevia leaves were extracted according to the ratio of water to dry stevia leaves of 15BV, the extraction temperature was 60°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0073] (2) Composite chitosan flocculation:

[0074] After the stevia leaf water extract is adjusted to pH5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 15% of composite chitosan quality, surplus is chitosan ; the molecular weight of chitosan is 50KDa, the deacetylation degree is 70%), and the addition amount is 0.1mL per gram of dry leaves; at 40°C, stir at 30rpm for 5min, and let stand for 35min to flocculate and remove impurities; then, separate through plate and frame filtration The flocculation supernatant was obtained; the retention rate of steviol glycosides after flocculation was detected and calculat...

Embodiment 3

[0078] (1) Stevia leaf extraction:

[0079] The stevia leaves were extracted according to the ratio of water to dry stevia leaves of 20BV, the extraction temperature was 60°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0080] (2) Composite chitosan flocculation:

[0081] After the stevia leaf water extract is adjusted to pH4.5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 15% of composite chitosan quality, surplus is shell Polycan; Chitosan molecular weight is 80KDa, deacetylation degree is 80%), the addition amount is 0.12mL per gram of dry leaves; Stir at 30rpm for 5min at 40°C, let stand for 50min to flocculate and remove impurities; then, through the plate frame The flocculation supernatant was obtained by filtration and separation; the retention rate of steviol glycosides after flocculation was detected and calcul...

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Abstract

The invention discloses a method for preparing stevia rebaudiana polyphenols through a composite chitosan flocculation method and belongs to the technical field of plant extraction. The method comprises flocculating stevia rebaudiana water extract at room temperature with composite chitosan for impurity removal, and then absorbing supernatant through microporous resin to obtain effluent for further extraction of stevioside; then eluting the microporous resin with alcohol aqueous solutions of different concentrations, combining eluent eluted by low-concentration alcohol aqueous solutions with the undiluted effluent to obtain a combined solution with a content of total phenols lower than 0.5% on a dry basis for extracting stevioside; drying the eluent eluted by high-concentration alcohol aqueous solutions to obtain stevia rebaudiana polyphenols mainly containing isochlorogenic acid. The method for preparing the stevia rebaudiana polyphenols through the composite chitosan flocculation method achieves production of the stevia rebaudiana polyphenols with high content of isochlorogenic acid on the basis of an original stevioside production process, thereby achieving comprehensive utilization of stevia rebaudiana and increasing the economic benefits for enterprises.

Description

technical field [0001] The invention relates to a method for preparing stevia polyphenols by a composite chitosan flocculation method, belonging to the technical field of plant extraction. Background technique [0002] Stevia is a perennial herb that originated in South America and is widely planted in Central America, South Korea, Paraguay and other places. The content of inulin is 6%-12%. The steviol glycoside extracted from it is a low-calorie, high-sweet natural sweetener, which is widely used in food and pharmaceutical industries. Its sweetness is 150-300 times that of sucrose . In addition to the well-known steviol glycosides, alcohol, water or alcohol-water extracts of Stevia rebaudiana also contain polyphenols, flavonoids, quinic acid and caffeic acid and their derivatives, amino acids and fatty acids and their derivatives, and other monomers that need to be removed. Ning, tannins, pigments and pectin and other impurities. [0003] In stevia extract, steviol glyco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28
CPCA61K36/28A61K2236/331A61K2236/53A61K2236/55
Inventor 夏咏梅朱理平何东生陈科材周卓愉樊晔刘湘
Owner DONGTAI HIRYE BIOTECH CO LTD
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