Ultra-high performance liquid chromatography detecting method for traditional Chinese medicine composition
A detection method and composition technology, applied in the field of traditional Chinese medicine, can solve problems such as the inability to comprehensively and effectively reflect the quality consistency and effectiveness of traditional Chinese medicine compound preparations
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Embodiment 1
[0034] Prescription: 255g forsythia, 255g honeysuckle, 85g roasted ephedra, 85g fried bitter almond, 255g gypsum, 255g isatis root, 255g Mianma Guanzhong, 255g houttuynia cordata, 85g patchouli, 51g rhubarb, 85g rhodiola, mint Brain 7.5g, licorice 85g;
[0035] Preparation method: the above thirteen flavors, patchouli plus water distillation extract volatile oil, collect the volatile oil, filter the water extract, and set aside; forsythia, ephedra, houttuynia, and rhubarb are extracted twice with 70% ethanol, and the first time 2 hours, 1.5 hours for the second time, filter the extract, combine, recover ethanol, and set aside; add honeysuckle, gypsum, isatis root, Mianmaguanzhong, licorice, rhodiola, add water and boil until boiling, add fried bitter almonds, and boil Second time, 1.5 hours for the first time, 1 hour for the second time, filter the decoction, combine the filtrates, add the aqueous solution after patchouli oil extraction, concentrate to a relative concentration...
Embodiment 2
[0069] Example 2 Correlation analysis between chemical evaluation and biological evaluation of the quality of traditional Chinese medicine composition
[0070] The present invention uses PCR technology to measure IL-6 and COX-2 in mouse lung tissue, and finds that the composition capsule of the present invention can significantly reduce the expression of IL-6 and COX-2 in the lung tissue of viral pneumonia model mice , and present a dose-dependent relationship, so IL-6 and COX-2 are used as indicators for biological evaluation of the quality of the Chinese medicine composition capsules of the present invention.
[0071] Taking the contents of seven chemical components (chlorogenic acid, rutin, forsythiaside A, forsythiaside, isochlorogenic acid C, isochlorogenic acid B, and caffeic acid) in the traditional Chinese medicine composition as independent variables, biological The potency is the dependent variable, and 40 batches of traditional Chinese medicine compositions are used...
Embodiment 3
[0079] Recipe and preparation method are with embodiment 1.
[0080] Detection method: the chromatographic column is Waters Acquity HSS T3 column (2.1×100mm, 1.7μm), the mobile phase is a mixture of methanol (A) and 0.1% (v / v) phosphoric acid aqueous solution (B), and the gradient elution program is: 0.00 –8.00 minutes: 12~20%A; 8.01–15.00 minutes: 20~22%A; 15.01–20.00 minutes: 22~28%A; 20.01–25.00 minutes: 28~35%A; 25.01–30.00 minutes: 35~ 43%A; 30.01–35.00 minutes: 43~50%A; 35.01–40.00 minutes: 50~60%A; detection wavelength 210nm, injection volume 2.0μL, flow rate 0.20mL / min, column temperature 30℃, column equilibrium The time is 7 minutes.
[0081] Preparation of reference solution: Accurately weigh an appropriate amount of chlorogenic acid, caffeic acid, rutin, isochlorogenic acid B, isochlorogenic acid C, forsythiaside A and forsythin In a 25mL brown volumetric flask, add chromatographic grade methanol to make solutions containing 60μg, 75μg, 68μg, 125μg, 64μg, 80μg, an...
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