Method for establishing fingerprint of flos lonicerae medicinal preparation

A technology of pharmaceutical preparations and fingerprints, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to detect and control quality, difficult to apply to production practice, and time-consuming, so as to improve safety, stability, and stability High, high-precision effects

Active Publication Date: 2016-08-10
华润三九现代中药制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, on the one hand, the quality of honeysuckle formula granules cannot be detected and controlled as a whole through the conten

Method used

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  • Method for establishing fingerprint of flos lonicerae medicinal preparation
  • Method for establishing fingerprint of flos lonicerae medicinal preparation
  • Method for establishing fingerprint of flos lonicerae medicinal preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0055] In the following examples and experimental examples, the preparation method of honeysuckle formula granules is as follows: taking honeysuckle, heating and refluxing extraction for 2 times, adding 15 weight times of water for 0.5h for the first time, and adding 13 weight times of water for the second time to extract 0.5h, filter, combine the filtrate, concentrate the filtrate to 60°C with a relative density of 1.05-1.10, add conventional auxiliary materials, and make granules according to conventional technology.

Embodiment 2

[0057] The present embodiment establishes the method for the fingerprint of honeysuckle formula granules, comprising the following steps:

[0058] (1) Take 0.4 g of the medicinal preparation of honeysuckle to be tested, accurately weigh it, accurately add 25 mL of 50% methanol aqueous solution, weigh it, heat under reflux for extraction or ultrasonic extraction for 30 minutes, let it cool, weigh it again, and take 50% methanol The water solution supplements the lost weight, shakes well, filters, and takes the subsequent filtrate as the test solution;

[0059] (2) Precisely weigh the chlorogenic acid reference substance, add 50% methanol aqueous solution to make a solution containing 0.00004 g per 1 mL, shake well, and use it as the reference substance A solution;

[0060] Precisely weigh the reference substance of luteolin, add 50% methanol aqueous solution to make a solution containing 0.00004g per 1mL, shake well, and use it as the reference substance B solution;

[0061] P...

Embodiment 3

[0066] The present embodiment establishes the method for the fingerprint of honeysuckle formula granules, comprising the following steps:

[0067] (1) Take 0.3 g of the medicinal preparation of honeysuckle to be tested, accurately weigh it, accurately add 20 mL of 60% methanol aqueous solution, weigh it, heat under reflux for extraction or ultrasonic extraction for 40 minutes, let it cool, weigh it again, and take 60% methanol The water solution supplements the lost weight, shakes well, filters, and takes the subsequent filtrate as the test solution;

[0068] (2) Precisely weigh the chlorogenic acid reference substance, add 60% methanol aqueous solution to make a solution containing 0.00002 g per 1 mL, shake well, and use it as the reference substance A solution;

[0069] Precisely weigh luteolin reference substance, add 60% methanol aqueous solution to make a solution containing 0.00002g per 1mL, shake well, and use it as reference substance B solution;

[0070] Accurately w...

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Abstract

The invention relates to a method for establishing a fingerprint of a flos lonicerae medicinal preparation. The method comprises the following steps of by taking flos lonicerae formula granules as detection objects, establishing a method for a fingerprint of a medicinal preparation, and obtaining more comprehensive fingerprint information; determining common characteristic peaks: first peak neochlorogenic acid, second peak chlorogenic acid, third peak cryptochlorogenic acid, fourth peak rutin, fifth peak galuteolin, sixth peak, seventh peak isochlorogenic acid A and eighth peak isochlorogenic acid C; selecting the second peak chlorogenic acid as an inner reference peak, and determining relative retention time of the common characteristic peaks of the flos lonicerae formula granules. In addition, by combining information of multiple chromatographic peaks in the fingerprint, the quality of the flos lonicerae formula granules can be detected comprehensively and quickly, and comprehensive quality detection and integral quality control of the flos lonicerae formula granules are facilitated, thereby benefiting the improvement on the safety and the stability of medicine use. Meanwhile, the method has the advantages of high stability, high precision, good repeatability and the like.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for establishing the fingerprint of a medicinal preparation of honeysuckle. Background technique [0002] Honeysuckle formula granules are obtained by extracting, concentrating and granulating traditional Chinese medicine honeysuckle. Honeysuckle is the dried flower buds or flowers with early blooming of Lonicera japonica Thunb., which mainly contains volatile oils, flavonoids, three mushrooms, organic acids, cycloalkenyl mushrooms, alcohols and trace elements. It has the functions of clearing away heat and detoxifying, dispersing wind-heat, cooling blood to stop dysentery, lowering blood and reducing fire, and eliminating sore throat and phlegm. [0003] The quality control of honeysuckle in the 2015 edition of the Chinese Pharmacopoeia includes the original plant varieties, medicinal properties, physical and chemical identification, content determination and other items. ...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 谭沛郑晓英张辉马鹏岗高雅石子仪
Owner 华润三九现代中药制药有限公司
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