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Method for purifying forsythiaside A from forsythia extractive

A technology of forsythia extract and forsythiaside, which is applied in the field of medicine, can solve the problems of limiting the extraction process of forsythiaside A and reducing extraction efficiency, and achieve the effect of saving raw materials and improving extraction efficiency

Active Publication Date: 2007-12-05
SHANDONG NEWTIME PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to avoid the hydrolysis of forsythiaside A in the prior art, the temperature must be controlled to avoid contact with strong acid and strong alkali, which to a large extent limits the extraction process of forsythiaside A and reduces the extraction efficiency

Method used

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  • Method for purifying forsythiaside A from forsythia extractive
  • Method for purifying forsythiaside A from forsythia extractive

Examples

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Effect test

Embodiment 1

[0016] The crude silica gel was dry-packed into a glass column (aspect ratio of 1:15), and connected to a UV chromatograph. Forsythia extract dry powder 10 grams, mixed with 20 grams of 100 mesh coarse silica gel in a mortar, solid loading, sample volume 1:30, with ethyl acetate-acetone-water (8:2:0.5) as the flow Phase elution, control flow rate 10ml / min, collect forsythiaside A peak, combine, concentrate under reduced pressure, vacuum dry; then apply RP-18 silica gel column (diameter-height ratio 1:12, loading amount 1:50) for purification , eluted with methanol-water (3:7), collected the peaks of forsythiaside A, combined, concentrated under reduced pressure, and dried in vacuum to obtain forsythiaside A product.

Embodiment 2

[0018] The crude silica gel was dry-packed into a glass column (aspect ratio of 1:15), and connected to a UV chromatograph. Forsythia extract dry powder 10 grams, mixed with 20 grams of 100 mesh coarse silica gel in a mortar, solid loading, sample volume 1:30, with ethyl acetate-acetone-water (9:0.5:0.5) as the flow Phase elution, control flow rate 8ml / min, collect forsythiaside A peak, combine, concentrate under reduced pressure, vacuum dry; Then apply RP-18 silica gel column (diameter-to-height ratio 1: 12, loading amount is 500 grams) to purify , eluted with methanol-water, the ratio is 4:6, the peaks of forsythiaside A are collected, combined, concentrated under reduced pressure, and dried in vacuum to obtain forsythiaside A.

Embodiment 3

[0020] The crude silica gel was dry-packed into a glass column (aspect ratio of 1:15), and connected to a UV chromatograph. Forsythia extract dry powder 10 grams, mixed with 200 mesh coarse silica gel 50 grams in a mortar, solid loading, sample volume 1:60, with ethyl acetate-methanol-water (1:4.5:4.5) as the flow Phase elution, control flow rate 12ml / min, collect forsythiaside A peak, combine, concentrate under reduced pressure, vacuum dry; then put on RP-18 silica gel column (diameter-to-height ratio 1:12, loading volume: 1:100) Purify, elute with methanol-water, the ratio is 1:9, collect the peaks of forsythiaside A, combine them, concentrate under reduced pressure, and dry in vacuum to obtain forsythiaside A.

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Abstract

The present invention relates to process of purifying forsythia extract to obtain forsythoside A. Specifically, forsythia phenols are further purified to obtain high purity forsythoside A in high extracting efficiency.

Description

technical field [0001] The invention relates to a purification method of a traditional Chinese medicine forsythia extract, which belongs to the technical field of medicine. Background technique [0002] Forsythia (Fructus Forsythiae) is a plant of the genus Forsythia in the family Oleaceae. In traditional Chinese prescriptions, the fruit of forsythia is often used as medicine, and it is used together with other heat-clearing and detoxifying drugs to clear away heat and detoxify, dissipate stagnation and reduce swelling. It is a commonly used traditional Chinese medicine recorded in the 2000 edition of "Chinese Pharmacopoeia". Scholars at home and abroad have done a lot of research on the chemical constituents and pharmacology of Forsythia, and have realized that Forsythia has very significant antibacterial and antiviral activities. Its main antibacterial and antiviral active ingredient is forsythiaside, and almost all of forsythiaside is forsythiaside A, which has a strong ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 玄振玉王勇黄孝春于莉林君
Owner SHANDONG NEWTIME PHARMA
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