Extraction method of lamiophlomis rotata phenolic glycoside and application in medicine or health care product for preventing and treating hepatic fibrosis

A technology for liver fibrosis and health care products, which is applied in the field of extraction of unique total phenolic glycosides extract and phenolic glycosides mixture of unique total phenolic glycosides extract

Active Publication Date: 2022-01-21
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] From 2010 to 2013, Zhang Yi's research group found that Duyiwei granule has a good anti-hepatic fibrosis effect (Yang Han, Zhang Yi, Tan Wanchu, et al. Experimental research on Duyiwei granule's anti-hepatic fibrosis in rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2010, 044:64-67; Zhang Yi, Dai Xiahuan, Xie Xiucui, et al. Effects of Duyiwei Granules on TGF-β1 / Smads signaling pathway in rats with experimental liver fibrosis[J]. Chinese Traditional Medicine Journal of Basic Medicine, 2013,019(012):1401-1403,1407.), but no related follow-up reports
So far, 127 chemical constituents including iridoids, flavonoids and phenylethanol glycosides have been identified from Lamiophlomis rotata (Zhan-HuCui, et al. Traditional uses, phytochemistry, pharmacology and toxicology of Lamiophlomis rotata (Benth.) Kudo: a review. RSC Advances.2020,10:11463–11474), which type or components have the effect of anti-hepatic fibrosis, so far, there is no report

Method used

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  • Extraction method of lamiophlomis rotata phenolic glycoside and application in medicine or health care product for preventing and treating hepatic fibrosis
  • Extraction method of lamiophlomis rotata phenolic glycoside and application in medicine or health care product for preventing and treating hepatic fibrosis
  • Extraction method of lamiophlomis rotata phenolic glycoside and application in medicine or health care product for preventing and treating hepatic fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Extraction preparation and UPLC-TOF / MS analysis of the unique total phenolic glycoside extract of the present invention

[0031] 1. Extraction and preparation of Duyiwei total phenolic glycosides extract

[0032] Method 1 Weigh 5 kg of the aboveground part of Duyiwei, crush it, add water 10 times the volume of the medicinal material, decoct twice for 2 hours each time, filter and combine the filtrate, let it stand for 12 hours, take the supernatant and pass it through the treated polyamide (10 -30 mesh) adsorption, first eluted with 5 times the amount of water of the adsorption resin bed, then eluted with 75% ethanol for analysis, collected the analysis solution, recovered the solvent, concentrated at 90°C, and dried to obtain the total phenol Glycoside extract 203.6g.

[0033] Method 2 Weigh 5kg of the aboveground part of Duyiwei, crush it, add water 10 times the volume of the medicinal material, decoct twice for 2 hours each time, filter and combine the fi...

Embodiment 2

[0044] Example 2: Effects of the total phenolic glycoside extract and phenolic glycoside composition of Duyiwei on the proliferation and apoptosis of hepatic stellate cells

[0045] 1. Solution configuration

[0046] (1) Preparation of the total phenolic glycosides extract solution of Duyiwei

[0047] Weigh 20.0mg of the dried total phenolic glycosides extract prepared by method 2 in Example 1 and place it in a 10mL volumetric flask, add 10mL of DMSO to the mark and shake well to obtain the preparation solution of the total phenolic glycosides extract of unique herbs. 2.0 mg / mL.

[0048] Dilute the preparation solution of the total phenolic glycosides extract of the unique herb to different concentrations of the total phenolic glycosides extract of the unique one: 10 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL.

[0049] (2) Preparation of phenolic glycoside composition solution

[0050] Experimental drugs: Actascoside, Forsythiaside B, Icariin H1 and Decaffeoylverbascos...

Embodiment 3

[0065] Embodiment three: the unique total phenolic glycosides extract to CCl 4 Effects on Liver Fibrosis Models

[0066] C57 male mice (body weight 22-25g) were randomly divided into control group, CCl4 Induction group, high, medium and low (50, 100, 200 mg / kg) dose groups of total phenolic glycosides extract of Duyiwei, 10 rats in each group. In addition to the normal control group, CCl 4 Prepared with corn oil to a concentration of 10%, CCl 4 Corn oil solution was injected intraperitoneally at 0.5 mL / kg, and the models were established every Monday and Thursday for 6 weeks. In the first CCl 4 Accompanied by CCl after injection 4 Administer after injection.

[0067] CCl 4 Two days after induction, liver tissues were harvested for HE staining of pathological sections. The results showed that the hepatic lobule structure in the normal group was clear, the hepatic plates were arranged neatly in a cord shape, the portal area did not expand, and there was no inflammatory ce...

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Abstract

The invention belongs to the field of medicines and health-care products, and particularly relates to application of a phenolic glycoside mixture in preparation of medicines or health-care products for preventing and treating hepatic fibrosis, in particular to a phenolic glycoside mixture of a total phenolic glycoside extract of lamiophlomis rotata, and an extraction method of the total phenolic glycoside extract of lamiophlomis rotata. The total phenolic glycoside extract of the lamiophlomis rotata comprises eight symbolic components, namely verbascoside, forsythiaside B, 4-hydroxybenzoic acid, icariin H1, Decaffeoylverbascoside, cosmosiin, luteolin and apigenin, has an anti-hepatic fibrosis effect, and can be used for preparing a medicine for preventing and treating hepatic fibrosis or a health-care product for assisting in treating CCl4 liver injury. According to the extraction method of the lamiophlomis rotata total phenolic glycoside extract, the polyacrylamide gel resin is adopted, so that separation and purification of the lamiophlomis rotata total phenolic glycoside extract are smoother, and the yield is higher.

Description

technical field [0001] The present invention relates to the field of traditional Chinese medicine and health care products, in particular to the application of phenolic glycoside mixture in the preparation of medicines or health care products for preventing and treating liver fibrosis, especially the phenolic glycoside mixture of unique total phenolic glycoside extract, and also relates to a unique unique total phenolic glycoside extract Extraction method of glycoside extract. Background technique [0002] Long-term hepatitis B virus invasion, non-alcoholic fat, or hepatic cholestasis damages the liver, causing liver tissue repair ability disorder, resulting in fibronectin (FN), α-smooth muscle actin (α-SMA) and collagen-α1 (Coll-α1) and other extracellular matrix are excessively deposited, eventually forming liver fibrosis. Studies have shown that the overactivation of transforming growth factor-β (transforming growth factor-β, TGF-β) is one of the main factors in the deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P1/16A23L33/105
CPCA61K36/53A61P1/16A23L33/105A61K2236/331A61K2236/39A61K2236/55A61K2236/51A23V2002/00A23V2200/30A23V2250/21A23V2300/14A23V2300/50
Inventor 潘正邓杰谢亚均万果果陈志伟曹文富
Owner CHONGQING MEDICAL UNIVERSITY
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