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Application of forsythia flower yellow pigment in preparing anti-oxidative stress injury drug

An anti-oxidative stress and yellow pigment technology, applied in the field of effective parts of natural medicinal plants, can solve problems such as unseen

Pending Publication Date: 2019-04-12
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some reports on the extraction and stability of forsythia anthocyanin, but there is no report on its anti-oxidative stress activity in the model organism nematode (Caenorhabditis elegans, C. life expectancy report

Method used

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  • Application of forsythia flower yellow pigment in preparing anti-oxidative stress injury drug
  • Application of forsythia flower yellow pigment in preparing anti-oxidative stress injury drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Forsythia yellow pigment preparation:

[0016] Take 20g of dried forsythia flower and grind it into powder, add 2000mL of 95% ethanol, heat the water bath at 50°C, extract by heat reflux; separate by suction filtration, repeat the extraction twice, collect the extract, concentrate it by rotary evaporation, freeze-dry, and collect the dry powder That is the yellow pigment; identified by high pressure liquid chromatography forsythia yellow pigment components mainly include: rutin (236.09mg / g, mass fraction is 23.61%), forsythiaside A (116.95mg / g, mass fraction is 11.69%), pinoresin (82.51mg / g, mass fraction is 8.25%), forsythin (18.23mg / g, mass fraction is 1.83%).

Embodiment 2

[0018] Effects of forsythia anthocyanin on ROS in nematodes

[0019] Nematodes that have grown synchronously to the L4 stage are transferred to NGM medium that does not contain or contain the yellow pigment, cultured in a constant temperature incubator at 20°C for 2 days, washed with M9 buffer to collect the nematodes, and performed on ice with a homogenizer Grind and centrifuge at 12 000r / m to take the supernatant. Take a part to measure protein concentration with BCA kit. A certain amount of the above supernatant was added to a black 96-well plate, and diluted with M9 buffer to make the protein concentration in each well consistent, and DCFH-DA fluorescent probe was added to make the final concentration 25 μM. The volume of each well was 200 μL, and three replicate wells were set up for each group. Incubate at 20°C for 30min. The multifunctional microplate reader detects the fluorescence intensity at an excitation wavelength of 485nm and an emission wavelength of 528nm. ...

Embodiment 3

[0024] Effects of forsythia anthocyanin on the resistance to oxidative stress in wild-type nematodes

[0025] NGM medium containing 500 μM juglone was prepared, and the wild-type N2 nematodes synchronized to the L4 phase were picked to plates without and with the yellow pigment, 30 per plate. The number of surviving nematodes was counted every hour, and the survival rate was calculated until all nematodes died.

[0026] Experimental results such as figure 2 As shown, the average lifespan of nematodes in the control group was 2.05h; the average lifespan of nematodes after the action of 20μg / mL yellow pigment was 3.25h, which was statistically different from the control (P<0.05); the effect of 40μg / mL yellow pigment on nematodes The average lifespan after treatment was extended to 7.25h, compared with the control group, there was a very significant difference (P<0.01); the average lifespan was 4.65h after 80 μg / mL yellow pigment acted on the nematodes, and there was a very sig...

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Abstract

The invention provides an application of forsythia flower yellow pigment in preparing an anti-oxidative stress injury drug. The preparation step of the yellow pigment is as follows: adding dried forsythia flower powder into 95% ethanol according to a solid-liquid ratio of 1:100, performing water bath at 50 DEG C, and performing extraction by a hot reflux method; and performing suction filtration separation, repeating extraction twice, collecting the extractive liquid, performing rotary evaporation concentration, carrying out freeze drying, and collecting dry powder. The yellow pigment contains: rutin, forsythoside A, rosin, forsythin and the like. The yellow pigment can effectively eliminate ROS free radicals in caenorhabditis elegans body by up-regulating the expression of DAF-16 and SKN-1 target genes, increase the activity of antioxidant enzymes in elegans body, reduce the free radical content in the elegans body and weaken the oxidative stress damage in the elegans body by juglone,and can significantly prolong the lifespan of wild-type elegans N2 and daf-16 mutant elegans under oxidative stress, thereby increasing the resistance of elegans to oxidative stress. The yellow pigment can also be used in the preparation of functional foods resistant to oxidative stress damage.

Description

technical field [0001] The present invention relates to the application of effective parts of natural medicinal plants, in particular to the application of a yellow pigment derived from forsythia flower in the preparation of anti-oxidative stress damage medicine or functional food. Background technique [0002] Oxidative stress is considered to be an important factor leading to body aging, tissue damage and disease. The occurrence of oxidative stress is due to the excessive free radicals produced by the body after endogenous or exogenous damage, releasing a large number of reactive oxygen species (ROS) and other oxidative substances, and the body's own ability to resist oxidation is reduced. If the excessive accumulation of ROS cannot be removed, the oxidation reaction of the body far exceeds the anti-oxidation scavenging ability, resulting in an imbalance between oxidation and anti-oxidation ability. The accumulation of ROS caused by oxidative stress can cause vascular end...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/634A61P39/06A23L33/105
CPCA23V2002/00A61K36/634A23L33/105A61P39/06A23V2200/30A23V2250/21
Inventor 李玉英张立伟张东丹
Owner SHANXI UNIV
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