Establishhment method for fructus forsythiae medicine fingerprint

Abstract

The invention belongs to the technical field of medicine analysis and specifically relates to an establishment method for a fructus forsythiae medicine fingerprint. The method specifically is realizedby the following steps of preparing a reference substance solution, taking appropriate amount of forsythin, forsythoside A, isoforsythiaside, rutin and forsythingenin reference substances, and preparing the reference substances into the reference substance solution; preparing a test sample solution, weighing fructus forsythiae medicine powder, carrying out heating reflux extraction, taking subsequent filtrate, carrying out filtering through utilization of filter membrane, taking the subsequent filtrate as a sample solution, measuring the sample solution and a puerarin water solution, and carrying out mixing uniformly to obtain the test sample solution; and carrying out detection through adoption of a high performance liquid chromatography and establishing the fingerprint. According to themethod, high performance liquid chromatography detection is carried out on the fructus forsythiae medicine through adoption of different gradient elution, a separation effect is good; through external addition of an internal standard, the established fingerprint has a semiquantitative function, so change of components among samples can be effectively expressed, and quality of the samples can be judged relatively precisely; and quality monitoring can be carried out on the raw material fructus forsythiae medicine relatively comprehensively.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a method for establishing fingerprints of forsythia medicinal materials. Background technique [0002] Forsythia is the dried fruit of Forsythia suspensa (Thunb.) Vahl, which is a plant of Oleaceae. It is harvested in autumn when the fruit is first ripe and green, removed impurities, steamed, and dried. It is commonly called "Qingqiao"; the fruit is ripe Harvest in season, dry in the sun, remove impurities, commonly known as "Laoqiao" [1] . Forsythia has the effects of clearing heat and detoxifying, reducing swelling and dispelling stagnation, and evacuating wind-heat. [2] . Modern pharmacological research shows that forsythia has antibacterial, antiviral, anti-inflammatory, antipyretic, antiemetic, diuretic and other effects [3] . At present, Forsythia has definite curative effect, low toxicity and side effects, and wide range of uses and clinical applicat...

AI Technical Summary

Problems solved by technology

[0003] At present, in the quality control project of forsythia medicinal materials, the 2015 edition of "Chinese Pharmacopoeia" only uses the determination of forsythin and forsythiaside A content as the quality control of forsythia medicinal materials. The intrinsic quality of the medicinal materials of Rhizoma chinensis has been researched on its fingerprints, and each has its own characteristics.

The literature "Research on HPLC Fingerprint Spectrum of Forsythia" provides a method for establishing fingerprints. This method uses the ingredients contained in the drug itself as the internal standard. This method will cause differences in the reference value due to the differences between batches of samples. Larger changes, the obtained relative peak area can only represent the proportion change of each component in a single sample, but cannot represent the change in the amount of components between samples, so this method can only perform qualitative comparisons, and cannot accurately judge the quality of medicinal materials

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