Method for purifying forsythiaside A from forsythia extractive

A technology of forsythia extract and forsythiaside, applied in the field of medicine, can solve the problems of reducing the extraction efficiency, limiting the extraction process of forsythiaside A, etc., and achieves the effect of improving efficiency and saving and utilizing raw materials

Active Publication Date: 2007-12-05
SHANDONG NEWTIME PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to avoid the hydrolysis of forsythiaside A in the prior art, the temperature must be controlled to avoid contact with strong acid and strong alkali, which to a large extent limits the extraction process of forsythiaside A and reduces the extraction efficiency

Method used

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  • Method for purifying forsythiaside A from forsythia extractive
  • Method for purifying forsythiaside A from forsythia extractive

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Get the Forsythia extract, mix the sample and put it on a 100-mesh silica gel column, use ethyl acetate-acetone-water (8:2:0.5) as the mobile phase to elute, collect 100ml of each fraction, combine 13-21 fractions, Concentrate to dryness under reduced pressure, and the residue is subjected to thin-layer silica gel column chromatography, eluting with ethyl acetate-acetone-water (8:2:0.5) as the mobile phase, collecting 10ml of each fraction, and combining 5-15 fractions, Concentrated to dryness under reduced pressure, the residue was purified by RP-18 silica gel column, eluted with methanol-water (3:7), 10ml was collected for each fraction, 47-49 fractions were combined, concentrated to dryness under reduced pressure, and dried in vacuo, that is Forsythiaside A was obtained.

Embodiment 2

[0018] Get the Forsythia extract, put it on a 200-mesh silica gel column after mixing the sample, and use ethyl acetate-methanol-water (7:2:1) as the mobile phase to elute, collect 100ml of each fraction, combine 12-21 fractions, Concentrate under reduced pressure to dryness, and the residue is subjected to thin-layer silica gel column chromatography, eluting with ethyl acetate-methanol-water (7:2:1) as the mobile phase, collecting 10 ml of each fraction, and combining 6-15 fractions, Concentrated to dryness under reduced pressure, the residue was purified by RP-18 silica gel column, eluted with methanol-water (2:8), 10ml of each fraction was collected, 47-49 fractions were combined, concentrated to dryness under reduced pressure, and dried in vacuo, that is Forsythiaside A was obtained.

Embodiment 3

[0020] Take the Forsythia extract, mix the sample and put it on a 200-mesh silica gel column, elute with chloroform-methanol-water (5:2.5:2.5) as the mobile phase, collect 100ml of each fraction, combine 12-21 fractions, and depressurize Concentrated to dryness, the residue was subjected to thin-layer silica gel column chromatography, eluted with chloroform-methanol-water (5:2.5:2.5) as the mobile phase, and 10ml of each fraction was collected, 6-15 fractions were combined, and concentrated under reduced pressure to Dry, the residue is purified by RP-18 silica gel column, eluting with methanol-water (2:8), collecting 10ml of each fraction, combining 47-49 fractions, concentrating under reduced pressure to dryness, and drying in vacuo to obtain Forsythia Glycoside A.

[0021] Identification of forsythiaside A

[0022] Accurately weigh an appropriate amount of forsythiaside A prepared according to the method in the example, add methanol to make a solution containing 0.62 mg of ...

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Abstract

The present invention relates to process of purifying forsythia extract to obtain forsythoside A. Specifically, forsythia phenols are further purified to obtain high purity forsythoside A in high extracting efficiency.

Description

technical field [0001] The invention relates to a purification method of a traditional Chinese medicine forsythia extract, which belongs to the technical field of medicine. Background technique: [0002] Forsythia (Fructus Forsythiae) is a plant of the genus Forsythia in the family Oleaceae. In traditional Chinese prescriptions, the fruit of forsythia is often used as medicine, and it is used together with other heat-clearing and detoxifying drugs to clear away heat and detoxify, dissipate stagnation and reduce swelling. It is a commonly used traditional Chinese medicine recorded in the 2000 edition of "Chinese Pharmacopoeia". Scholars at home and abroad have done a lot of research on the chemical constituents and pharmacology of Forsythia, and have realized that Forsythia has very significant antibacterial and antiviral activities. Its main antibacterial and antiviral active ingredient is forsythiaside, and almost all of forsythiaside is forsythiaside A, which has a strong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 玄振玉王勇黄孝春于莉林君
Owner SHANDONG NEWTIME PHARMA
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