A method for extracting polyphenols from walnut kernels with testa by combining enzymes and ultrasonic treatment

A technology of ultrasonic treatment and walnut kernel, which is applied in food science, fat oil/fat production, fat production, etc. It can solve the problems of low content, immature extraction of walnut polyphenols, etc., and achieve economic value, extraction effect and efficiency improvement , The effect of improving the convenience of application

Active Publication Date: 2020-08-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As pointed out by Gao Haiyan et al. (Gao Haiyan, Li Xingfei, Chen Hangjun, Fang Xiangjun. Progress in the Extraction and Antioxidation of Pecan Polyphenols. Food Science, 2011,32(05):336-341), microwave extraction technology and super The critical extraction technology has reached the applicable level in the extraction of walnut oil, but due to the limitation of unfavorable factors such as the relatively low content of polyphenols and the local temperature rise caused by microwave treatment, these technologies are applied to the extraction of walnut polyphenols immature

Method used

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  • A method for extracting polyphenols from walnut kernels with testa by combining enzymes and ultrasonic treatment
  • A method for extracting polyphenols from walnut kernels with testa by combining enzymes and ultrasonic treatment

Examples

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Effect test

Embodiment 1

[0054] Example 1: Extraction of the crude walnut polyphenol extract of the present invention

[0055] Weigh 1000 g of commercially available walnut kernels with inner seed coat (about 5% broken kernel rate), according to the ratio of the mass of the walnut kernel in g to the volume of the ethanol aqueous solution in mL ratio 1:4, compare it with the volume of 4L. Mix the ethanol solution with a concentration of 5%, adjust the pH of the system to 4.0 with 1M hydrochloric acid, raise the temperature of the system to 55°C, and add Novozymes' pectinase at a ratio of 0.2U / g walnut kernels. SMASH, place the obtained polyphenol extraction system in an ultrasonic cleaner (such as Kunshan Shumei KQ-J2000GKDE high-power constant temperature ultrasonic cleaner), adjust the ultrasonic power to 250W according to the ratio of ultrasonic power 0.25W / g walnut kernel, and ultrasonically treat it for 120min , and then gauze filtration to recover the crude extract of dephenolized walnut kernels a...

Embodiment 2

[0056] Example 2: Extraction of the crude walnut polyphenol extract of the present invention

[0057] The commercially available Xinjiang paper-skinned walnuts are peeled to obtain 200g of walnut kernels (about 2% of the broken kernel rate), according to the ratio of the mass of the walnut kernels in g to the volume ratio of the ethanol aqueous solution in mL ratio 1:15, it is combined with the ratio of 1:15. Mix 3L of ethanol solution with a volume concentration of 20%, adjust the pH of the system to 6.0 with 1M citric acid, raise the temperature of the system to 40°C, and add Novozymes' cellulase Cellic CTec2 at a ratio of 5U / g walnut kernels. , place the mixed system in an ultrasonic cleaning machine, adjust the ultrasonic power to 2000W according to the ratio of ultrasonic power 10W / g walnut kernels, ultrasonically treat for 20min, and then filter with a Buchner funnel to recover the walnut kernels and the extract respectively. The leaching solution was vacuum rotary evapo...

Embodiment 3

[0058] Example 3: Extraction of the crude walnut polyphenol extract of the present invention

[0059] According to the ratio of the mass of walnut kernels in g to the volume of ethanol aqueous solution in mL ratio of 1:10, 500 g of commercially available walnut kernels with inner seed coat (the rate of broken kernels are about 10%) and 5L of 10% Mix the ethanol solution, adjust the pH of the system to 4.5 with 1M citric acid, raise the temperature of the system to 50°C, and add the compound enzyme of Novozymes at the ratio of 0.1U / g and 0.2U / g walnut kernels respectively. SMASH and Max, put the mixed system in an ultrasonic cleaning machine, adjust the ultrasonic power to 1000W according to the ratio of ultrasonic power 2W / g walnut kernels, ultrasonically treat for 30min, and then filter the walnut kernels and extract with gauze respectively. The walnut kernels were extracted twice under the same conditions as above, and the dephenolized walnut kernels and the extract were ...

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Abstract

A method for extracting polyphenol substances from semen juglandis with inner seed coats by the aid of combined enzyme and ultrasonic treatment. The method comprises: obtaining semen juglandis with seed coats; feeding the semen juglandis with inner seed coats into an ethanol aqueous solution according to a volume ratio of 1:3-1:15, regulating the pH of the mixture by the aid of food-grade citric acid or hydrochloric acid until the pH of the mixture is 3.5-6.5, and then adding pectinase and / or a cellulase compound to obtain a polyphenol extracted system. According to the semen juglandis polyphenol crude extract prepared by the method, the total weight of dry substances, which are obtained after freeze drying, at least accounts for 2% of the weight of the original semen juglandis with the coats, and the contents of polyphenol effective substances in the semen juglandis polyphenol crude extract can reach 15% at least; the semen juglandis polyphenol crude extract further can be separated by the aid of macroporous adsorption resin and industrial chromatographs, and accordingly products such as ellagic acid, hydrolysable tannin, and juglone can be prepared.

Description

【Technical field】 [0001] The invention belongs to the technical field of oil crop processing. More specifically, the present invention relates to a method for extracting polyphenols from walnut kernels with inner seed coat by combining enzymes and ultrasonic treatment, and also relates to the application of the crude walnut polyphenol extract obtained by the method. 【Background technique】 [0002] Walnut (Juglans regia L.), also known as walnut and walnut, is a Juglans plant belonging to the genus Juglans, ranking first among the "four major dried fruits". It is an excellent economic crop with both medicine and food. Walnut kernels are rich in nutrients such as fat, protein and vitamins, and have various effects such as strengthening the stomach, nourishing blood, moistening the lungs, nourishing the kidneys, and nourishing the brain. They are a good tonic, rich in nutrients and delicious. "Long Live", "Longevity Fruit", "Puzzle Fruit" and "Beauty Fruit". Modern research s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L25/00A23L33/105A23L5/30C11B1/10
CPCA23V2002/00C11B1/10A23V2250/2132
Inventor 张文斌蒋将李笑笑杨瑞金华霄赵伟
Owner JIANGNAN UNIV
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