Method for extracting polyphenol substances from semen juglandis with inner seed coats by aid of combined enzyme and ultrasonic treatment

A technology of ultrasonic treatment and walnut kernel, which is applied in food science, fat production, fat oil/fat production, etc., can solve the problems of immature extraction and low content of walnut polyphenols, achieve extraction effect and efficiency improvement, and enhance economic value , The effect of improving the convenience of application

Active Publication Date: 2017-06-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As pointed out by Gao Haiyan et al. (Gao Haiyan, Li Xingfei, Chen Hangjun, Fang Xiangjun. Progress in the Extraction and Antioxidation of Pecan Polyphenols. Food Science, 2011,32(05):336-341), microwave extraction technology and super The critical extraction technology has reached the applicable level in the extraction of walnut oil, but due to the limitation of unfavorable factors such as the relatively low content of polyphenols and the local temperature rise caused by microwave treatment, these technologies are applied to the extraction of walnut polyphenols immature

Method used

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  • Method for extracting polyphenol substances from semen juglandis with inner seed coats by aid of combined enzyme and ultrasonic treatment
  • Method for extracting polyphenol substances from semen juglandis with inner seed coats by aid of combined enzyme and ultrasonic treatment

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Effect test

Embodiment 1

[0059] Embodiment 1: the extraction of walnut polyphenol crude extract of the present invention

[0060]Weigh 1000 g of commercially available walnut kernels with testa (about 5% crushed kernel rate), and mix it with 4L volume Mix the ethanol solution with a concentration of 5%, adjust the pH of the system to 4.0 with 1M hydrochloric acid, raise the temperature of the system to 55°C, and add pectinase from Novozymes at a rate of 0.2U / g walnut kernel SMASH, put the obtained polyphenol extraction system in an ultrasonic cleaner (such as Kunshan Shumei KQ-J2000GKDE high-power constant temperature ultrasonic cleaner), adjust the ultrasonic power to 250W according to the ratio of ultrasonic power to 0.25W / g walnut kernel, and ultrasonically treat for 120min , and then filtered through gauze to recover the crude extracts of dephenolized walnut kernels and walnut polyphenols, respectively. The obtained dephenolized walnut kernels are leached again under the same conditions as above...

Embodiment 2

[0061] Embodiment 2: the extraction of walnut polyphenol crude extract of the present invention

[0062] Commercially available Xinjiang paper-skinned walnuts are peeled off to obtain 200 g of walnut kernels (about 2% broken kernel rate), according to the ratio of 1:15 between the quality of walnut kernels in g and the volume ratio of ethanol aqueous solution in mL, it is mixed with Mix 3L of ethanol solution with a volume concentration of 20%, adjust the pH of the system to 6.0 with 1M citric acid, raise the temperature of the system to 40°C, and add Cellulase CTec2 from Novozymes at a rate of 5U / g walnut kernels , place the mixed system in an ultrasonic cleaning machine, adjust the ultrasonic power to 2000W according to the ratio of ultrasonic power to 10W / g walnut kernels, ultrasonically treat for 20min, and then filter with a Buchner funnel to recover walnut kernels and extracts respectively. The ethanol was removed by rotary evaporation in vacuum, the extract was concentr...

Embodiment 3

[0063] Embodiment 3: the extraction of walnut polyphenol crude extract of the present invention

[0064] According to the ratio of 1:10 between the quality of walnut kernels in g and the volume ratio of ethanol aqueous solution in mL, 500 g of commercially available walnut kernels with inner testa (about 10% broken kernel rate) and 5 L of 10% volume concentration Mix the ethanol solution, adjust the pH of the system to 4.5 with 1M citric acid, raise the temperature of the system to 50°C, and add the compound enzyme of Novozymes at the ratio of 0.1U / g and 0.2U / g walnut kernel respectively SMASH and Max, put the mixed system in an ultrasonic cleaning machine, adjust the ultrasonic power to 1000W according to the ratio of ultrasonic power to 2W / g walnut kernel, ultrasonically treat for 30min, and then filter with gauze to recover the walnut kernel and extract respectively. The walnut kernels were repeatedly leached twice under the same conditions as above, and the dephenolized...

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Abstract

The invention relates to a method for extracting polyphenol substances from semen juglandis with inner seed coats by the aid of combined enzyme and ultrasonic treatment. The method includes acquiring the semen juglandis with the seed coats; feeding the semen juglandis with the inner seed coats into ethanol aqueous solution according to a volume ratio of 1:3-1:15 to obtain mixtures, regulating the pH (potential of hydrogen) of the mixtures by the aid of food-grade citric acid or hydrochloric acid until the pH of the mixtures is 3.5-6.5 and then adding pectinase and / or cellulase compounds into the mixtures to obtain polyphenol extracted systems. The method has the advantages that the total weight of dry substances, which are obtained after freeze drying is carried out on semen juglandis polyphenol crude extract prepared by the aid of the method, at least accounts for 2% of the weight of the semen juglandis with the coats, and the contents of polyphenol effective substances in the semen juglandis polyphenol crude extract can reach 15% at least; the semen juglandis polyphenol crude extract further can be separated by the aid of macroporous adsorption resin and industrial chromatographs, and accordingly products such as ellagic acid, hydrolysable tannin and juglone can be prepared.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of oil crop processing. More specifically, the present invention relates to a method for extracting polyphenols from walnut kernels with inner testa by combining enzymes and ultrasonic treatment, and also relates to the application of the walnut polyphenol crude extract obtained according to the method. [0003] 【Background technique】 [0004] Walnut (Juglans regia L.) has another name called walnut, brother peach, is Juglandaceae Juglans plant, ranks first in "four big dry fruits", is a kind of excellent economic crop that has both medicine and food. Walnuts are rich in nutrients such as oil, protein and vitamins, and have various functions such as strengthening the stomach, nourishing the blood, moistening the lungs, nourishing the kidneys and nourishing the brain. It has the reputation of "longevity fruit", "longevity fruit", "puzzle fruit" and "beauty fruit". Modern studies have shown that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L25/00A23L33/105A23L5/30C11B1/10
CPCA23V2002/00C11B1/10A23V2250/2132
Inventor 张文斌蒋将李笑笑杨瑞金华霄赵伟
Owner JIANGNAN UNIV
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