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Application of juglone to preparation of medicament for treating autoimmune and inflammatory diseases

An autoimmune and juglone technology, applied in allergic diseases, blood diseases, sensory diseases, etc., can solve problems such as antigen signal stimulation

Inactive Publication Date: 2012-11-28
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the pathological mechanism of EAE / MS is not fully understood, but it is generally believed that EAE is an inflammatory disease of the central nervous system caused by autoimmune inflammation mediated by helper T cells (TH1 and TH17). Directed differentiation of T cells depends on the stimulation of different antigenic signals derived from dendritic cells

Method used

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  • Application of juglone to preparation of medicament for treating autoimmune and inflammatory diseases
  • Application of juglone to preparation of medicament for treating autoimmune and inflammatory diseases
  • Application of juglone to preparation of medicament for treating autoimmune and inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1.1 Construction method of EAE mouse model

[0022] (1) Experimental animals and sources

[0023] Thirty female wild-type C57BL / 6 pathogen-free (SPF grade) mice, 6-8 weeks old, weighing 18-20g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. and bred in the Experimental Animal Center of Tianjin Medical University , The mouse feeding environment is room temperature 20-25 ℃, relative humidity 40%-60%.

[0024] (2) Preparation of Antigen Adjuvant Emulsion

[0025] 100 μg of MOG35-55 polypeptide and 500 μg of inactivated Mycobacterium tuberculosis (Mycobacterium tuberculosis, purchased from Difco) were completely mixed with 100 μl of normal saline and 100 μl of Freund’s adjuvant (purchased from SIGMA), and emulsified.

[0026] (3) Experimental grouping

[0027] In order to study the drug effect of juglone in the mouse EAE animal model, the mice were randomly divided into four groups, with 8 mice in each group, two normal saline control ...

Embodiment 2

[0098] Mix 50g of juglone and 280g of starch evenly, make granules with starch slurry (take 220g of starch and make starch slurry with water), sieve, dry, and pack into capsules.

Embodiment 3

[0100] Mix 80g of juglone and 340g of starch evenly, make granules with starch slurry (take 210g of starch to make starch slurry with water), sieve, dry, add 6‰ magnesium stearate, mix well, press into tablets, and coat with film. .

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Abstract

The invention discloses an application of juglone to preparation of a medicament for treating autoimmune and inflammatory diseases. As proved by an experimental result, the juglone can be used for lowering the disease incidence, average clinical score, highest clinical score and total clinical scores of mouse experimental autoimmune encephalomyelitis, lowering the percentages of an IFN (Interferon)-gamma secretion-relevant cell Th1 and a 1L-17A secretion-relevant cell Th17 in spinal marrow of mouse experimental autoimmune encephalomyelitis in a CD4+T cell, inhibiting the proliferation of a cell T induced by MOG (Myelin Oligodendrocyte Glycoprotein) of the mouse experimental autoimmune encephalomyelitis, and lowering the expression of CD11b+ angtigen presentation cells CD83 and MHCII in the spleen of the mouse experimental autoimmune encephalomyelitis. The juglone for preparing a medicament for inhibiting an immune system related to the invention is expected to become a medicament for clinically treating autoimmune inflammatory diseases such as multiple sclerosis, acute disseminated encephalomyelitis and the like.

Description

technical field [0001] The invention belongs to the technical field of application of traditional Chinese medicines, and relates to the application of juglone, a monomer of traditional Chinese medicine, in the preparation of medicines for treating autoimmune diseases and nervous system inflammatory diseases. Background technique [0002] Walnut catalpa (Juglans mandshurica Maxim.) is a deciduous tree of Juglans family Juglans, which is a precious timber tree species and an important medicinal plant. Juglone (Juglone; Nucin) is also known as 5-hydroxy-1,4-naphthoquinone; 5-hydroxy-1,4-naphthalenedione. It is a hydroxynaphthoquinone compound isolated from the fresh root bark, branch bark and green peel of Juglans, and it is the main active substance in Juglans. Walnut peeling is an astringent medicine, which has certain curative effects on bacillary dysentery and bone tuberculosis. In recent years, a large number of studies have shown that juglone has biological activities s...

Claims

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Application Information

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IPC IPC(8): A61P37/06A61P27/02A61P25/00A61P13/12A61P21/00A61P1/16A61P25/28A61P21/04A61P37/02A61P7/00A61K31/122A61P7/06
Inventor 张荣信葛禛禛张凯薛振毅
Owner TIANJIN MEDICAL UNIV
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