Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis

A method of melanoma and method establishment, applied in the field of dynamic detection of human melanoma high metastasis model, cell subline and establishment and metastasis, can solve the problems that the exact mechanism is not very clear and restricts the progress of human melanoma metastasis mechanism

Inactive Publication Date: 2011-05-25
SHANGHAI INST OF ONCOLOGY
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exact mechanism of tumor metastasis is still not very clear, and tumor metastasis has always been a hot spot in oncology research.
Therefore, the relatively large limitations in the research in this field have restricted the progress of research on the mechanism of human melanoma metastasis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis
  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis
  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0097] In order to make the technical means, creative features, goals and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

[0098] 1. In vivo screening of high metastasis model of human melanoma SCID mice transplanted subcutaneously

[0099] 1.1 Materials

[0100] 1.1.1 Animals SCID mice (T and B cell functional defects) were provided by Shanghai Cancer Institute [production license number is SCXK (Shanghai) 2007-0001]. Rats aged 6-8 weeks, weighing 18-22g, male and female, the test and breeding strictly complied with the requirements of the SPF standard [use license number is SYXK (Shanghai) 2007-0001].

[0101] 1.1.2 Cells A375 melanoma cells were long-term preserved and passaged by Shanghai Cancer Institute according to conventional methods (acquired from the Cell Bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences in September 2000, cell number...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a highly metastatic model of human melanoma, a highly metastatic cell subline of the human melanoma, creation methods for the highly metastatic model and the highly metastatic cell subline, and the dynamic detection of metastasis. The subcutaneously-transplanted mouse highly metastatic model and the corresponding cell subline are established in an in-vivo screening way in a mouse with severe combined immune deficiency (SCID) by using mouse lung metastasis, namely human malignant melanoma cell strain A375 pulmonary metastasis, wherein the highly metastatic cell subline of the human melanoma is A375sci, and has a human tumor cell karyotype; and 60 to 75 hypo-triploid-dominated chromosomes are acrocentric and have a heteroploid karyotype. The cell subline has the two routes of metastasis of blood trails and lymph. The in-vivo screening of the highly-metastatic model is performed by using animals with severe immune deficiency, and is expressed and applied in nude mice. A method for detecting Alu genes by using a polymerase chain reaction (PCR) method is simple, highly sensitive and highly specific, and can be used for detecting organ metastasis, particularly micrometastasis, in a human tumor animal-xenotransplantation model.

Description

technical field [0001] The invention relates to a high-metastasis model of human melanoma, a cell subline, a method for establishing it, and dynamic detection of metastasis, and belongs to the technical field of animal models and animal cell lines. Background technique [0002] Tumor invasion and metastasis are one of the most basic biological characteristics of malignant tumors. Death from primary tumors is relatively rare, and more than 90% of tumor patients die from the metastasis of tumor cells in the human body. [0003] Human melanoma is one of the main diseases that harm the head and neck, becoming the most common malignant tumor. However, the exact mechanism of tumor metastasis is not very clear at present, and tumor metastasis has always been a hot spot in oncology research. Therefore, the relatively large limitations in the research in this field have restricted the progress of the research on the mechanism of human melanoma metastasis. Contents of the invention...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/027C12N5/09C12Q1/68
Inventor 姚明王晓敏闫明霞孔韩卫刘蕾萨冰清李静
Owner SHANGHAI INST OF ONCOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com