Method for preventing serum interference and improving storage stability of reagent of anti-phospholipid antibody

An anti-phospholipid antibody and antigen-antibody technology is applied in the field of preventing serum interference and improving the storage stability of anti-phospholipid antibody assay reagents, and can solve the problems of serum interference and inability to obtain assay results.

Active Publication Date: 2012-07-11
SEKISUI MEDICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, although such sensitizers are excellent in promoting antigen-antibody reactions, accurate measurement results cannot be obtained with some samples.
Specifically, when the specimen is serum, by changing the components contained in the serum, a phenomenon called serum interference that adversely affects the measurement occurs, and accurate measurement results cannot be obtained.

Method used

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  • Method for preventing serum interference and improving storage stability of reagent of anti-phospholipid antibody
  • Method for preventing serum interference and improving storage stability of reagent of anti-phospholipid antibody
  • Method for preventing serum interference and improving storage stability of reagent of anti-phospholipid antibody

Examples

Experimental program
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Effect test

Embodiment 2

[0101] (1-1) In the preparation of the first reagent, 0.70% by weight of Lipitor D03 neutralized with NaOH was added to a phosphate buffer solution containing 1% bovine serum albumin (LotA: manufactured by Serologicals Co., Ltd.) (manufactured by NOF Corporation, molecular weight: 1,000,000), except that the first reagent was prepared, and an antiphospholipid antibody measurement reagent was prepared in the same manner as in Example 1.

Embodiment 3

[0103] (1-1) In the preparation of the first reagent, 0.45% by weight of Lipitor D05 neutralized with NaOH was added to a phosphate buffer solution containing 1% bovine serum albumin (LotA: manufactured by Serologicals Co., Ltd.) (manufactured by NOF Corporation, molecular weight: 1,000,000), except that the first reagent was prepared, and an antiphospholipid antibody measurement reagent was prepared in the same manner as in Example 1.

Embodiment 4

[0123] (1-1) In the preparation of the first reagent, in the phosphate buffer containing 1% bovine serum albumin (LotB), 0.66% by weight of Lipitor D03 (NOF Co., Ltd.) neutralized with NaOH was added. Preparation), except that, in the same manner as in Example 1, an antiphospholipid antibody assay reagent was prepared.

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Abstract

It is an object of the present invention to provide a method for preventing serum interference for assaying anti-Treponema pallidum antibodies and for improving storage stability of reagent of anti-phospholipid antibodies, which is excellent in long-term storage stability and enables an accurate diagnosis of syphilitic infection by preventing an occurrence of serum interference. The step of assaying anti-Treponema pallidum antibodies comprises a process that a sample is contacted with the anti-Treponema pallidum antibody reagent having an insoluble carrier supporting an anti-Treponema pallidum antigen thereon, a process that the insoluble carrier supporting an anti-Treponema pallidum antigen thereon is reacted with the anti-Treponema pallidum antibody in the sample, and a process that agglutination level of the antigen-antibody reaction is determined by optical measurement or visual observation, wherein the anti-Treponema pallidum antibody assaying reagent is added with a copolymer having a segment derived from 2-methacryloyloxyethyl phosphorylcholine and a segment derived from a hydrophilic monomer.

Description

[0001] This application is a divisional application with the filing date of December 7, 2006, the application number 200680045554.7, and the title of the invention being antiphospholipid antibody assay reagent and anti-Treponema pallidum antibody assay reagent. technical field [0002] The present invention relates to a method for preventing serum interference and improving the storage stability of an antiphospholipid antibody assay reagent, and also relates to an antiphospholipid antibody assay reagent capable of accurately diagnosing syphilis infection and having excellent long-term storage stability, and Anti-Treponemal pallidum antibody assay reagent capable of preventing serum interference and enabling accurate diagnosis of syphilis infection. Background technique [0003] Treponema pallidum, the causative agent of syphilis, upon infecting an organism, produces antibodies against the pathogen and antibodies reactive with phospholipids. The antiphospholipid antibody assa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571
CPCG01N2469/20G01N33/571G01N2333/20G01N33/531G01N33/545G01N33/92
Inventor 赤峰隆之大田哲也李艳
Owner SEKISUI MEDICAL CO LTD
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