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Method of building specificity endothelial cell growth promoting anticoagulant surface

A technology of endothelial cells and construction methods, applied in the field of surface modification of biomaterials, can solve the problems of incomplete realization of normal endothelial tissue function, disordered growth of cells, limited anticoagulant performance, etc., and achieve excellent endothelial cell growth-promoting performance, Excellent anticoagulant properties, promoting adhesion and growth effects

Inactive Publication Date: 2017-09-08
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can significantly promote the adhesion and growth of endothelial cells, their anticoagulant properties are limited, and cells grow disorderly on the surface of the material, so the obtained endothelial cell layer cannot fully realize the function of normal endothelial tissue.

Method used

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  • Method of building specificity endothelial cell growth promoting anticoagulant surface
  • Method of building specificity endothelial cell growth promoting anticoagulant surface
  • Method of building specificity endothelial cell growth promoting anticoagulant surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 prepares the biological material that the width of band and interval are 5 μm

[0049] A method for constructing an anticoagulant surface that specifically promotes endothelial cell growth, specifically comprising the following steps:

[0050] 1) Preparation of polydopamine layer on the surface of biomaterials

[0051] Immerse the biological material in a dopamine solution with a pH of 8.0, fully self-polymerize for 12 hours, wash and dry, repeat three times, the concentration of the dopamine solution is 0.1mg / ml, and obtain the surface of the biological material with a polydopamine layer;

[0052] 2) Microcontact printing immobilization of diaminopolyethylene glycol on the surface of biomaterials

[0053] Add the bis(3-aminopropyl)-terminated polyethylene glycol solution dropwise on the surface of the strip-shaped micropatterned polydimethylsiloxane (PDMS) stamp, and the strip-shaped micropatterned polydimethylsiloxane N2 Dry, then fully contact the PDMS...

Embodiment 2

[0058] Embodiment 2 prepares the biological material that the width of band and interval are 25 μ m

[0059] A method for constructing an anticoagulant surface that specifically promotes endothelial cell growth, specifically comprising the following steps:

[0060] 1) Preparation of polydopamine layer on the surface of biomaterials

[0061] Immerse the biological material in a dopamine solution with a pH of 8.5, fully self-polymerize for 24 hours, wash and dry, repeat three times, the concentration of the dopamine solution is 1 mg / ml, and obtain the surface of the biological material with a polydopamine layer;

[0062] 2) Microcontact printing immobilization of diaminopolyethylene glycol on the surface of biomaterials

[0063] Add the bis(3-aminopropyl)-terminated polyethylene glycol solution dropwise on the surface of the strip-shaped micropatterned polydimethylsiloxane (PDMS) stamp, and the strip-shaped micropatterned polydimethylsiloxane N 2 Dry, then fully contact the P...

Embodiment 3

[0068] Example 3 Preparation of biomaterials with a width and interval of 50 μm of the strips

[0069] A method for constructing an anticoagulant surface that specifically promotes endothelial cell growth, specifically comprising the following steps:

[0070] 1) Preparation of polydopamine layer on the surface of biomaterials

[0071] Immerse the biological material in a dopamine solution with a pH of 8.5, fully self-polymerize for 20 hours, wash and dry, repeat three times, the concentration of the dopamine solution is 0.5 mg / ml, and obtain the surface of the biological material with a polydopamine layer;

[0072] 2) Microcontact printing immobilization of diaminopolyethylene glycol on the surface of biomaterials

[0073] Add the bis(3-aminopropyl)-terminated polyethylene glycol solution dropwise on the surface of the strip-shaped micropatterned polydimethylsiloxane (PDMS) stamp, and the strip-shaped micropatterned polydimethylsiloxane N 2 Dry, then fully contact the PDMS ...

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Abstract

The invention discloses a method of building specificity endothelial cell growth promoting anticoagulant surface, belongs to the technical field of biomaterial surface modification. The method comprises the steps of 1, preparation of poly-dopamine of biomaterial surface; 2, micro-contact printing of fixed bi-amino glycol of biomaterial surface; 3, fixing of chitosan and heparin of biomaterial surface; 4, fixing of REDV of biomaterial surface; the building method is beneficial to the control of adhesion growth behavior of endothelial cells and the achieving of the complete healing of endothelial tissue; when the anticoagulant surface is used for the endovascular implantation materials or surface modification of equipment, excellent endothelial cell growth promoting property and anticoagulant property can be attached at the same time; due to the formation of a solid complex coating by auto-polymerization of dopamine on all solid material surfaces, and the formed coating has a strong ability to react with amino-containing substances, the building method can be used for the surface modification of almost all of biomaterials and for the building of anticoagulant surface for promoting the growth of specificity endothelial cells.

Description

technical field [0001] The invention belongs to the technical field of surface modification of biological materials, and in particular relates to a method for constructing an anticoagulant surface that specifically promotes the growth of endothelial cells. Background technique [0002] Intravascular implantation of materials or devices plays a very important and key role in the treatment of cardiovascular diseases. However, after implantation of materials or devices, surface thrombosis and endothelial dysfunction due to changes in blood components and endothelial cell function often lead to implantation. fail. [0003] Based on the understanding of interfacial biological reactions between materials and human blood and endothelial cells, depositing inorganic coatings on the surface of materials, preparing polymer layers or immobilizing bioactive molecules can improve the blood compatibility of materials and endow materials with certain Endothelial formation ability, but thes...

Claims

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Application Information

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IPC IPC(8): A61L31/10A61L31/16A61L33/00
CPCA61L31/10A61L31/16A61L33/0011A61L2300/252A61L2300/412A61L2300/606A61L2420/02A61L2420/08C08L79/02C08L71/02C08L5/08C08L89/00
Inventor 潘长江刘涛龚韬王翎任
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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