Naked mouse endothelial ancestral cell angiogenesis promoting model

An endothelial progenitor cell and angiogenesis technology, which is applied in animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problems of far different drug use conditions and can not truly simulate the actual situation, and achieve the effect of promoting angiogenesis.

Inactive Publication Date: 2005-12-21
福建中医学院
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Problems solved by technology

[0006] Vascular endothelial cells are the main cell model for screening drugs related to angiogenesis. Although this model can study the mechanism of action of drugs at the cellular and molecular levels, because the research environment is in vitro, it is far from the actual clinical drug use and cannot be truly tested. Simulate the actual situation
[0007] Although studies have shown that endothelial progenitor cells are directly involved in the process of human angiogenesis, there is currently no in vivo model of endothelial progenitor cells for research and screening of drugs related to angiogenesis

Method used

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Embodiment Construction

[0011] The technical scheme of the present invention is as follows: endothelial progenitor cells are mixed with matrigel and injected into the subcutaneous tissue of nude mice, 1ml of matrigel contains 10 2 -10 6 For each endothelial progenitor cell, the injection volume is 10μL-10mL; Matrigel is liquid at 4°C, and Matrigel quickly forms a solid gel at the body temperature of nude mice. Since Matrigel provides a more natural microenvironment to stimulate vascular responses, it can induce and (or) maintain the differentiation of various cells, and administer drugs to nude mice in a conventional manner, and the drugs given are the drugs studied, and there is no specific Restriction, so that the drug affects the differentiation of endothelial progenitor cells in matrigel in nude mice; endothelial progenitor cells differentiate into vascular endothelial cells with significant structural characteristics in matrigel, and grow out a network-like vascular structure; through convention...

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Abstract

The invention involves a model of naked mouse endothelial ancestral cell which urges the new born of blood vessel. The endothelial ancestral cell after being mixed with the base jel is injected into the endothelial tissue of naked mouse. Give medicine through routine way to naked mouse to effect the differentiation of the endothelial ancestral cell inside the base-jel in naked mouse; the endothelial ancestral cell differentiates in the base jel into blood vessel ebdothelial cell with remarkable structure characteristic to growth blood vessel of network grid form. Then take out the base jel. Advantages: low cost and short period.

Description

Technical field: [0001] The invention relates to a biomedical research model. As an in vivo model to study and screen drugs related to promoting angiogenesis and their mechanism of action. Background technique: [0002] Currently, there are two types of established drug models for screening drugs related to angiogenesis: [0003] (1) In vivo model [0004] A commonly used in vivo model is chorioallantoic, but the indicators of this model are not standardized, and it is impossible to explain the mechanism of action of the drug from the molecular and cellular levels. [0005] (2) In vitro model [0006] Vascular endothelial cells are the main cell model for screening drugs related to angiogenesis. Although this model can study the mechanism of action of drugs at the cellular and molecular levels, because the research environment is in vitro, it is far from the actual clinical drug use and cannot be truly tested. Simulate real situations. [0007] Although studies have sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 陈可冀高冬廖联明宋军林久茂郑良朴杜建
Owner 福建中医学院
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