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35 results about "Research environment" patented technology

Research protocol toolkit

Exemplary embodiments of the present invention comprise a tool kit for the design and execution of research protocols, for example, comprising a web-based research protocol composer for generating a data processing protocol; a collector for collecting data entered via an internet based questionnaire; a database for data; and a protocol processing routine for processing data and generating an output. For example, the toolkit suitably serves as a “hub” of a global research environment (e.g., connected via the Internet or other network) that creates and connects communities of researchers and facilitates the sharing of software and results. Additionally, the present invention provides standardized and custom online “programs” or “scripts” (referred to herein as “protocols”) that define and guide events in experiments, clinical trials, surveys, and other kinds of studies. These protocols are created and executed online.
Owner:PASADERO

Synthesis monitoring system for water quality mutation and monitoring method thereof

The invention discloses an integrated monitoring system of water quality sudden changes and a monitoring method thereof. The monitoring system comprises a water quality physical and chemical index analyzing system, a creature behavior index analyzing system and a water quality condition analyzing system realized on the two analyzing systems, wherein the water quality physical and chemical index analyzing system and the creature behavior index analyzing system are in a parallel state, and obtained results are used as the basis for the water quality condition analyzing system to carry out water quality integrated analysis. The invention is combined with the analyzing technology of water quality physical and chemical indexes, realizes the integrated toxic analysis of water quality changes, realizes the data exchange of each system and the remote control and the data transmission of the entire system, realizes the early warning of unknown pollutant of the water body, and has an important directing function for researching the environmental pollution, particularly the sudden and severe accidental changes.
Owner:福州怡景环境技术有限公司

Indoor pseudo-satellite signal multipath microscopic parameter analysis system based on statistical model

The invention discloses an indoor pseudo-satellite signal multipath microscopic parameter analysis system based on a statistical model. A software system comprises an indoor structured scene modelingmodule, which is used for carrying out three-dimensional scene modeling with an office area as a target research environment, and is provided with a pseudo-satellite launcher and a pseudo-satellite data collection point; a multipath signal tracking module for carrying out multi-path signal tracking by adopting an image method; a multipath signal extraction module used for extracting multipath signals based on time and power judgment; and a data derivation and statistics analysis module used for matching a deterministic model obtained by inductive statistics with a theoretical model, and verifying the correctness of a multipath microcosmic parameter theoretical model suitable for a pseudo-satellite signal system. Based on the system disclosed by the invention, channel characteristics of pseudo-satellite multipath of a multipath delay extended model, a multipath power delay model and the like in indoor environments can be revealed, and the design of a pseudo-satellite receiver and a simulator is facilitated.
Owner:SOUTHEAST UNIV

Induction design plan for PCR-DGGE research on environment microorgan population

A prime design scheme for using PCR-DGGE to research the microbial community structure in environmental specimen includes such steps as choosing environmental specimen (agricultural field soil), extracting the genomic DNAs of all microbes, purifying for using them as template of PCR amplification, choosing universal prime pair for prokaryotic 16 SrRNA gene amplification, adding GC hairpin structure to terminal 5', PCR amplifying, electrophoretic separating in DGGE, and observing if the PCR resultant is fully separated.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Video denoising method based on actual camera noise modeling

The invention discloses a video denoising method based on actual camera noise modeling. The method comprises the following specific steps: (1) exploring the physical cause of main noise in an imaging process and establishing a mathematical model of noise distribution; (2) on the basis of the established noise model, carrying out model parameter calibration, and generating a noise video training set conforming to reality; (3) designing a video denoising and enhancement neural network, and combining space and time information to suppress and weaken the noise; and (4) training and optimizing the neural network, and verifying the practicability of the method by using the synthesized and actually acquired video data set. The de-noising method is suitable for de-noising weak light videos and has very important application requirements in the fields of national defense military affairs, security and protection monitoring, scientific research environment protection and the like.
Owner:NANJING UNIV

Automatic archive compiling and researching device

The invention belongs to the technical field of archive classification and retrieval, and relates to an automatic archive compiling and researching device based on an archive management information system of B / S architecture. The device is formed by allowing a client browser, an archive information input and management module, an archive compiling and researching module and databases to be communicated through electrical information, wherein the archive information input and management module comprises three functional modules of the inner file list management module, the file list management module and the specialist registration card information module, the archive compiling and researching module is composed of three functional sub-modules of the archive classification compiling and researching sub-module, the file word size index compiling and researching sub-module and the specialist information compiling and researching sub-module, the databases are composed of the file list database, the inner file list database, the archive classification database, the specialist basic information registration database, the specialist thesis detail database, the specialist project detail database and the archive compiling and researching foundation database, and the client browser is any browser software running on a networked common computer or terminal equipment. The design principle is scientific and reliable, the compiling and researching labor intensity is small, working efficiency is high, information omission is small, and the compiling and researching environment is friendly.
Owner:QINGDAO UNIV

Coal rock solid gas coupling fine observation mechanics loading device

The invention discloses a solid and gas coupling mesomechanics charger of coal rocks, which has the following structure: a testing body is opened with a testing cavity, a measuring orifice and a multi-purpose air vent, a transparent sight glass is fixed at an opening position of the testing cavity, a measuring conducting wire is arranged inside the measuring orifice, an inner port of the multi-purpose air vent is positioned on an inner wall of the testing cavity, and an outer port of the multi-purpose air vent is connected with a gas pipe; a cushion block, a limit pressure head and a loading head are arranged inside the testing cavity, an upper part of the cushion block is opened with an L-shaped nick, a vertical surface of the L-shaped nick is over against the limit pressure head that is fixed at an inner end of a limit rod, and a transverse surface of the L-shaped nick is over against the loading head that is fixed at a lower end of a loading rod. The solid and gas coupling mesomechanics charger can implement not only the uniaxial mechanics test under the solid and gas coupled environment but also the plane strain mechanics test under the solid and gas coupled environment, provides reliable testing conditions for the solid and gas coupling mesomechanics test that collects image signals and acoustic emission signals that are inspected microscopically in real time, and leads the research environment of the coal and gas prominent mechanism test to be closer to real mine environment.
Owner:CHONGQING UNIV

Interactive Analysis and Reporting System for Telecom and Network Industry Data

InactiveUS20100153172A1Easy accessFinanceMarket data gatheringDate RangeNational data
This invention relates to a method and system for providing a subscriber with secure access to a custom market share research environment for viewing quantitative market share data stored in a database on the server, and for generating real-time customized interactive reports of said data based parameters such as quarterly data, yearly data, date-range presented data, individual company data, market segment data, entire market data, geographical region data, country data, revenue data, units data, and user-defined data.
Owner:MOTA RAY

Sand flow sediment transportation monitoring method

The invention relates to a sand flow sediment transportation monitoring method, and a device adopted in the method is a trapezoidal sand sampler. The sand sampler comprises a sand collection passage, a sand guide passage, a sand storage box, a fixing plate, plugging screws of the sand storage box, and a seal cover. The trapezoidal sand sampler in the method is used for measuring indoor and outdoor sand transportation rate of sand flow and indoor and outdoor sand flow structures, collected sand can be taken out on the spot without the need of opening the sand sampler, and the sand sampler has the advantages of portability and convenience for operation, can improve the working efficiency and reduce the labor intensity, is applicable to areas of wide range, can be used at an arid area and a wet area, can be widely used for researching environment, ecology, aeolian landform, sand-proof engineering and other aspects.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI

Experiment device and experiment method for in-situ spectroscopy joint application of Mars

The invention relates to an experiment device and an experiment method for in-situ spectroscopy joint application of Mars. The experiment device comprises a Mars environment simulating experiment device for simulating the surface environments of Mars, and an in-situ spectroscopy joint application experiment device for in-situ spectroscopy testing under the Mars environment, wherein the Mars environment simulating experiment device comprises a vacuum cabin, a vacuum control unit, a humidity control unit, a first gas supply unit, and a temperature control unit, the vacuum control unit is connected with the vacuum cabin, the humidity control unit is communicated with the vacuum cabin, the first gas supply unit is communicated with the humidity control unit, and the temperature control unit is connected with the vacuum cabin; the in-situ spectroscopy joint application experiment device is arranged at the exterior of the vacuum cabin, and is used for remote Raman spectrum and remote LIBS spectroscopy joint application testing. The experiment device has the advantages that the Mars environments can be simulated, such as temperature, humidity and atmosphere, the spectroscopy of mineral samples under the Mars environment can be performed with in-situ testing, the influence to the spectroscopy of mineral samples by the environment parameters is studied, and the single variable and multiple variables of the mineral content can be quantitatively analyzed and studied.
Owner:SHANDONG UNIV

Detoxification method for excised stem segment of grape

The invention relates to a detoxification method for an excised stem segment of grape. The method comprises the following steps: 1) selecting a grape variety and a test-tube plantlet related to parental stock, wherein the grape variety is subjected to a secondary culture until a stem is semi lignified; under a sterile condition, clipping a single bud stem segment and inoculating in a culture media, followed by placing in a 2000-3000 lx culture room having a temperature of 22-28 DEG C until a white rootlet is exposed to obtain a excised stem segment of the grape; 2) placing the excised stem segment of the grape in a incubator having a temperature of 35-40 DEG C, followed by carrying out a dark treatment while carrying out a heat treatment; 3) observing a growth condition of the lateral bud after heat treating for 10 days, visual peeling 0.2-0.8 mm of the micro stem apex under a sterile condition when a length of the yellow young shoot is 6-10 cm, then placing the micro stem apex in the culture media to obtain the propagation material. According to the present invention, illumination and frequent temperature change process do not require, such that the method has characteristics of simple operation, time saving, electricity saving, labor saving, high efficiency and low cost. In addition, the method has not strict requirements on research environments and research conditions and facilities, such that the method can be applicable for the research and teaching unit with the tissue culture condition.
Owner:CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI

High-precision temperature sensor supporting NB-IoT

The invention provides a high-precision temperature sensor supporting NB-IoT and relates to a high-resolution, high-precision, high-stability and low-power consumption temperature sensor which can beapplied in industrial and scientific research environments and is convenient for mass interconnection, and long-distance signal transmission and sharing. The high-precision temperature sensor comprises a quartz crystal probe (1), a satellite navigation system antenna (2), a satellite navigation system module (3), an ASIC (4), an NB-IoT chip (5), an NB-IoT antenna (6), a lithium titanate rechargeable battery (7), an ABS engineering plastic case and a silicone rubber waterproof cover; the high-precision temperature sensor can work in the environment of from minus 50 DEG C to 60 DEG C above freezing, achieve the protection grade IP67, completely prevent dust from entering and withstand water in a short time (1m); the power consumption is low, and the two lithium titanate rechargeable batteries can work for 4 years; and the high-precision temperature sensor can shares with other users, control centers and display centers of NB-IoT via an NB-IoT base station and a cloud database, is high-resolution, high-precision, high-stability and low-power consumption, supports the Internet of Things, and is convenient for mass interconnection and long-distance transmission of temperature signals.
Owner:章礼道

Artificial intelligence reinforcement learning service platform

ActiveCN111427549AReliable Cloud Integrated Development EnvironmentSecure Cloud Integrated Development EnvironmentSoftware testing/debuggingSoftware designCloud processingEngineering
The invention relates to an artificial intelligence reinforcement learning service platform. The system is structurally divided into an infrastructure layer, an application service layer and an interface access layer, the infrastructure layer provides network resources, computing resources, storage resources and virtualization service resources required by the reinforcement learning service platform, and provides IT infrastructure services related to cloud storage and cloud processing through virtualization, load balancing, disaster recovery backup and elastic computing technologies; the application service layer comprises a Project-based packaging and management module, a cloud development and debugging environment module and a virtual development environment interface module; the three systems provide various universal or self-defined cloud research environments for field researchers from top to bottom; the platform encapsulates data, algorithms and research environments involved inreinforcement learning research in a virtualization container in a Project form, and opens up an independent test environment for each user using the platform; and the interface access layer can enable the reinforcement learning researcher to manage the cloud computing environment in a self-service manner.
Owner:COMP NETWORK INFORMATION CENT CHINESE ACADEMY OF SCI

Cooperative operation test platform capable of supporting basic research on digital twin fully mechanized coal mining face

PendingCN114004103AAchieve high-precision reproductionImplement preselection planningDesign optimisation/simulationSpecial data processing applicationsBasic researchProcess engineering
The invention relates to a collaborative operation test platform capable of supporting basic research of a digital twin fully-mechanized coal mining face. The collaborative operation test platform comprises a fully-mechanized coal mining face three-machine multifunctional digital test platform, a fully-mechanized coal mining face three-machine collaborative operation visual virtual test simulation system and a digital twin data and fully-mechanized coal mining face three-machine test platform intelligent service system. Operation reproduction, mining rehearsal and fault early warning are carried out on the mining process of the fully mechanized coal mining face, real-time interaction between a virtual prototype test platform and a physical prototype test platform is achieved, common underground engineering problems can be researched, and a research environment and platform support are created for researching application of some new technologies.
Owner:TAIYUAN UNIV OF TECH

Adhesive composite wear-resistant super-hydrophobic coating and preparation method thereof

The invention discloses an adhesive composite wear-resistant super-hydrophobic coating and a preparation method thereof. The adhesive composite wear-resistant super-hydrophobic coating is obtained by coating a rigid substrate with an adhesive and then pressing in nano silicon dioxide. The rigid substrate is glass. The adhesive is one or more of silicone rubber, water-based paint, water-based adhesive sticker, 502 glue and epoxy resin AB glue. The prior art generally has the problems of environmental pollution, complex process, high cost and the like, cannot realize large-scale preparation, and is mostly limited in an ideal research environment of a laboratory. According to the invention, HMDS is used as a modifier, super-hydrophobic SiO2 particles are prepared under a fluorine-free condition, an adhesive is introduced as an auxiliary coating, the composite super-hydrophobic coating with high mechanical strength is prepared, and the adhesive, a preparation process and a wear resistance enhancement mechanism are analyzed and researched.
Owner:SUZHOU UNIV

Semiconductor refrigeration thermo-electric generation experimental instrument

The invention discloses a semiconductor refrigeration thermo-electric generation experimental instrument comprising a semiconductor refrigerator and a main machine. The semiconductor refrigerator is internally provided with electric heating wires, a thermometer, an in-machine cooling fan and an overheat protector, wherein all parts in the semiconductor refrigerator are smartly combined with all unit circuits in the main machine, conversion switches, and the like to form a physical experimental instrument capable of carrying out quantitative experimental projects, such as research on a semiconductor refrigeration coefficient, research on a thermo-electric generation rule, measurement of the internal resistance of a thermo-electric power supply, and the like and fill the gap of the prior art. The invention has the advantages of convenient and reliable use and high performance price ratio, can guide university students to research environment-friendly refrigeration and environment-friendly generation and cultivate a concept of researching new energy and can be used in common physical experiments, modern physical experiments and college physical experiment courses in colleges and universities or used as a physical experiment demonstration instrument.
Owner:SHANGHAI MUNICIPAL ELECTRIC POWER CO +1

Method for predicting common environment poison property/activity on the basis of intelligent correlation index

The invention relates to a method for predicting common environment poison property / activity on the basis of an intelligent correlation index, which is suitable for predicting the poison property / activity according to the molecular structure information of environment poison compound. The method comprises the following steps: (1) defining an atom type, and dividing the atom into four classes according to the branching coefficient of the atom; (2) defining the property correlative parameter (PCP) and the distance correlative function (DCF) of a non-hydrogen atom by a user according to the structural characteristic of the researched environment poison module; (3) determining an intelligent correlative function; (4) calculating an intelligent correlative index (ICI); and (5) and obtaining anoptimal prediction model according to a statistical result. Experiments prove that the method for representing the molecular structure of the environment poison has the advantages of specific materialization meaning, strong structural representation capability and good expansion performance and can be regulated, and good effect is obtained in the quantitative structure activity prediction research of various types of environment poison modules.
Owner:SHAANXI UNIV OF SCI & TECH

Potential interviewers recruitment and selection system, market research conducting method, and mobile electronic device

A potential interviewers recruitment and selection system for market research is disclosed. A method of conducting market research via passersby interview and a mobile electronic device is also described. The system, method and device work together in order to incorporate high-tech combined with the human factor to obtain reliable data and focus on research environment. In the market research conducting method the electronic device carrier goes into the field to interview passersby, delivering the collected data to a central server.
Owner:LEAN SURVEY PESQUISAS DE MERCADO LTDA

Cadmium (Cd) strongly-induced promoter CdS2 and application thereof

The invention discloses a segmented plant gene sequence. According to the segmented plant gene sequence, a cadmium (Cd) strongly-induced rice specific gene promoter CdS2 is separated, cloned and identified from Oryza sativa L cv.Nipponbare, and acquired transgenic rice is subjected to cadmium (Cd) treatment and is subjected to GUS chemical dyeing to find that each part of treated transgenic plants is significantly dyed; and moreover, by analyzing Gus gene expression quantities before and after RT-qPCR identification treatment, results show that the Gus gene expression quantity of the transgenic plants after being subjected to cadmium (Cd) treatment is 887.9 times that of the transgenic plants before being subjected to cadmium (Cd) treatment and can reach 4 times of ACTIN activity of a constitutive promoter. The promoter disclosed by the invention can be used for reflecting the cadmium pollution strength in soil according to the expression quantity of the promoter in rice, has the characteristics of simplicity in operation, low detection limit, micro sensitivity and high accuracy, can play important significance in researching environment relationships, improving ecological environments and achieving sustainable development, can also be used for actively analyzing pollution formation principles, and ensures that valuable experiences for avoidance and controllable transformation of cadmium pollution can be further provided.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI

Method for predicting common environment poison property/activity on the basis of intelligent correlation index

The invention relates to a method for predicting common environment poison property / activity on the basis of an intelligent correlation index, which is suitable for predicting the poison property / activity according to the molecular structure information of environment poison compound. The method comprises the following steps: (1) defining an atom type, and dividing the atom into four classes according to the branching coefficient of the atom; (2) defining the property correlative parameter (PCP) and the distance correlative function (DCF) of a non-hydrogen atom by a user according to the structural characteristic of the researched environment poison module; (3) determining an intelligent correlative function; (4) calculating an intelligent correlative index (ICI); and (5) and obtaining anoptimal prediction model according to a statistical result. Experiments prove that the method for representing the molecular structure of the environment poison has the advantages of specific materialization meaning, strong structural representation capability and good expansion performance and can be regulated, and good effect is obtained in the quantitative structure activity prediction research of various types of environment poison modules.
Owner:SHAANXI UNIV OF SCI & TECH

Intelligent experiment center management method

The invention relates to an intelligent experiment center management method, which comprises the steps of reserving a laboratory, applying materials, preparing experiment consumables in place in advance, orderly updating inventory, reasonably distributing instruments and ensuring orderly instrument management; after the user arrives, enabling the access control module to acquire a current user identifier and match the current user identifier with the reservation information, and allowing the user to pass when information is successfully matched, thereby ensuring personnel management; the method also includes that a user obtains consumables from a designated position; after the door of the laboratory is closed, the safety supervision module is started, the safety supervision module continuously monitors before the user leaves the experiment center, corresponding processing is carried out when an abnormality is monitored, so the safety of the laboratory is ensured; and after the user leaves, the safety supervision module continuously works for a period of time and then is closed, a laboratory management record is generated, and the experiment center management is safe and can be examined. Management work is converted from extensive management to refined, safe and intelligent management, investment of resources such as manpower, material resources and financial resources is reduced, the management level is improved, and a visual, safe and intelligent scientific research environment is created.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY

Cadmium strongly inducible promoter cds2 and its application

The invention discloses a plant gene sequence. The present invention isolates, clones and identifies a rice-specific gene promoter Cd S2 that is strongly induced by cadmium (Cd) from Nipponbare rice, and treats the obtained transgenic rice with cadmium (Cd). It is found through GUS chemical staining that after the treatment The transgenic plants had obvious staining in various parts. And through the RT-qPCR analysis of the expression of Gus gene before and after treatment, it was found that after cadmium (Cd) treatment, the expression of Gus gene in transgenic plants was 887.9 times that of before treatment, reaching 4 times the activity of the constitutive promoter ACTIN. The promoter can be used to reflect the intensity of cadmium pollution in soil through its expression level in rice. Its characteristics of simple operation, low detection limit, trace sensitivity and high accuracy are of great significance to the study of environmental relations and the improvement of ecological environment. , play an important role in sustainable development, and can actively analyze the principles of pollution formation, and further provide valuable experience for their avoidance and controllable transformation.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI

An artificial intelligence reinforcement learning service platform

ActiveCN111427549BReliable Cloud Integrated Development EnvironmentSecure Cloud Integrated Development EnvironmentSoftware testing/debuggingSoftware designCloud processingEngineering
The invention relates to an artificial intelligence reinforcement learning service platform, which is structurally divided into an infrastructure layer, an application service layer, and an interface access layer. The infrastructure layer provides network resources, computing resources, storage resources and virtual resources required by the reinforcement learning service platform. Service resources, and provide IT infrastructure services related to cloud storage and cloud processing through virtualization, load balancing, disaster recovery and elastic computing technologies; the application service layer includes Project-based packaging and management modules, cloud development and debugging environment modules , virtual development environment interface module; the three parts provide various general or customized cloud research environments for researchers in the field from top to bottom; the platform encapsulates the data, algorithms and research environments involved in reinforcement learning research in the form of Project In the virtualization container, an independent experimental environment is opened up for each user of the platform; the interface access layer enables reinforcement learning researchers to self-manage their cloud computing environment.
Owner:COMP NETWORK INFORMATION CENT CHINESE ACADEMY OF SCI

Fluidic system and related methods

Fluidic systems including cartridges with modular components (cassettes) and / or microfluidic channels for performing chemical and / or biological analyses are provided. The systems described herein include a cartridge comprising, in some embodiments, a frame, one or more cassettes which may be inserted into the frame, and a channel system for transporting fluids. In certain embodiments, the one or more cassettes comprise one or more reservoirs or vessels configured to contain and / or receive a fluid (e.g., a stored reagent, a sample). In some cases, the stored reagent may include one or more lyospheres. The systems and methods described herein may be useful for performing chemical and / or biological reactions including polymerase chain reactions (PCR) such as those performed within a laboratory, clinical (e.g., hospital), or research setting.
Owner:ARCHERDX

A file automatic compilation and research device

The invention belongs to the technical field of archive classification and retrieval, and relates to an automatic archive compiling and researching device based on an archive management information system of B / S architecture. The device is formed by allowing a client browser, an archive information input and management module, an archive compiling and researching module and databases to be communicated through electrical information, wherein the archive information input and management module comprises three functional modules of the inner file list management module, the file list management module and the specialist registration card information module, the archive compiling and researching module is composed of three functional sub-modules of the archive classification compiling and researching sub-module, the file word size index compiling and researching sub-module and the specialist information compiling and researching sub-module, the databases are composed of the file list database, the inner file list database, the archive classification database, the specialist basic information registration database, the specialist thesis detail database, the specialist project detail database and the archive compiling and researching foundation database, and the client browser is any browser software running on a networked common computer or terminal equipment. The design principle is scientific and reliable, the compiling and researching labor intensity is small, working efficiency is high, information omission is small, and the compiling and researching environment is friendly.
Owner:QINGDAO UNIV

Artificial intelligence reinforcement learning service system

The invention discloses an artificial intelligence reinforcement learning service system, and particularly relates to the field of reinforcement learning development platforms, and the system comprises an interface access layer, a service layer, a function layer, a storage layer, an arrangement layer, a model layer and a buffer layer. The interface access layer is an access layer of a cloud service consumer and enables a reinforcement learning researcher to manage a cloud computing environment in a self-service manner; the service layer comprises three parts of a Project-based packaging and management module, a cloud development and debugging environment module and a virtual development environment interface module; the three parts provide various universal or self-defined cloud research environments for field researchers from top to bottom; according to the platform, data, an algorithm and a research environment related to reinforcement learning research are packaged in a virtualization container in a Project form, and an independent test environment is developed for each user using the platform. According to the invention, a visual image of data is provided, code writing and debugging previewing can be carried out at the same time, the effect can be checked in real time, and a researcher can analyze and verify an algorithm.
Owner:深圳市优服网技术服务有限公司

Detoxification method for excised stem segment of grape

The invention relates to a detoxification method for an excised stem segment of grape. The method comprises the following steps: 1) selecting a grape variety and a test-tube plantlet related to parental stock, wherein the grape variety is subjected to a secondary culture until a stem is semi lignified; under a sterile condition, clipping a single bud stem segment and inoculating in a culture media, followed by placing in a 2000-3000 lx culture room having a temperature of 22-28 DEG C until a white rootlet is exposed to obtain a excised stem segment of the grape; 2) placing the excised stem segment of the grape in a incubator having a temperature of 35-40 DEG C, followed by carrying out a dark treatment while carrying out a heat treatment; 3) observing a growth condition of the lateral budafter heat treating for 10 days, visual peeling 0.2-0.8 mm of the micro stem apex under a sterile condition when a length of the yellow young shoot is 6-10 cm, then placing the micro stem apex in theculture media to obtain the propagation material. According to the present invention, illumination and frequent temperature change process do not require, such that the method has characteristics of simple operation, time saving, electricity saving, labor saving, high efficiency and low cost. In addition, the method has not strict requirements on research environments and research conditions and facilities, such that the method can be applicable for the research and teaching unit with the tissue culture condition.
Owner:CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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