35 results about "Research environment" patented technology

The invention relates to a method for predicting common environment poison property / activity on the basis of an intelligent correlation index, which is suitable for predicting the poison property / activity according to the molecular structure information of environment poison compound. The method comprises the following steps: (1) defining an atom type, and dividing the atom into four classes according to the branching coefficient of the atom; (2) defining the property correlative parameter (PCP) and the distance correlative function (DCF) of a non-hydrogen atom by a user according to the structural characteristic of the researched environment poison module; (3) determining an intelligent correlative function; (4) calculating an intelligent correlative index (ICI); and (5) and obtaining anoptimal prediction model according to a statistical result. Experiments prove that the method for representing the molecular structure of the environment poison has the advantages of specific materialization meaning, strong structural representation capability and good expansion performance and can be regulated, and good effect is obtained in the quantitative structure activity prediction research of various types of environment poison modules.

The invention discloses a plant gene sequence. The present invention isolates, clones and identifies a rice-specific gene promoter Cd S2 that is strongly induced by cadmium (Cd) from Nipponbare rice, and treats the obtained transgenic rice with cadmium (Cd). It is found through GUS chemical staining that after the treatment The transgenic plants had obvious staining in various parts. And through the RT-qPCR analysis of the expression of Gus gene before and after treatment, it was found that after cadmium (Cd) treatment, the expression of Gus gene in transgenic plants was 887.9 times that of before treatment, reaching 4 times the activity of the constitutive promoter ACTIN. The promoter can be used to reflect the intensity of cadmium pollution in soil through its expression level in rice. Its characteristics of simple operation, low detection limit, trace sensitivity and high accuracy are of great significance to the study of environmental relations and the improvement of ecological environment. , play an important role in sustainable development, and can actively analyze the principles of pollution formation, and further provide valuable experience for their avoidance and controllable transformation.

The invention relates to a detoxification method for an excised stem segment of grape. The method comprises the following steps: 1) selecting a grape variety and a test-tube plantlet related to parental stock, wherein the grape variety is subjected to a secondary culture until a stem is semi lignified; under a sterile condition, clipping a single bud stem segment and inoculating in a culture media, followed by placing in a 2000-3000 lx culture room having a temperature of 22-28 DEG C until a white rootlet is exposed to obtain a excised stem segment of the grape; 2) placing the excised stem segment of the grape in a incubator having a temperature of 35-40 DEG C, followed by carrying out a dark treatment while carrying out a heat treatment; 3) observing a growth condition of the lateral budafter heat treating for 10 days, visual peeling 0.2-0.8 mm of the micro stem apex under a sterile condition when a length of the yellow young shoot is 6-10 cm, then placing the micro stem apex in theculture media to obtain the propagation material. According to the present invention, illumination and frequent temperature change process do not require, such that the method has characteristics of simple operation, time saving, electricity saving, labor saving, high efficiency and low cost. In addition, the method has not strict requirements on research environments and research conditions and facilities, such that the method can be applicable for the research and teaching unit with the tissue culture condition.