DNA vaccine for promoting regeneration and functional rehabilitation of nerves of central system

Abstract

The invention provides a DNA vaccine for promoting regeneration and functional rehabilitation of nerves of a central system. The vaccine consists of antigen genes and eukaryon expression vectors, wherein the antigen genes are fusion genes which are formed by linearly connecting gene fragments in the LRR structure field of the coding LINGO-1, gene fragments in the EGFL structure field of the coding TN-R and gene fragments of the 1011st to the 1271st amino acid of the coding neurocan in sequence; and two of the gene fragments are connected by the DNA sequences coding three alanine. The DNA vaccine can express the fusion genes of the LRR structure field of the LINGO-1, the EGFL structure field of the TN-R and the 1011st to the 1271st amino acid of the neurocan, stimulate the human body to generate various antibodies of the nerve regeneration inhibiting factor, resist the activity of the axon growth inhibiting factor, and provide a good microenvironment for the regeneration and the functional rehabilitation of the nerves of the central system.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to medical preparations, especially medical preparations containing antigens. Background technique [0002] Nerve regeneration and repair after central nervous system (Central Nervous System, CNS) injury is a very complex pathophysiological process, involving changes at all levels from molecules, cells, organs to the whole. The current research shows that the axon of the central nervous system is not lack of regenerative ability, but the internal environment of the central nervous system is not suitable for regeneration. In the early stage of CNS injury, the damaged and disintegrated myelin sheath (formed by oligodendrocytes) releases a large number of myelin-associated axon growth inhibitors, such as Nogo-A and myelin-associated glycoprotein (MAG) , oligodendrocyte myelin glycoprotein (oligodendrocyte-myelinglycoprotein, OMgp), etc. [0003] The current methods of counteracting th...

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Problems solved by technology

Although the use of IN-1 to block Nogo-A is an attractive approach, there are many other inhibitory components in myelin, and IN-1 cannot block other numerous inhibitory molecules;

[0005] 2. Purified myelin was used as an immunogen for immunization; some scholars used purified myelin to immunize rats and successfully blocked all inhibitory factors. However, this method must use Freund’s incomplete adjuvant, which is not applicable to humans, and Potential risk of inducing a widespread autoimmune response;

[0006] 3. Construct a DNA vaccine against NgR or use prokaryotic expressed NgR protein as an antigen. Although this method is to counteract the key receptor molecules on the inhibitory signaling pathway, current studies have shown that there are other inhibitory signal bypasses, which is still unsatisfactory

[0007] It can be seen that the current methods or vaccines against the inhibitory effect of axon regeneration are mostly aimed at a single inhibitory factor, and it is difficult to achieve the ideal effect of promoting nerve regeneration and functional recovery in the central system

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