Biological material for brain injury renovation and its preparing process

A biomaterial, post-brain injury technology, applied in medical science, prostheses, coatings, etc., can solve the general tumor risk, cannot effectively control the release of antibodies and other problems, achieve good cell compatibility, and promote functional recovery. , the effect of good compatibility

Inactive Publication Date: 2007-03-14
首都医科大学北京神经科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to use this specific antibody has become a new technical problem: conventional drug routes cannot pass through the blood-brain barrier; the method of implanting antibody-producing hybrid tumor cells in the brain cannot effectively control the release of antibodies, and there are many problems. The risk of tumorigenesis; the purified antibody is delivered to the injury site through the pump implanted in the body, which can make the continuous supply of the antibody reach several weeks to several months, but after the antibody is used up, a second operation is required to remove the pump

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Preparation of Hyaluronic Acid Hydrogel

[0014] Prepare a hyaluronic acid solution with a mass percentage of 1% with deionized water, add 1M hydrochloric acid solution dropwise to the hyaluronic acid solution, and stir at the same time to fully mix the two until the pH value reaches 4.75; Hydrazide: hyaluronic acid = 1: 4 ~ 10" ratio Weigh adipate dihydrazide into the hyaluronic acid solution, stir well; Weigh carbodiimide hydrochloride in a ratio of ~5.0” and add it to hyaluronic acid solution, stir for 10min~20min; then adjust the pH value to 6~8 with sodium hydroxide solution to obtain hyaluronic acid hydrogel; The glue was washed 2 to 3 times in ultrasonic wave with deionized water, and then put into a lyophilizer for lyophilization after being frozen.

Embodiment 2

[0015] Example 2 Preparation of antibody against axonal growth inhibitory factor receptor NgR

[0016] The full-length amino acid sequence of rat NgR was found in the Protein database, and sequence analysis showed that several regions with high antigenicity served as immunogenic regions. According to the antigenicity, specificity and hydrophilicity of the sequence, the peptide segment DNNLQALPDNTFRD with amino acid residues 163-176 was selected as the antigenic fragment; the DNNLQALPDNTFRD sequence was synthesized by solid-phase synthesis, purified by high performance liquid chromatography, and completely Freund's adjuvant Cross-linking, a small amount of multiple immunization of rabbits. After 6 weeks of immunization, blood was collected from the carotid artery, the serum was precipitated naturally at 4°C, and centrifuged at 4000r / min for 20min to obtain the antibody serum of the axonal growth inhibitory factor receptor NgR.

Embodiment 3

[0017] Example 3 Oxidative Grafting of NgR Antibody

[0018] Add sodium periodate into the antibody phosphate buffer at a concentration of 10 mg / ml, stir gently for 30 minutes, dialyze in normal saline for 3 hours, and then filter the bacteria with a bacteria filter to obtain a sterile antibody solution; the cross-linked The hydrogel was sterilized three times in 75% alcohol, then washed three times with sterile phosphate buffer saline, mixed with sterile antibody, and reacted for 10 to 20 hours in an ultra-clean bench to obtain axon growth inhibitory factor A hyaluronic acid hydrogel of receptor antibodies, known as a cross-linked antibody-releasing system.

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PUM

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Abstract

The invention relates to a biological material used to recover hurt brain. Wherein, it uses transparent hyaluronic acid as raw material, guided by carbodiimide hydrochlorate (EDC), to use adipic acid adipoyl (ADH) to cross, to obtain the hyaluronic acid gel, as the carrier of graft antibody; and uses antibody solidification technique to graft the antibodies of axon growth restrain factor MAG, OMgp and Nogo-A to the hyaluronic acid gel, to be the antibody transfer system carrying special antibody and sensitive to pH value. The invention can be used to seal the never axon regeneration restrain factor of myelin and accelerate the regeneration of hurt brain organism.

Description

technical field [0001] The invention relates to a biological material for repairing brain damage and a preparation method thereof. Background technique [0002] Regenerative repair after brain injury is a difficult point in contemporary medicine. Studies have found that one of the reasons for the difficulty in brain nerve regeneration is that axonal degeneration and myelin sheath disintegration occur after neuron injury, and Nogo protein (Nogo-A), myelin-associated glycoprotein (MAG) and Substances such as oligodendrocyte myelin glycoprotein (OMgp) can inhibit the growth of nerve axons, and are called axon growth inhibitory factors. These inhibiting axon growth factors combine with their receptor NgR in vivo to inhibit the growth of nerve axons. Medical research has also found that blocking the combination of Nogo-A, MAG and OMgp with NgR with specific antibodies can eliminate the inhibitory effect of inhibiting axon growth factors on nerve regeneration and increase the pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/28A61L27/54
Inventor 徐群渊刘丙方候少平崔福斋田维明马军张进禄杨慧高尔静姬曼
Owner 首都医科大学北京神经科学研究所
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