Biological material for brain injury renovation and its preparing process
A biomaterial, post-brain injury technology, applied in medical science, prostheses, coatings, etc., can solve the general tumor risk, cannot effectively control the release of antibodies and other problems, achieve good cell compatibility, and promote functional recovery. , the effect of good compatibility
Examples
Embodiment 1
[0013] Example 1 Preparation of Hyaluronic Acid Hydrogel
[0014] Prepare a hyaluronic acid solution with a mass percentage of 1% with deionized water, add 1M hydrochloric acid solution dropwise to the hyaluronic acid solution, and stir at the same time to fully mix the two until the pH value reaches 4.75; Hydrazide: hyaluronic acid = 1: 4 ~ 10" ratio Weigh adipate dihydrazide into the hyaluronic acid solution, stir well; Weigh carbodiimide hydrochloride in a ratio of ~5.0” and add it to hyaluronic acid solution, stir for 10min~20min; then adjust the pH value to 6~8 with sodium hydroxide solution to obtain hyaluronic acid hydrogel; The glue was washed 2 to 3 times in ultrasonic wave with deionized water, and then put into a lyophilizer for lyophilization after being frozen.
Embodiment 2
[0015] Example 2 Preparation of antibody against axonal growth inhibitory factor receptor NgR
[0016] The full-length amino acid sequence of rat NgR was found in the Protein database, and sequence analysis showed that several regions with high antigenicity served as immunogenic regions. According to the antigenicity, specificity and hydrophilicity of the sequence, the peptide segment DNNLQALPDNTFRD with amino acid residues 163-176 was selected as the antigenic fragment; the DNNLQALPDNTFRD sequence was synthesized by solid-phase synthesis, purified by high performance liquid chromatography, and completely Freund's adjuvant Cross-linking, a small amount of multiple immunization of rabbits. After 6 weeks of immunization, blood was collected from the carotid artery, the serum was precipitated naturally at 4°C, and centrifuged at 4000r / min for 20min to obtain the antibody serum of the axonal growth inhibitory factor receptor NgR.
Embodiment 3
[0017] Example 3 Oxidative Grafting of NgR Antibody
[0018] Add sodium periodate into the antibody phosphate buffer at a concentration of 10 mg / ml, stir gently for 30 minutes, dialyze in normal saline for 3 hours, and then filter the bacteria with a bacteria filter to obtain a sterile antibody solution; the cross-linked The hydrogel was sterilized three times in 75% alcohol, then washed three times with sterile phosphate buffer saline, mixed with sterile antibody, and reacted for 10 to 20 hours in an ultra-clean bench to obtain axon growth inhibitory factor A hyaluronic acid hydrogel of receptor antibodies, known as a cross-linked antibody-releasing system.
PUM
Login to View More Abstract
Description
Claims
Application Information
- IPC
- A61L27/28; A61L27/54
- Inventors
- 徐群渊; 刘丙方