CHO cell
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- F HOFFMANN LA ROCHE & CO AG
- Publication Date
- 2010-03-31
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
[0001] The present invention provides CHO cells having constitutively active mitogenic receptors. The cells are used for protein expression. The present invention thus belongs to the field of mammalian cell engineering and protein expression. Background of the invention
[0002] Expression systems for the production of recombinant polypeptides are well known in the art and are described, for example, in Marino, M.H., Biopharm. 2 (1989) 18-33; Goeddel, D.V., et al., Methods Enzymol. 185 (1990) 3-7; Wurm, F., and Bernard, A., Curr. Opin. Biotechnol. 10 (1999) 156-159. Such expression systems comprise host cells and suitable expression plasmids.
[0003] The basic elements of an expression plasmid are, for example, the prokaryotic plasmid propagation unit of Escherichia coli (E.coli), which comprises an origin of replication and a selectable marker, a eukaryotic selectable marker and one or more expression cassettes for expressing a structural gene of interest, each The expres...