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31 results about "Proliferative response" patented technology

Supression of allergic reactions by transdermal administration of allergens conjugated to cholera toxin or fragments thereof

The present invention discloses the use of the non-toxic cell-binding B subunit of CT (CTB), and holotoxin CT that is devoid of ADP-ribosylating activity, as adjuvants for enhancing transcutaneous immune response to a co-administered protein allergen. It was found that topical administration of CTB to mice induced serum antibody response against itself comparable to those evoked by CT, but was inefficient at promoting systemic antibody responses against an admixed prototype protein allergen. To the contrary co-administration of either CT or CTB with allergen led to vigorous antigen-specific T cell proliferative responses in lymph nodes draining the cutaneous site of administration and at distant systemic sites. Consistent with these observations, it was found that CTB selectively potentiated Th1-driven responses without affecting Th2-dependent responses. Cutaneously applied CT enhanced serum IgE responses to a co-administered allergen, while CTB partially suppressed epicutaneously induced IgE responses to the same allergen.
Owner:DUOTOL

Cell systems and methods for detecting proliferation acitvity

The invention provides proliferative response indicator cell having a vertebrate cell having a luciferase encoding nucleic acid and a heterologous proliferation factor receptor encoding nucleic acid, wherein each of the encoding nucleic acids are operationally linked to expression elements for co-expression of a luciferase polypeptide and a heterologous proliferation factor receptor. The invention also provides a method of determining a cell proliferative response to a proliferation factor. The method includes: (a) contacting a vertebrate cell expressing luciferase and a proliferation factor receptor with a proliferation factor for sufficient time for the proliferation factor to bind to the proliferation factor receptor; (b) culturing the contacted cell expressing luciferase for at least one generation, and (c) measuring the amount of light emission, wherein the luciferase expression is driven from a promoter non-responsive to the proliferation factor and the light emission directly correlates with proliferation factor-mediated cell proliferation. The proliferation factor can be a growth factor, a cytokine or a hormone or an agonist or antagonist thereof. The methods of the invention additionally include determining the effect of an inhibitor of the proliferation factor. Cells used in the method are contacted with a proliferation factor in the presence of a sample suspected of containing an inhibitor of the proliferation factor. The inhibitor can be neutralizing antibody, or binding fragment thereof, to the proliferation factor. The methods of the invention also are applicable as an indicator of cell health or viability. The invention further provides a diagnostic system. The diagnostic system includes a plurality of different vertebrate cell lines each encoding a luciferase gene and a different proliferation factor receptor, the luciferase gene being operationally linked to a promoter non-responsive to a proliferation factor bound by the proliferation factor receptor, wherein light emission from each of the different cell lines being characterized as directly correlating with proliferation factor-mediated cell proliferation.
Owner:AMGEN INC

Saturated fatty chain alcohol His-Gly-AA tripeptide ester, synthetic method and application thereof

InactiveCN101899090AExcellent immunosuppressive effectTripeptide ingredientsPeptide preparation methodsChemistrySplenic lymphocyte
The invention relates to 12 saturated fatty chain alcohol His-Gly-AA tripeptide ester conjugates with immunosuppressive activity in a general formula His-Gly-AA-O-CH2-(CH2)nCH3 I, wherein AA stands for Glu or Lys. In saturated fatty chain alcohol, n is equal to 6, 8, 10, 12, 14 or 16. the invention also relates to a preparation method and application of the conjugates as an immunosuppressant. An experimental result of the saturated fatty chain alcohol His-Gly-AA tripeptide ester which has the inhibition effects of splenic lymphocyte mitogen breeder reaction and macrophage phagocytosis activity indicates that the conjugates of the invention have excellent immunosuppressive action and can be clinically used as an immunosuppressant.
Owner:CAPITAL UNIVERSITY OF MEDICAL SCIENCES

Conjugates of saturated aliphatic chain alcohol, dexamethasone, and Glu-Asp-Gly, preparation, nano structure, and applications thereof

InactiveCN104211760AExcellent immunosuppressive effectAntipyreticAnalgesicsNano structuringSide effect
The invention relates to conjugates of saturated aliphatic chain alcohol, dexamethasone, and Glu-Asp-Gly, preparation, a nano structure, and applications thereof. The invention discloses 6 saturated aliphatic chain alcohol modified dexamethasone-Glu-Asp-Gly conjugates represented by the formula 10 a-f, wherein in the formula the n represents 7, 9, 11, 13, 15, or 17. The invention also discloses a preparation method, a nano structure, immunity inhibition activity, inflammation inhibition activity, and pain relieving activity of the conjugates. The invention also finds that the conjugates do not have any side effect leading to osteoporosis. The researches on the inhibition effect of the conjugates on splenic lymphocyte mitogen proliferation reactions and the survival time after mouse retroauricular cardiac muscle transplant show that the conjugates have an excellent immunity inhibition effect. The researches on the effect of the conjugates on the swelling degree of mouse ears which are inflamed due to xylene show that the conjugates have an excellent anti-inflammation effect. The researches on the effect of the conjugates on mouse heat radiation tail flick time show that the conjugates have an excellent pain-relieving effect. The researches on the effect of the conjugates on the mouse thigh bones show that the conjugates cannot cause osteoporosis. So the conjugates have a wide application prospect in the preparation of immunity inhibition drugs.
Owner:CAPITAL UNIVERSITY OF MEDICAL SCIENCES

Antigen-antibody complex for preventing and/or treating avian influenza

The invention provides an antigen-antibody complex for preventing and/or treating avian influenza, which comprises inactivation avian influenza totiviruses which are used as antigen and an immune body thereof, and the mass concentration ratio of the antigen and the immune body is more than 1. After entering organisms to perform initial immunization, the antigen-antibody complex stimulates the organisms again to induce immunoreaction, and immune response is quick in speed and strong in reaction; the antigen-antibody complex used as a carrier is more favorable for capturing and presenting antigen presenting cells and can also strengthen a breeder reaction of T cells effectively; purified totiviruses used as the antigen increase the molecular weight of the complex, are more favorable for ingestion of immunocyte of the organisms on the antigen, cause the more extensive immunoreaction quickly, and have a significance for preventing avian influenza viruses of which the antigen is easy for variation. A preparation of the antigen-antibody complex does not need to use solid carriers, does not cause intense stimulus on the organisms or initiates the organisms to generate an adverse reaction, and is simple to prepare and safe and convenient to use.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI

Conjugates of saturated aliphatic chain alcohol, dexamethasone, and His-Gly-Glu, preparation, nano structure, and applications thereof

InactiveCN104211761AExcellent immunosuppressive effectAntipyreticAnalgesicsOsteoporosisSide effect
The invention relates to conjugates of saturated aliphatic chain alcohol, dexamethasone, and His-Gly-Glu, preparation, a nano structure, and applications thereof. The invention discloses 6 saturated aliphatic chain alcohol modified dexamethasone-His-Gly-Glu conjugates represented by the formula 10 a-f, wherein in the formula the n represents 7, 9, 11, 13, 15, or 17. The invention also discloses a preparation method, a nano structure, immunity inhibition activity, inflammation inhibition activity, and pain relieving activity of the conjugates. The invention also finds that the conjugates do not have any side effect leading to osteoporosis. The researches on the inhibition effect of the conjugates on splenic lymphocyte mitogen proliferation reactions and the survival time after mouse retroauricular cardiac muscle transplant show that the conjugates have an excellent immunity inhibition effect. The researches on the effect of the conjugates on the swelling degree of mouse ears which are inflamed due to xylene show that the conjugates have an excellent anti-inflammation effect. The researches on the effect of the conjugates on mouse heat radiation tail flick time show that the conjugates have an excellent pain-relieving effect. The researches on the effect of the conjugates on the mouse thigh bones show that the conjugates cannot cause osteoporosis. So the conjugates have a wide application prospect in the preparation of immunity inhibition drugs.
Owner:CAPITAL UNIVERSITY OF MEDICAL SCIENCES

Nitrogenatd trans-stilbene analogs, method for the obtention and medical applications thereof

This invention is related to new nitrogenated trans-stilbene analog compounds, more specifically, imine, pyrrol and Indole derivatives, with procedures for the preparation and use thereof as pharmaceutical compositions for the treatment and / or chemoprevention of those mammalian diseases such as cancer, fibrosclerosis and acute / chronic inflammation, graft-versus-host reaction, ischemic-reperfusion tissue injury in stroke and heart attack, neurodegeneration, and during organ transplantation, whose pathogenic and pathophysiological mechanisms depend on or are significantly contributed by undesirable oxidative stress, angiogenic and proliferative responses.
Owner:DOMINION PHARMAKINE +1

DC cell preparation method for improving antigen presenting T cell and improving T cell killing efficiency and application thereof

The invention provides a DC cell preparation method for improving antigen presenting T cells and improving T cell killing efficiency and application thereof. A tumor antigen modified by 4-phenyl isothiocyanate alpha-D-mannopyranoside glycosylation is loaded on a DC cell, SDF-1 and IL-8 chemotactic factors are added, the DC cell is chemotactic, more mature DCs are obtained, interaction between the DC and T cell is enhanced, T cell activation is further promoted, a gene expression silencing agent shRNA is introduced into the DC cell, an IDO gene is silenced, the surface antigen of the DC is obviously increased, and the proliferation reaction of T lymphocytes is enhanced and stimulated; after the DC cells and the T cells are subjected to mixed culture, the pH value is adjusted, and the DC cells and the T cells are cultured in a weak acid environment, so that the antigen presentation efficiency of the DC cells and the T cells is improved. The result shows that the antigen presentation efficiency between the DC cells and the T cells is higher, and the T cells with higher antigen specificity and higher killing efficiency can be obtained through stimulation.
Owner:北京荟科柘生物科技有限公司

Method for detecting lymphopoiesis by utilizing medicament cytochalasin B

InactiveCN104017876AMorphological observation is intuitive and objectiveReduce experiment costMicrobiological testing/measurementMaterial analysisBiophysicsBinucleated cells
The invention belongs to the technical field of biological detection, and particularly relate to a method for detecting lymphopoiesis by utilizing a medicament cytochalasin B. The principle of the invention is that the medicament cytochalasin B can retard cell division and cannot retard nuclear division, so that cells which are subjected to breeder reaction are treated by the cytochalasin B to form visual binucleated cells; peripheral blood lymphocyte can enable corresponding lymphocyte to be cloned and multiplicated under the stimulation of phytohemagglutinin, and ionizing radiation can inhibit multiplication of lymphocyte; human peripheral blood can be irradiated by a 2Gygamma ray, and the cytochalasin B is added to treat after the stimulation by PHA (phytohemagglutinin), and difference of binuclear rate of radiated and non-radiated lymphocyte is detected; the lymphocyte stimulated by PHA forms binucleated cells, and the binuclear rate of radiated lymphocyte decreases obviously, which shows that the multiplication level of lymphocyte can be reflected well by detecting the binuclear rate of lymphocyte by utilizing the cytochalasin B. The method provided by the invention is visual in detecting indexes, is simple, rapid and economical in operation, and is accurate in result.
Owner:FUDAN UNIV
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