A kind of porcine circovirus type 2 immune protection polypeptide and vaccine
A porcine circovirus, immune protection technology, applied in the direction of viral peptides, viruses, antiviral agents, etc., can solve the problem of no relevant immune protection
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Embodiment 1
[0024] Embodiment 1: Polypeptide and vaccine preparation
[0025] Main Reagents and Materials
[0026] Porcine circovirus type 2 (PCV2) SH strain was isolated, identified and preserved in our laboratory; ISA50V adjuvant was purchased from SEPPIC, France; goat anti-mouse IgG-HRP, SPA-HRP and DAB chromogenic kits were purchased from Wuhan Boster Biotech Engineering company; other routinely used reagents are domestic or imported analytically pure.
[0027] Peptide Sequence and Synthesis
[0028] According to the amino acid sequence of PCV2 Cap protein epitope and T cell epitope, the following 4 polypeptides were designed and synthesized by Shanghai Moxi Biotechnology Co., Ltd. with a purity of more than 90%.
[0029] : as shown in sequence 1 in the sequence table,
[0030] P5013: as shown in sequence 2 in the sequence listing,
[0031] P2914: as shown in sequence 3 in the sequence listing,
[0032] P1300: as shown in sequence 4 in the sequence listing.
[0033] 1.3 Vaccine ...
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