A kind of porcine circovirus type 2 immune protection polypeptide and vaccine

A porcine circovirus, immune protection technology, applied in the direction of viral peptides, viruses, antiviral agents, etc., can solve the problem of no relevant immune protection

CN104098657BActive Publication Date: 2017-06-27NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2017-06-27

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Abstract

The invention relates to the technical field of porcine circovirus, in particular to a porcine circovirus 2 (PCV2) immune protection polypeptide, and a vaccine containing the PCV2 immune protection polypeptide. The amino acid sequence of the PCV2 immune protection polypeptide is shown in the sequence table 1-4. According to the vaccine, tests show that after 42-63 days of first immune of a vaccine of P5013 and a vaccine of P1300, the antibody titer and the neutralizing antibody titer of an ELISA antibody are remarkably increased, the lymphocyte proliferative response and IL-4 and IFN-Gamma cytokines are significantly higher than those of a control group, and the antibody level and the cellular immunity level of the vaccine group of P5013 are the highest; mice tests show that PCV2 immune response induced by the synthetic peptide vaccine of P5013 is the highest and the strongest; pig tests find that the synthetic polypeptide vaccine of P5013 can induce pigs to generate a PCV2-specific immune response, can reduce viremia caused by PCV2 virulent virus attack, alleviate the clinical symptoms and pathological lesions of viremia, and has immune protection effect on PCV2.
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Description

technical field

[0001] The invention relates to the technical field of porcine circovirus, in particular to a porcine circovirus type 2 immune protection polypeptide and a vaccine prepared by using the porcine circovirus type 2 immune protection polypeptide. Background technique

[0002] Porcine circovirus type 2 (Porcine circovirus 2, PCV2) is a member of the Circovirus genus of the family Circoviridae, which can cause a variety of diseases such as multisystemic wasting syndrome (PMWS) and porcine dermatitis nephropathy syndrome (PDNS) in weaned piglets. It has brought huge economic losses to the global pig industry. Cap protein is a type-specific antigen of PCV2, which has good immunogenicity, can effectively produce neutralizing antibodies through immunization, neutralize virus infection, and effectively induce protective immune responses against virus attack. At present, commercialized vaccines include inactivated PCV2 vaccine and inactivated PCV2 baculovirus vaccine, b...

Examples

Embodiment 1

[0024] Embodiment 1: Polypeptide and vaccine preparation

[0025] Main Reagents and Materials

[0026] Porcine circovirus type 2 (PCV2) SH strain was isolated, identified and preserved in our laboratory; ISA50V adjuvant was purchased from SEPPIC, France; goat anti-mouse IgG-HRP, SPA-HRP and DAB chromogenic kits were purchased from Wuhan Boster Biotech Engineering company; other routinely used reagents are domestic or imported analytically pure.

[0027] Peptide Sequence and Synthesis

[0028] According to the amino acid sequence of PCV2 Cap protein epitope and T cell epitope, the following 4 polypeptides were designed and synthesized by Shanghai Moxi Biotechnology Co., Ltd. with a purity of more than 90%.

[0029] : as shown in sequence 1 in the sequence table,

[0030] P5013: as shown in sequence 2 in the sequence listing,

[0031] P2914: as shown in sequence 3 in the sequence listing,

[0032] P1300: as shown in sequence 4 in the sequence listing.

[0033] 1.3 Vaccine ...