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Panax ginseng glycopeptide as well as preparation method and medical application thereof

A technology of ginseng and glycopeptide, applied in the field of medicine, can solve the problems of low extraction rate, etc., and achieve the effect of significant curative effect, enhancement of cellular immune function, and improvement of immune function.

Active Publication Date: 2021-07-23
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above preparation methods mainly adopt low-concentration ethanol extraction, high-concentration ethanol precipitation separation, filtration and activated carbon purification, and the extraction rate is low, about 0.45% to 7%.

Method used

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  • Panax ginseng glycopeptide as well as preparation method and medical application thereof
  • Panax ginseng glycopeptide as well as preparation method and medical application thereof
  • Panax ginseng glycopeptide as well as preparation method and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Extraction: Take dried raw sun-dried ginseng, crush it and pass it through a 20-mesh sieve, add 14 times the amount of distilled water, decoct and extract twice at 100°C, for 3 hours each time, filter, combine the filtrates, concentrate until the specific gravity of the solution is 1.04, and obtain ginseng water extract concentrate;

[0027] Separation: Add high-concentration ethanol to the ginseng water extract concentrate, stir while adding, stop stirring after the alcohol concentration of the solution reaches 70%, let it stand for 48 hours, filter, and obtain two parts of filtrate and precipitate, the filtrate part, decompress Recover the ethanol solution to obtain the crude extract of ginseng total saponins; the precipitated part is the crude extract of ginseng glycoprotein;

[0028] Enzymolysis: take the crude extract of ginseng glycoprotein and add distilled water to make the substrate concentration 10%, add flavor protease and papain at a ratio of 1:1 to make the...

Embodiment 2

[0032] Extraction: Take dried raw sun-dried ginseng, crush it and pass it through a 20-mesh sieve, add 16 times the amount of distilled water, decoct and extract it at 100°C for 3 times, each time for 1 hour, filter, combine the filtrates, concentrate until the specific gravity of the solution is 1.06, and obtain ginseng water extract concentrate;

[0033] Separation: Add high-concentration ethanol to the ginseng water extract concentrate, stir while adding, stop stirring after the alcohol concentration of the solution reaches 75%, let it stand for 24 hours, filter, and obtain two parts of filtrate and precipitate, the filtrate part, decompress Recover the ethanol solution to obtain the crude extract of ginseng total saponins; the precipitated part is the crude extract of ginseng glycoprotein;

[0034] Enzymolysis: take the crude extract of ginseng glycoprotein and add distilled water to make the substrate concentration 13%, add flavor protease and papain at a ratio of 1:1 to ...

Embodiment 3

[0038] Extraction: Take dried raw sun-dried ginseng, crush it and pass it through a 20-mesh sieve, add 20 times the amount of distilled water, decoct and extract it at 100°C for 4 times, each time for 2 hours, filter, combine the filtrates, concentrate until the specific gravity of the solution is 1.05, and obtain ginseng water extract concentrate;

[0039] Separation: Add high-concentration ethanol to the ginseng water extract concentrate, stir while adding, stop stirring after the alcohol concentration of the solution reaches 72%, let it stand for 36 hours, filter, and obtain the filtrate and the precipitate, and the filtrate part, decompress Recover the ethanol solution to obtain the crude extract of ginseng total saponins; the precipitated part is the crude extract of ginseng glycoprotein;

[0040] Enzymolysis: take the crude extract of ginseng glycoprotein and add distilled water to make the substrate concentration 15%, add flavor protease and papain at a ratio of 1:1 to ...

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Abstract

The invention relates to panax ginseng glycopeptide as well as a preparation method and medical application thereof, and belongs to the field of medicines. Panax ginseng is used as a raw material, and panax ginseng glycopeptide is obtained through the steps of extraction through a decoction method, separation through an ethanol precipitation method, hydrolysis through a double-enzyme enzymolysis method, dialysis, freeze drying and the like, wherein the yield of the panax ginseng glycopeptide is 14% or above, and the purity is 90% or above. Animal model experiments are carried out, and results show that the panax ginseng glycopeptide can enhance the cellular immune function, humoral immune function, mononuclear-macrophage phagocytosis function and NK cell activity of immunocompromised mice. The panax ginseng glycopeptide is novel in preparation method, relatively high in yield and purity and suitable for industrial production, serves as an active pharmaceutical ingredient, is expected to be developed into novel drugs for preventing and treating low-immunity diseases and auxiliary drugs for low-immunity diseases caused by tumors, cancers and the like, and has a wide application prospect. Clinical guidance and medication basis are provided for prevention and treatment of hypoimmunity diseases, so that the pain of patients is relieved, and the living quality of the patients is improved.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a ginseng glycopeptide, a preparation method and a medical application. Background technique [0002] Ginseng is the dried root and rhizome of Panax ginseng C.A.Mey., a plant of the family Araliaceae. It has the effects of invigorating vital energy, restoring pulse and solidifying, nourishing spleen and lung, promoting body fluid and nourishing blood, calming the nerves and improving intelligence. The material basis for ginseng to exert multiple pharmacological effects mainly includes components such as saponins, polysaccharides, polypeptides, glycopeptides, proteins, and volatile oils. In recent years, peptide components have attracted extensive attention from scholars at home and abroad for their unique biological activity and high bioavailability. With the improvement of extraction and separation technology and analysis technology, ginseng glycopeptide components with bette...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P37/04
CPCC12P21/06C07K1/34A61K38/011A61P37/04Y02P20/133Y02P20/59
Inventor 陈长宝张庆贺俞萍张涛李琼陈雪战宇杜连云陈雨秋谢昊臻
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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