Application of cordyceps sinensis mycelium solid fermentation water extract in regulation of immune function

A technology of Cordyceps sinensis and solid fermentation, which is applied in the directions of application, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems that products with immunomodulatory effects have not yet been seen.

Inactive Publication Date: 2013-07-10
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are a variety of Cordyceps health products with immune regulation function on the market, such as Cordyceps Shenbao, Tianshi Cordyceps Mycelium Capsules, etc., but m

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019]The collected fruiting bodies of Cordyceps sinensis (Berk.) Sacc. were separated from the tissues by conventional methods, and were inoculated on the slant medium of Cordyceps sinensis (according to 100% of its total mass fraction, It consists of 1% glucose, 0.5% peptone, 0.3% malt extract powder, 0.3% yeast extract, 2% agar and the rest of water) to obtain pure strains. Entity, tissue separation under aseptic conditions, inoculation to Cordyceps sinensis slant medium, placed at 15 ° C, shading culture for 5 ~ 7d. Taking the starting strain as a control, comparing the growth ability of the obtained strain, mainly observing the growth of the mycelia growth rate, color change speed, and the number of primordia formed. Finally, test the strains with better growth on the slope.) Rejuvenate the strains.

[0020] Insert the original strain into sterile Cordyceps sinensis slant medium according to the size of 0.3cm×0.5cm (based on its total mass fraction of 100%, consisting of ...

Embodiment 2

[0029] The collected fruiting bodies of Cordyceps sinensis (Berk.) Sacc. were separated from the tissues by conventional methods, and were inoculated on the slant medium of Cordyceps sinensis (according to 100% of its total mass fraction, Consists of 1.5% glucose, 0.8% peptone, 0.4% malt extract powder, 0.4% yeast extract, 2% agar and the rest of the water) to obtain pure strains, in order to prevent the degradation of the strains, according to the conventional method of Example 1 Rejuvenate the bacteria.

[0030] Insert the original strain into the sterile Cordyceps slant culture medium (according to its total mass fraction of 100%, consisting of 1.5% glucose, 0.8% peptone, 0.4% malt extract powder, and 0.4% yeast extract) according to the size of 0.3cm×0.5cm. %, agar 2% and the rest of water), at 13°C, after 33 days of dark cultivation, and 5 days of light, the strains with fast color change and neat mycelium growth were selected as the mother species.

[0031] Take 15g of ...

Embodiment 3

[0039] The collected fruiting bodies of Cordyceps sinensis (Berk.) Sacc. were separated from the tissues by conventional methods, and were inoculated on the slant medium of Cordyceps sinensis (according to 100% of its total mass fraction, It consists of 2% glucose, 1% peptone, 0.5% malt extract powder, 0.5% yeast extract, 2% agar and the rest of the water) to obtain pure strains. Bacteria are rejuvenated.

[0040] Insert the original strain into sterile Cordyceps sinensis slant medium according to the size of 0.3cm×0.5cm (based on its total mass fraction of 100%, consisting of 2% glucose, 1% peptone, 0.5% malt extract powder, 0.5% yeast extract %, agar 2% and the rest of water), at 15°C, after dark culture for 35 days and light for 6 days, select the strain with fast color change and neat mycelium growth as the mother species.

[0041] Take 20g of glucose, 10g of peptone, 5g of malt extract powder, and 5g of yeast extract, dissolve them in 960g of sterile water, adjust the pH...

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Abstract

The present invention relates to application of cordyceps sinensis mycelium solid fermentation water extract in preparation of products with immune regulation functions. The cordyceps sinensis mycelium solid fermentation water extract of the invention is prepared by using wild cordyceps sinensis (Berk.) sporocarp through the steps of strain collection, preservation and rejuvenation, preparation of parent strain, first-grade liquid strain and second-grade liquid strain, preparation of solid fermentation objects, and water extraction. The obtained cordyceps sinensis mycelium solid fermentation water extract is used for conducting immune regulation experiments for mice. Experiment results show that the cordyceps sinensis mycelium solid-state fermentation water extract can substantially enhance macrophage phagocytosis of mice with low immune function, can adjust the phagocytosis rate of the mice with low immune function to a level approaching normal, has immune regulation function and can be used for preparing products with immune regulation functions.

Description

technical field [0001] The invention relates to the application of a fermented extract of Cordyceps sinensis mycelium, in particular to the application of a solid fermented water extract of Cordyceps sinensis (Berk.) Sacc.) mycelium in regulating immune function. Background technique [0002] In recent years, the research and development of natural medicines with immunomodulatory effects has become a hot spot at home and abroad. As a nourishing Chinese medicine, Cordyceps has the effects of protecting the lungs and kidneys, relieving cough and reducing phlegm, treating impotence and nocturnal emission, and sore waist and knees. As the most important type of fungus in Cordyceps sinensis, Cordyceps sinensis has the functions of nourishing kidney and lung, regulating neuroendocrine, strengthening metabolism, regulating human immunity, etc. and has extremely high commercial value, so it has become one of the most valuable types of biological drugs. In the study on the immune re...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P37/02A61P37/04A01G1/04
Inventor 闫文娟李泰辉
Owner GUANGDONG INST OF MICROORGANISM
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