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System for characterizing macrophage phagocytosis microorganisms of pompanos

A macrophage and microorganism technology, applied in the field of cell biology, can solve the problems of isolation, purification, culture and characterization of macrophages in the kidney of oval pomfret head kidney, etc.

Active Publication Date: 2017-05-31
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] There are few reports on the immune function of S. ovata. The head kidney is an important immune organ of fish. There are no reports on the isolation, purification, culture and characterization of macrophages from the head kidney of S. ovata.

Method used

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  • System for characterizing macrophage phagocytosis microorganisms of pompanos
  • System for characterizing macrophage phagocytosis microorganisms of pompanos
  • System for characterizing macrophage phagocytosis microorganisms of pompanos

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Embodiment Construction

[0100] In order to better explain the technical solutions of the present invention, various embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

[0101] 1. Experimental materials

[0102] 1.1 Experimental fish

[0103] Oval pomfret, commonly known as golden pomfret. The body is pomfret-shaped, high and flat on the sides; the body length is 1.7-1.9 times the body height and 3.8 times the head length. The tail peduncle is short and thin, and the side is flat. Head small, taller than long. The occipital crest is prominent. The he...

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Abstract

The invention discloses a system for characterizing macrophage phagocytosis microorganisms of pompanos. The pompanos are dissected and separated through a surgical knife and tweezers to obtain heads and kidneys; the heads and the kidneys are abraded through a cell sieve to obtain cell suspension containing macrophages, and the macrophages of the heads and the kidneys are obtained through a Percoll solution density gradient centrifugal tube. The survival amount of cells cultured in vitro through a trypan blue dyeing manner is counted, and the survival condition in one week is detected. The system is used for carrying out a phagocytosis experiment, and phagocytosis conditions of nocardia, yeast and the like of the pompanos by the head and kidney macrophages of trachinotus ovatus are determined, and a phagocytosis function is observed. An experiment result of the system shows that the macrophages of the pompanos can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the pompanos can be observed very well. The system disclosed by the invention lays a foundation for further researches on physiology, pathology and toxicology of the macrophages of the pompanos.

Description

technical field [0001] The invention belongs to the field of cell biology, and relates to a system for characterizing the interaction between immune cells and microorganisms, in particular to a system for characterizing the microorganisms phagocytized by pomfret macrophages. Background technique [0002] Oval pomfret (Trachinotus ovatus) (Linnaeus), commonly known as golden pomfret, yellow wax pomfret, belongs to Osteichthyes, Vertebrate Phylum, Osteichthyes, Perciformes, Trevalidae, Pomfrets genus. Oval pomfret has a flat side, oval shape, large body, fast growth, and strong disease resistance. In natural waters, pompano pomfret prefers pomfret pomfret to forage and migrate in clusters, vying for food fiercely, mainly feeding on small molluscs and small snails. The large individual weighs 5-10kg, and the meat is tender and delicious. It is a valuable edible fish. [0003] Pomfret ovata is a seawater fish with strong disease resistance, and there were few reports on its di...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N15/10G01N1/28
CPCG01N1/2813G01N15/10G01N33/5055G01N2015/1006
Inventor 鲁义善夏立群蔡佳简纪常
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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