Separation and culture method for different cellular components of human mammary tissue

Abstract

The invention relates to a separation and culture method for different cellular components of a human mammary tissue, and belongs to the technical field of cell culture. The method comprises the following steps: (1) sampling a fresh human mammary tissue specimen; (2) carrying out mechanical shearing and collagenase and hyaluronidase combined digestion to prepare a mammary tissue cell suspension; (3) carrying out low-speed and differential centrifugation to layer and separate the different cellular components; (4) carrying out centrifugation, washing and adherent culture for multiple times to purify the cell components, so as to obtain the different cellular components of the mammary tissue, which comprise epithelial cells, matrix cells and preadipocytes. The separation and culture method disclosed by the invention can be used for quickly, simply and effectively separating and purifying the different cellular components from the same mammary tissue; the cultured mammary gland cells are sufficient in quantity, good in cell viability and purity which is up to 95% above; the separation and culture method can provide very useful materials for molecular biology study associated with mammary glands and breast cancer, and establishes a foundation for construction of a mammary gland microenvironment multi-cell culture model.

Description

technical field [0001] The invention relates to a method for separating and culturing tissue cells, in particular to a method for separating and culturing different cell components of human breast tissue, and belongs to the field of cell biology and biotechnology. Background technique [0002] Breast cancer is a malignant tumor that usually occurs in breast epithelial tissue. It is one of the most common tumors in women, seriously affecting women's physical and mental health and even threatening their lives. According to WHO statistics, the incidence rate accounts for 7-10% of various malignant tumors in the whole body. About 1.2 million women in the world suffer from breast cancer every year, and 500,000 people die from the disease. [0003] The occurrence and development of tumor is a complex process involving many factors. Under normal conditions, the homeostasis within epithelial tissues is maintained by dynamic interactions between epithelial cells and their microenvir...

AI Technical Summary

Problems solved by technology

However, most of the cell models used in breast cancer research in the past are only for breast cancer epithelial cells, and there are few studies on the influence of other cell components in other breast tissues on the occurrence and development of breast cancer. One of the reasons is that the current isolation of breast tissue cells Most of the methods and cultures are carried out for a single cell type, and no other cell components in the same breast tissue have been isolated and cultured, which greatly limits the construction and research of multilayer cell models of breast cancer cell microenvironment

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