The embodiment of the invention discloses a radio frequency scalpel for surgery. The scalpel comprises a tool assembly, a handle assembly, a water injection assembly, a biopsy assembly and an illumination assembly. The handle assembly comprises a body and a handle; the tool assembly is arranged at the front end of the body of the handle assembly, the water injection assembly is arranged at the upper end of the body of the handle assembly, the biopsy assembly is arranged at the rear end of the body of the handle assembly, and the illumination assembly is arranged at the front end of the handleof the handle assembly. The scalpel can be applied to a general surgery department, a thoracic surgery department, a hepatological surgery department, a neurosurgery department, a head and neck surgery department, a bone surgery department, an ear-nose-throat surgery department, a urinary surgery department, an anorectal surgery department, a breast surgery department, a pediatric surgery department, an abdomen surgery department, a pancreas surgery department, a digestion surgery department, a vascular surgery department, an endocrine surgery department, a plastic surgery department, an oncology department, a beautifying department, a burns department, a clinical lab, a pediatric department, a gynecology department and other departments. Tissue suction, biopsy sampling, illumination, normal saline injection, low-frequency direct current storage and other functions in surgery can be achieved.