The invention discloses a construction method of a mouse
autism intervention model, and belongs to the technical field of neurobiology and
animal model construction. Comprising the following steps: S1, selecting a pregnant C57BL / 6J female mouse, performing VPA induction on the 12th to 13th
embryo to form an experimental group, and injecting normal
saline into another mouse to form a control group; s2, performing AAV-shRNA targeted mGluR5
virus injection on the mice in the experimental group, and retaining the injection for 15 minutes after the injection so as to avoid
backflow; s3, recovering the postoperative mouse for 3 weeks, performing behavioral detection on the mouse at the age of 7 weeks, and performing
protein and
morphological analysis on the hippocampal tissue after an experiment; s4, carrying out behavioral experiments to evaluate ASD sample behavioral characteristics, detecting mGluR5 and PSD95
protein expression levels in the
hippocampus tissues by using
Western blot, and analyzing changes of synaptic structures by adopting Golgi
staining. The VPA model provided by the invention can be used for wider
autism research, and a model platform which is low in
toxicity, easy to operate and repeatable is provided.