Human liquid state derma preparation for injection and its preparation method

Abstract

The invention relates to a human body liquid state corium layer preparation for injection and a preparation method thereof, and belongs to the field of medical bio engineering. The preparation method of the human body liquid state corium layer preparation for injection comprises the following steps: digesting and separating the skin from a curer himself; obtaining corium fibroblast stem cell, a fibroblast fore body cell and a fibroblast cell; performing the extraneous omnirange blood serum-free cultivation and expansion of the cells; preparing effective component and normal saline injection or other injection collected as well as other suitable components selected arbitrarily. The effective component is composed of at least one of the corium f fibroblast stem cell, the fibroblast fore body cell and the fibroblast cell collected after being cultivated and collagen. In each-liter filling agent for injection, the total quantity of the effective cell component is 10,000,000 to 80,000,000, and the collagen contains 10-100 mg / ml filling agent for injection. The human body liquid state corium layer preparation has the advantages that the used material is derived from the curer himself, no rejection reaction and side effect occur, the effect is quick after remedy, the effect is obvious, the expression is natural, the effect action is durable and can be kept for a plurality of years.

Description

technical field

[0001] The invention relates to a human body liquid dermis preparation for injection and a preparation method thereof, more specifically a cellular tissue filler, which is used for filling and repairing human soft tissues, including removing wrinkles and repairing depressed scars. Its tissue filler contains autologous fibroblast stem cells, fibroblast precursor cells, fibroblasts and collagen after in vitro culture and expansion. Its preparation process uses serum-free culture materials, and finally prepares an injection suspension of tissue filler, which is injected into wrinkles, depressed scars, traumatic skin dermis, and lips to achieve the purpose of filling and repairing. field of bioengineering. Background technique

[0002] The methods usually applied to human soft tissue filling can be divided into dermal filling and subcutaneous filling. These fillings are not autologous tissue living materials in the past, but biosynthetic materials or heterosexua...

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