Injection liquid of human autogenous collagen living cell and preparation method thereof

A technology for collagen and injections, applied in peptide/protein components, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as insufficient volume, achieve no rejection, ideal long-term effects, and significant effects

Active Publication Date: 2008-10-01
黄靖西 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A kind of cell plastic surgery that appeared in recent years is a kind of better method, in " the application of skin tissue engineering technique in facial defects - autologous skin (children ) Stem Cell Cultivation, Injection and Storage" (by Huang Jingxi and Qin Junxia), in which the autologous skin fibroblasts are cultured and expanded in vitro, and then reinjected into the site that needs to be repaired, but because the injection only contains fibroblasts , the injection is relatively thin. It is okay to repair small wrinkles, but the volume is insufficient when filling large defects on the body surface, such as lip augmentation, labia augmentation, and penis enlargement.

Method used

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Examples

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Effect test

Embodiment Construction

[0010] The injection of the present invention is expanded and cultured in vitro by dermal fibroblasts derived from human body to produce collagen; the injection is obtained after synthesizing the collagen and the contained dermal fibroblasts.

[0011] The concrete preparation steps of this injection are:

[0012] 1. There are various extraction methods for the biological sample. It can be obtained from the umbilical cord that is separated from the mother when the fetus is born, or from the isolated skin discarded by the beauty lover when the eye bag is removed, or the scar tissue discarded during the scar excision. File and save the collected biological samples. The specific preservation method is: when the culture is established and reaches confluence, the fibroblasts can be sampled and frozen. It is better to freeze them sooner than later, because the passage times of normal fibroblasts are Limited; these fibroblasts can be stored in any culture medium suitable for preservin...

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PUM

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Abstract

This invention discloses a self-habitus collagen live cell injection and its preparing method comprising human body collagen and corium desmocyte. The preparation technique contains: outside expand and culture the corium desmocyte roots in human body to engender collagen; fetch the collagen and the corium desmocyte, now the injection is achieved. Compared with ancient injection for dispelling skin-dishing defect, the injection can fill up rather big defect by injecting it into the defect area, and its effect is very ideal; when the collagen injected is broken by collagenase, proteolytic enzyme and other enzymes, the desmocyte contained in the injection will produce new collagen. So the injection can keep the skin unfading and has long-tern ideal effect; the injection carries out the leap from variant to self-habitus.

Description

technical field [0001] The invention relates to an injection, in particular to a human autologous collagen living cell injection in biological tissue engineering. The invention also relates to a preparation method of the injection. Background technique [0002] With the passage of time, human skin will gradually become dull, lose elasticity, and finally form wrinkles. This is the most common skin depression defect, and skin depression defect also includes traumatic skin depression, non-traumatic skin depression such as Lip dysplasia, penis, labia dysplasia, skin stretch marks and other conditions. In the past, bovine collagen was mostly used to treat facial depression scars, but the immunogenicity of bovine collagen products limits the use of human body, and the mad cow disease that is prevalent abroad makes Chinese people daunted by imported products, which prompts some people to consider extracting human collagen from placenta . After clinical application, as Qin Junxia ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K38/39A61P17/16A61P43/00
Inventor 黄靖西秦军侠葛明明黄海
Owner 黄靖西
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