Device for separating tumor cells in blood fluid

Abstract

The invention provides a device for separating tumor cells in a blood fluid, which comprises a fluid inlet port, a first drive pump, a separating column and a fluid outlet port which are sequentially connected in series through a main circulating pipeline and can perform circulating flow, wherein the first drive pump is a peristaltic pump; the main circulating pipeline is provided with an anticoagulation normal saline injection port and an anticoagulation antagonist injection port; more than two subfiltration columns are arranged in the separating column; each subfiltration column is provided with a track etching film for separating tumor cells; the pore size of the track etching film is 5-25 mu m; the track etching film in each subfiltration column forms an independent stereoscopic filtration channel; the device also comprises a cell harvester; and the device successively constitutes a filtration operation channel and a separation operation channel. The device is a simplified invention of an invention patent in the prior art. The device can implement all the functions.

Description

technical field [0001] The invention relates to the field of cell separation in fluid, in particular to a device for separating tumor cells in blood fluid. Background technique [0002] In the prior art, when it is necessary to separate and detect cancer cells (circulating tumor cells, CTC) in the blood of living animals (including human beings), it is mostly by extracting a certain amount of blood, and then performing cell sorting by methods such as density gradient centrifugation. and count. Density gradient centrifugation is to use a certain medium to form a continuous or discontinuous density gradient in the centrifuge tube, place the cell suspension or homogenate on the top of the medium, and separate the cells by the action of gravity or centrifugal force field. But its shortcomings are obvious. Therefore, there is a need in the art for a new device and method for isolating and detecting the number of cancer cells in the blood of living animals. In recent years, a m...

AI Technical Summary

Problems solved by technology

However, the device corresponding to such a method can only separate cancer cells in a very small part of the blood of living animals at one time, but cannot effectively separate cancer cells in most or all of the blood of living animals, especially for larger living animals ( Such as the human body), this defect is particularly prominent

The reason is that in order to keep the animal in a normal living condition, it is impossible to radiate the whole body blood

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