Enrichment culturing method for anaerobic microorganisms

Abstract

The invention discloses an enrichment culturing method for anaerobic microorganisms. The method comprises the steps that after a culture medium is prepared, resazurin is added to serve as an oxidation-reduction indicator; the culture medium is put into a high-pressure sterilizing pot to be sterilized for 20-30 min at 121 DEG C and then taken out to be put into an ultra-clean bench to be cooled to 40 DEG C-60 DEG C for standby application; a deoxidant L-cysteine and sodium salt of the L-cysteine are added in the processed culture medium; a normal saline solution in a normal saline injection bottle is completely drawn with an injector needle, the normal saline injection bottle is cleaned with sterile water for 4-6 times and then placed on the ultra-clean bench, ultraviolet sterilizing is performed for 20-30 min, the culture medium and an inoculating sample are injected into the injection bottle, air in the bottle is completely drawn with an injector, the bottle is filled with high-purity nitrogen, the normal saline bottle is placed in a constant-temperature culturing box to be cultured for 2-5 days at 37 DEG C, and then enrichment culturing of the anaerobic microorganisms is completed. The enrichment culturing method for the anaerobic microorganisms does not need the help of an anaerobic culturing box and is convenient, fast and low in cost, and sterility in the whole course can be guaranteed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for enriching and culturing anaerobic microorganisms. Background technique [0002] In the process of cultivating anaerobic microorganisms, it is often necessary to use an anaerobic culture device to enrich and cultivate anaerobic microorganisms. However, the anaerobic incubator has the following disadvantages: (1) The operating cost is high, and nitrogen and mixed gas need to be continuously filled to maintain the normal operation of the anaerobic incubator; (2) In order to prevent the generation of ozone, it cannot be extinguished by ultraviolet light. (3) the alcohol lamp cannot be lit in the box during inoculation; (4) the operation is cumbersome and needs to be operated with long-arm gloves, which is very inconvenient. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a met...

AI Technical Summary

Problems solved by technology

However, the anaerobic incubator has the following disadvantages: (1) The operating cost is high, and nitrogen and mixed gas need to be continuously filled to maintain the normal operation of the anaerobic incubator; (2) In order to prevent the generation of ozone, it cannot be extinguished by ultraviolet light. (3) The alcohol lamp cannot be lit in the box during inoculation; (4) The operation is cumbersome and needs to be operated with long-arm gloves, which is very inconvenient