Method for preparing glyceride rich in fish oil and n-3 long-chain polyunsaturated fatty acid by virtue of enzyme method and product of method

A technology of unsaturated fatty acids and fatty acid glycerides, which is applied in the field of enzymatic preparation of n-3 long-chain polyunsaturated fatty acid glycerides rich in fish oil, which can solve the problem of limiting the added value of fish oil derivative products and the content of n-3 polyunsaturated fatty acids No high problems, to achieve the effect of digestion and absorption, high content

Pending Publication Date: 2020-05-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current research process generally uses fish oil directly as a raw material when preparing fish oil-derived diglycerides or (and) monoglycerides, and utilizes the method

Method used

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  • Method for preparing glyceride rich in fish oil and n-3 long-chain polyunsaturated fatty acid by virtue of enzyme method and product of method
  • Method for preparing glyceride rich in fish oil and n-3 long-chain polyunsaturated fatty acid by virtue of enzyme method and product of method
  • Method for preparing glyceride rich in fish oil and n-3 long-chain polyunsaturated fatty acid by virtue of enzyme method and product of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Tuna oil and ethanol are added in the batch reactor that can be sealed according to the ratio of substrate molar ratio of 1:3 (fish oil / ethanol), add the Lipozyme RM IM of 15wt%, be 25 ℃ at temperature, stirring speed is React for 4 hours under the condition of 400rpm, analyze the content of n-3 long-chain polyunsaturated fatty acids in the intermediate enzymatic hydrolysis product, and see Table 1 for the chemical composition of the intermediate enzymatic hydrolysis product.

[0026] Table 1

[0027]

[0028]

[0029] (2) After the ethanol and fatty acid ethyl ester of the intermediate enzymatic hydrolysis product are removed by vacuum distillation and molecular distillation, the intermediate enzymatic hydrolysis product and glycerin are added to the intermittent Add 3wt% Novozym 435 into the reactor, and react for 12 hours at a temperature of 70° C. and a stirring rate of 800 rpm to obtain a monoglyceride product. The composition of the obtained monoglyceri...

Embodiment 2

[0041] (1) Squid oil and ethanol are added in the sealable batch reactor according to the ratio of substrate molar ratio of 1:6 (fish oil / ethanol), adding 10wt% Lipozyme TL IM respectively, at a temperature of 30°C, stirring speed React under the condition of 600 rpm for 12 hours to obtain an intermediate enzymatic hydrolysis product rich in n-3 long-chain polyunsaturated fatty acids. The fatty acid composition of the obtained intermediate enzymatic hydrolysis product is shown in Table 6.

[0042] Table 6

[0043] fatty acid fish oil(%) Intermediate hydrolysis products (%) Glyceride composition content C14:0 6.4 2.4 Triglycerides 65.2 C16:0 14.7 7.3 diglycerides 30.3 C16:1 7.5 2.6 monoglyceride 4.5 C18:0 2.3 0.8 C18:1 17.3 8.4 C18:2 2.1 1.1 C18:3 2.4 1.2 C20:1 10.3 12.5 C20:4 1.9 4.1 C20:5(n-3)EPA 8.5 15.7 C22:1 4.1 8.5 C22:6(n-3)DHA 22.1 34.8 ...

Embodiment 3

[0056] (1) Add sardine oil and ethanol into a sealable batch reactor at a ratio of 1:9 (fish oil / ethanol) according to the substrate molar ratio, add 4wt% Lipase AY-30SD respectively, and stir at a temperature of 45°C The reaction was carried out for 8 hours under the condition of 800 rpm, and an intermediate enzymatic hydrolysis product rich in n-3 long-chain polyunsaturated fatty acids was obtained. The fatty acid composition of the obtained intermediate enzymatic hydrolysis product is shown in Table 11.

[0057] Table 11

[0058]

[0059]

[0060] (2) After the ethanol and fatty acid ethyl ester of the intermediate enzymatic hydrolysis product are removed by vacuum distillation and molecular distillation, the intermediate enzymatic hydrolysis product and glycerin are added to the intermittent Add 12wt% Novozym 435 into the reactor, and react for 6 hours at a temperature of 30° C. and a stirring rate of 400 rpm to obtain a monoglyceride product. The composition of th...

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Abstract

The invention discloses a method for preparing glyceride rich in fish oil and n-3 long-chain polyunsaturated fatty acid by virtue of an enzyme method and a product of the method. The method comprisesthe following steps: carrying out enzymatic alcoholysis reaction, so as to obtain an intermediate enzymolysis product rich in n-3 long-chain polyunsaturated fatty acid; adding the intermediate enzymolysis product and glycerin into an intermittent reactor in a molar ratio of 1 to (3-6), adding 3wt%-12wt% of lipase, and carrying out reaction at a temperature of 30-70 DEG C and a stirring velocity of300rpm-800rpm for 6-12 hours, so as to obtain a monoglyceride product; and adding the intermediate enzymolysis product and the glycerin into the intermittent reactor in a molar ratio of 1 to (0.5-2),adding 4wt%-14wt% of lipase, and carrying out reaction at a temperature of 30-70 DEG C and a stirring velocity of 300rpm-800rpm for 6-12 hours, so as to obtain a diglyceride product. The monoglyceride product or diglyceride product rich in n-3 long-chain polyunsaturated fatty acid is prepared through combination of enzymic-method alcoholysis enrichment and enzymic-method glycerolysis, the processoperation is simple, and the content of n-3 long-chain polyunsaturated fatty acid in the obtained diglyceride product or monoglyceride product is high.

Description

technical field [0001] The invention belongs to the technical field of oils and fats, in particular to a method for enzymatically preparing n-3 long-chain polyunsaturated fatty acid glycerides rich in fish oil and a product thereof. Background technique [0002] Deep-sea fish oil is the main source of n-3 long-chain polyunsaturated fatty acids (mainly eicosapentaenoic acid, EPA, C20:5n-3 and docosahexaenoic acid, DHA, C22:6n-3). n-3 long-chain polyunsaturated fatty acids have long been a healthy nutritional supplement with many health benefits, including improved cardiovascular health, brain function and overall health during pregnancy. EPA and DHA are non-essential n-3 fatty acids because the body can convert essential alpha-linolenic acid (ALA) into EPA and DHA. However, this conversion efficiency is not sufficient for the beneficial health effects of EPA and DHA. Therefore, obtaining these fatty acids from dietary sources is more necessary. Anchovies, salmon, herring, ...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6472C12P7/6454
Inventor 邹孝强张晖金青哲
Owner JIANGNAN UNIV
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