Method for preparing diglyceride by enzymatically catalyzing glycerol by supercritical CO2 system

A technology of lipase catalyzing glycerol and diglyceride, which is applied in the production and fermentation of bulk chemicals, can solve problems such as increased cost and reduced safety, and achieve the effects of increasing content, low viscosity and high solubility

Inactive Publication Date: 2011-01-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The use of chemical solvents improves the reaction rate to a certain extent, but it cannot fundamentally change the phenomenon of low enzymatic co

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0018] Specific implementation manner 1: The steps of this implementation manner are as follows:

[0019] Step 1: Add the first grade soybean oil and the glycerol dehydrated by molecular sieve into a 150mL stainless steel autoclave at a molar ratio of 1:1~3:1, and add a certain amount of lipase Lipozyme RM IM Stir quickly, add a small amount of water, seal the autoclave, and introduce CO 2 Perform a leak test, then use CO 2 Replace the air in the stainless steel autoclave. After the replacement is completed, control the temperature at 32℃ and charge CO 2 The pressure is 7.5MPa to ensure that the reaction process is in a supercritical state. After the heat-collecting thermostatic heating magnetic stirrer is heated to a certain temperature, the rotation speed is adjusted to a certain temperature, and the temperature is reacted for a period of time. After the reaction, the stainless steel autoclave was cooled to room temperature, the gas was released, the autoclave was opened, the ...

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PUM

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Abstract

The invention discloses a method for preparing diglyceride by enzymatically catalyzing glycerol by a supercritical CO2 system. Diglyceride (Diglyceride, DAG) is a natural ingredient of oil, has the content of about 5 percent, is an ingredient with stronger function in natrual oil and can be divided into two kinds of 1, 2-DAG and 1, 3-DAG according to different combination positions of acyl and glycerol hyroxyl, wherein the active function of the 1, 3-DAG is better. In recent years, by taking the natural oil as a raw material, the method for preparing the DAG comprises a glycerolysis method, a hydrolysis method, a hydrolysis-esterification combined method and the like. At present, the glycerolysis method is mainly adopted, but has low reaction yield and has little 1, 3-DAG content. In a supercritical state, a CO2 fluid simultaneously plays the roles of a solvent and a catalyst and can efficiently avoid the phenomena of catalyst positioning, inactivation and the like. In addition, moreimportantly, the CO2 fluid can prevent glycerolysis reverse reaction in the reaction environment. The diglyceride content in a product generated through the method is 70.2 percent, wherein the content of the 1, 3-DAG can reach 48.8 percent.

Description

Technical field: [0001] The invention relates to the field of edible oil processing, in particular to a method for preparing diglycerides by using carbon dioxide as a fluid and adopting lipase-catalyzed glycerolysis in a supercritical system. Background technique: [0002] With the improvement of people's living standards and the formation of bad living habits, obesity, cardiovascular disease, fatty liver and other wealthy diseases are becoming more and more common, which have seriously threatened human health. Diglycerides can not only improve the flavor of food and prolong the storage period, but also are the main additive of functional foods. It can reduce the accumulation of fat in the body and prevent and treat hyperlipidemia and related diseases. [0003] Diacylglycerol is abbreviated as diglyceride (DAG), which is obtained by esterification of glycerol and two fatty acids. It is a trace component in natural vegetable oils and an endogenous intermediate product of fat metabol...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCY02P20/54
Inventor 于殿宇王立琦江连洲胡立志李红玲侯俊才孙博陈晓慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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