Preparation method of diglyceride-enriched functional oil

A diacylglycerol and functional technology, which is applied in the field of preparation of functional oils rich in diacylglycerol, can solve the problems of unfavorable food system application, organic solvent residue, and many by-products, and achieve good industrial application prospects, The effect of product cost reduction and good catalytic effect

Inactive Publication Date: 2013-04-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reaction produces many by-products, and there may be residual organic solvents, which is not conducive to the application in food systems

Method used

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  • Preparation method of diglyceride-enriched functional oil
  • Preparation method of diglyceride-enriched functional oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 5 g of activated DA-201 macroporous adsorption resin, 10 g of Tris-HCl buffer solution, and 1 g of free phospholipase Lecitase ?Ultra mixed, stirred and adsorbed at 25°C for 2 hours, and then filtered to obtain a macroporous adsorption resin immobilized phospholipase with an enzyme activity of 1850 U / g. Vacuum dried and set aside.

[0031] Weigh 10g of refined soybean oil in a flask, place it in a constant temperature magnetic stirrer, add 100g of glycerol, 10g of water and 5g of immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 40° C., and react for 24 hours at a stirring speed of 200 r / min. After the reaction, the reaction mixture was poured into a centrifuge tube and centrifuged at 8000×g for 20 min. The upper oil layer is the functional oil rich in diglyceride, and the middle layer is the immobilized phospholipase Lecitase ? Ultra particles, the lower layer is unreacted glycerin. The upper oil layer was taken, and the diglycerid...

Embodiment 2

[0033] Weigh 5 g of activated DA-201 macroporous adsorption resin, 20 g of Tris-HCl buffer solution, and 1 g of free phospholipase Lecitase ? Ultra mixed, stirred and adsorbed at 30°C for 4 hours, and then filtered to obtain a macroporous resin-immobilized phospholipase with an enzyme activity of 1462 U / g. Vacuum dried and set aside.

[0034] Weigh 10g of refined palm oil in a flask, place in a constant temperature magnetic stirrer, add 80g of glycerin, 5g of water and 6g of immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 45° C., and react for 36 hours at a stirring speed of 300 r / min. After the reaction, the reaction mixture was poured into a centrifuge tube and centrifuged at 10000×g for 15 min. The upper oil layer is the functional oil rich in diglyceride, and the content of diglyceride is 51.7 wt% as determined by high performance liquid chromatography. After the phospholipase immobilized on the macroporous adsorption resin was reused 10 ti...

Embodiment 3

[0036] Weigh 5 g of activated DA-201 macroporous adsorption resin, 20 g of Tris-HCl buffer solution, and 1 g of free phospholipase Lecitase ? Ultra mixed, stirred and adsorbed at 35°C for 3 hours, and then filtered to obtain a macroporous adsorption resin immobilized phospholipase with an enzyme activity of 1344 U / g. Vacuum dried and set aside.

[0037] Weigh 10g of refined peanut oil in a flask, place it in a constant temperature magnetic stirrer, add 50g of glycerin, 8g of water and 8g of immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 35° C., and react for 24 hours at a stirring speed of 250 r / min. After the reaction, the reaction mixture was poured into a centrifuge tube and centrifuged at 6000×g for 30 min. The upper oil layer is the functional oil rich in diglyceride, and the content of diglyceride is 52.5 wt% as determined by high performance liquid chromatography. After the phospholipase immobilized on the macroporous adsorption resin w...

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Abstract

The invention relates to a preparation method of diglyceride-enriched functional oil. The method comprises following steps of: (1) immobilizing Lecitase Ultra; (2), carrying out a glycerolysis reaction on vegetable oil under the catalysis of immobilized Lecitase Ultra; and (3) carrying out product separation. The Lecitase Ultra used in the preparation method disclosed by the invention has low price which is only 5-10 percent of the price of lipase; and resin DA-201 for Lecitase Ultra immobilization has low cost. The immobilized Lecitase Ultra has good catalysis effect, high diglyceride content, less byproducts and recycling performance of the immobilized Lecitase Ultra; and therefore the diglyceride-enriched functional oil prepared by the preparation method disclosed by the invention has lower cost and favorable application prospect in the industry.

Description

technical field [0001] The invention relates to the enzymatic preparation of functional oil rich in diglyceride, in particular to a method for preparing functional oil rich in diglyceride by glycerolysis of vegetable oil. Background technique [0002] Diacylglycerol (DAG) is a structural lipid in which a fatty acid in a triacylglycerol (TAG) is replaced by a hydroxyl group. It is a trace component of natural vegetable oil and an endogenous intermediate product of fat metabolism in the body, and is a food ingredient generally recognized as safe (GRAS). In recent years, many medical studies have shown that diglycerides have the functions of regulating blood lipids, preventing arterial thrombosis, inhibiting liver fat accumulation, relieving diabetic nephropathy, reducing weight and suppressing appetite. In 2001, ADM Corporation, the largest grain and oil processing company in the United States, and Japan KAO (Kao) Corporation established a joint venture company ADM Kao LLC. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12N11/02
CPCY02P20/588Y02P20/50
Inventor 赵谋明崔春刘宁汪勇
Owner SOUTH CHINA UNIV OF TECH
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